3D Printing in Biomedical Research

Automation of Complex Patient-Derived Organoid (PDO) Culture and Screen

This paper demonstrates an automated platform for organoid culture and drug screening, showing improved efficiency and accuracy compared to manual methods.

SCAMPI: A scalable and accessible micropatterning platform for reproducible organoid morphogenesis

The paper introduces a scalable micropatterning platform for generating reproducible, geometrically controlled neural organoids.

Organoid Profiler: Automated, high-throughput and quantitative morphological characterization uncovers conserved longitudinal developmental kinetics in microfluidics-engineered organoids

The paper introduces Organoid Profiler, a deep-phenotyping pipeline that quantitatively characterizes microfluidics-engineered organoids, revealing conserved developmental kinetics and improved viability and homogeneity.

Network Modulation Enables 3D-Printed Citrate-Based Polymer Scaffolds with Broadly Tunable Mechanical Performance for Regenerative Engineering

This paper develops tunable citrate-based polymer scaffolds for regenerative engineering by modulating crosslinking density and network topology to achieve broad mechanical performance.

CombiCTx: Screening diffusion gradients of anti-cancer drug combinations

The paper introduces CombiCTx, an assay for screening anti-cancer drug combinations by simulating drug diffusion gradients in a tumor-like environment to assess interactions and cytotoxic effects.

Wax-printing-free fabrication of paper-supported 3D cancer cell culture

This paper introduces a wax-printing-free method for creating 3D cancer cell cultures on paper, enabling drug response analysis without hydrophobic patterning.

Predicting Human Pharmacokinetic Parameters of Drugs using a Multi-Tissue Chip Platform Integrating Liver, Kidney, and Skeletal Muscle Microphysiological Systems

This study develops a multi-tissue chip platform to predict human pharmacokinetic parameters of drugs without animal testing, showing accurate correlation with clinical data.

Integrating Microchannels and Flows into 3D Printable Granular Hydrogel Matrices

This paper develops a method to create stable microfluidic channels within granular hydrogels using selective crosslinking, enabling controlled perfusion and dynamic cellular environments.

Hydrogel array patterning using 3D-printed microfluidic inserts to control cell-cell and cell-ECM interactions

This paper introduces a 3D-printed microfluidic insert to pattern hydrogel arrays and generate gradients, enabling study of cell-cell and cell-ECM interactions in 3D microenvironments.

HYDRA: Fabrication of cell culture HYDrogels by Robotic liquid handling Automation for high-throughput drug testing

HYDRA is an automated method for creating hydrogel layers in HTS plates to improve drug testing by mimicking physiological conditions and avoiding meniscus formation.

An in vitro human vessel to study Neisseria meningitidis colonization and vascular damages

This paper develops a 3D microfluidic model to study Neisseria meningitidis colonization and vascular damage, showing it replicates in vivo infection features with higher resolution than animal or 2D models.

Advanced Bioprinting of Hydrogels with Controlled Mineral Gradients for Regenerative Engineering of the Osteochondral Interface

This paper develops a bioprinted hydrogel graft with a mineral gradient to mimic the osteochondral interface, showing structural and mechanical properties similar to native tissue.

Development and characterization of a biotechnological model suitable for studies of tumor cell extravasation and intravasation

The paper develops a biotechnological system to model tumor cell intravasation and extravasation, showing long-term cell viability and stable hydrodynamics under perfusion.

A workflow integrating organ-on-chip culture and correlative 3D light and electron microscopy for microtissue analysis

This paper presents a workflow combining organ-on-chip culture with 3D light and electron microscopy for microtissue analysis, demonstrating the feasibility of extended live imaging and focused ion beam/SEM for ultrastructural study.

Fabrication of Short Polymeric μFibers as Building Blocks for Anisotropic High-Throughput Compatible 3D Tissue Models

This paper develops scalable fabrication of anisotropic polymeric microfibers for 3D tissue models, showing they guide cell growth in high-throughput systems.

