Spectroscopy Techniques in Biomedical and Chemical Research

Optical metabolic imaging of the tricarboxylic acid cycle

This paper introduces MATRIX-SRS, a method to image TCA cycle activity in live cells, revealing metabolic changes during epithelial-to-mesenchymal transition.

RamanBot: Versatile high throughput Raman system

RamanBot is a low-cost automated Raman spectroscopy system that enables efficient multisample analysis with high stability and reliability.

Fix or Freeze? Spectral Differences Arising from Tissue Preparation in Chemical Imaging

This study compares spectral data from fresh frozen and formalin-fixed paraffin-embedded tissues using MIRSI, finding that preparation methods significantly alter chemical and morphological information.

Synthetic blood-based infrared molecular fingerprints: artificial cohorts for methodological research

The paper creates a synthetic infrared molecular fingerprint dataset for blood samples to enable methodological research without using real patient data.

Cell-in-Water: measurements using Optical Photothermal Infrared spectroscopy

This paper develops a method to perform O-PTIR microscopy on live cells in aqueous environments, showing that hydration preserves subcellular organization better than dehydration.

VIP-OT: Dissecting Single-Cell Biochemical State Dynamics under Perturbation via Vibrational Painting and Optimal Transport

VIP-OT traces drug response heterogeneity in single cells using vibrational imaging and optimal transport to predict post-treatment metabolic states from pre-treatment spectra.

RamanOmics Decodes Spatial Vibrational-Molecular Architecture and Rewiring in Aging and Repair

RamanOmics integrates snRNA-seq, spatial transcriptomics, and Raman imaging to map spatial biochemical and molecular changes in aging and repair, identifying senescent cell signatures and their functional roles.

Raman spectroscopy reveals growth phase-dependent molecular differences in bacterial membrane vesicles

This study uses Raman spectroscopy to analyze molecular changes in bacterial membrane vesicles across growth phases, revealing growth-dependent variations in protein-lipid ratios and secondary structure.

Automatic colocalization of high resolution MALDI MSI and Raman imaging applied to cardiac tissue of Fabry disease mouse models

The paper develops a multimodal imaging approach combining Raman and AP-MALDI MSI to detect Gb3 accumulation in cardiac tissue of Fabry disease mouse models.

Single-Cell Metabolic Imaging Reveals Glycogen Driven-Adaptations in Endothelial Cells

This study uses SRS microscopy to show that endothelial cells store glycogen under diabetes-like stress, which helps them survive glucose starvation and may offer new therapeutic targets for diabetes-related endothelial dysfunction.

RamanMAE: Masked Autoencoders Enable Efficient Molecular Imaging by Learning Biologically Meaningful Spectral Representations

RamanMAE uses masked autoencoders to learn spectral representations from Raman data, enabling efficient processing and noise reduction in biological imaging.

From Single Molecular Detail to Subcellular Dynamics: Real-Time Kinetics Study of dPA Uptake with Raman-based Spectroscopy

This study uses Raman and SRS spectroscopy to track dPA uptake in HL-60 cells, revealing subcellular lipid metabolism dynamics and heterogeneity.

Label-Free Longitudinal Imaging of Single Cell Drug Response with a 3D-Printed Cell Culture Platform

This paper presents a 3D-printed cell culture platform for label-free longitudinal imaging of single-cell drug responses using SRS microscopy, enabling real-time monitoring and analysis of biochemical and morphological changes.

Fresh Paraformaldehyde Preserves Thrombus Biochemistry -Infrared Spectroscopy and Secondary Ion Mass Spectrometry Investigation of Pulmonary Artery Thrombi

This study quantifies how aged paraformaldehyde affects thrombus molecular integrity using spectroscopy and shows a QC workflow to ensure consistency in fixative quality.

Texture-Analysis Classification of Intraductal Carcinoma of the Prostate Using Label-Free Multimodal Nonlinear Optical Imaging

This study uses nonlinear optical imaging and SVM classification to accurately distinguish intraductal carcinoma of the prostate from other tissue types with high accuracy.

Mid-Infrared Photothermal Mesoscopy with Millimeter Field of View and Sub-micron Spatial Resolution

This paper introduces a mid-infrared photothermal mesoscope with millimeter-scale field of view and sub-micron resolution, enabling in vivo chemical imaging and Alzheimer's disease biomarker detection.

Open Raman Microscopy (ORM): A Modular Hardware and Software Framework for Accessible Raman Imaging

The paper introduces an open-source Raman microscopy framework with modular hardware and software for accessible chemical imaging across various applications.

Normalized Raman Imaging for Studies of Tissue Physiology of the Kidney

This paper introduces NoRI, a Raman imaging technique that enables quantitative, label-free analysis of kidney tissue, revealing sex-specific differences and dynamic changes in acute kidney injury.

Imaging Cellular Metabolic Rewiring with SuMMIT-SRS

The paper introduces SuMMIT-SRS, a multiplexed metabolic imaging technique, and demonstrates its application in various cell types to study metabolic rewiring.

Ultrastructural Analysis of Human Uncinate Fasciculus with Spectral-Focusing Coherent Anti-Stokes Raman Spectroscopy

The paper develops and validates a novel sf-CARS system with AxonDeepSeg to analyze UF ultrastructure in human postmortem brain tissue, finding mean axon diameter and myelin thickness.

4-Pi Stimulated Raman Scattering for Label-