Self-assembly of hybrid 3D cultures by integrating living and synthetic cells

The paper develops hybrid 3D cancer cultures using synthetic and natural cells to mimic tumor immune microenvironments and identify immune evasion mechanisms.

Multi-scale Engineered Vasculature and Hierarchical Porosity via Volumetric Bioprinting-guided Photopolymerization-induced Phase Separation

This paper develops a method to create multi-scale vascular networks in hydrogels using volumetric bioprinting and phase separation, enabling capillary-like structures for nutrient delivery.

Systematic Growth Factor Profiling Platform for 3D Tumor Models Reveals Estradiol-Responsive Cellular Mechanisms of Immunotherapy Resistance

The paper develops a 3D tumor model platform with growth factor profiling to identify estradiol-responsive cellular mechanisms of immunotherapy resistance in ovarian cancer.

Collagen Hydrogel Tube Microbioreactors for Cell and Tissue Manufacturing

This paper introduces a collagen hydrogel tube microbioreactor system to improve cell culture scalability, robustness, and adhesion compared to alginate hydrogel tubes.

High-throughput Generation of Collagen Microbeads for 3D Cell Culture and Extracellular Vesicle Production

This study develops a microfluidic method to produce collagen microbeads for 3D cell culture and extracellular vesicle production, showing high throughput and cell compatibility.

Development of a Novel Hybprinter-SAM for Functionally Graded Tissue Engineering Constructs with Patterned and Localized Biochemical Signals

The paper develops a novel bioprinting system called Hybprinter-SAM to create functionally graded tissue constructs with patterned biochemical signals, demonstrating its application in bone-tendon regeneration.

A Sacrificial 3D Printed Vessel-on-Chip Demonstrates a Versatile Approach to Model Connective Tissue Pathology

This paper presents a 3D printed vessel-on-chip model that mimics connective tissue pathology by integrating perfused vasculature and immune cell interactions.

Pillar arrays as tunable interfacial barriers for microphysiological systems

This paper introduces a tunable interfacial barrier for microphysiological systems, demonstrating its use in cardiac tissue engineering and drug testing.

OrganoidChip+: a Microfluidic Platform for Culturing, Staining, Immobilization, and High-Content Imaging of Adult Stem Cell-Derived Organoids

The paper introduces OrganoidChip+, a microfluidic platform for culturing and high-content imaging of adult stem cell-derived organoids, showing improved growth rates and imaging capabilities compared to traditional methods.

Multidirectional alignment of collagen fibers to guide cell orientation in 3D-printed tissue

This paper develops a method to create 3D-printed collagen scaffolds with controlled fiber alignment patterns to guide cell orientation.

Biocompatibility assessment of SilkMA-Gelatin ink tuned for 3D extrusion bioprinting

This study develops and tests a SilkMA-Gelatin ink for 3D bioprinting, showing improved cytocompatibility, structural stability, and in vivo biocompatibility for tissue regeneration.

3D Hybrid Bioprinting for Complex Multi-Tissue Engineering

This paper introduces a 3D hybrid bioprinting method that combines soft and hard biomaterials to create mechanically robust and multifunctional constructs for tissue engineering.

3D bone printing via primed differentiation of stem cells with ultrasound (3DBonUS)

This paper introduces 3DBonUS, a 3D bone printing method using ultrasound and microfluidic bioprinting to direct stem cell differentiation, showing increased osteogenic markers and mineralization.

Remodeling of self-assembled microvascular networks under long term flow

This study shows that long-term flow can maintain and remodel microvascular networks in a microphysiological system for 51 days, reducing inflammation and enabling vascular remodeling.

Geometrical Designs in Volumetric Bioprinting to Study Cellular Behaviors in Engineered Constructs

This study examines how geometric designs in 3D bioprinted constructs affect cell behavior, showing geometry-sensitive responses in endothelial cells and geometry-insensitive growth in metastatic cancer cells.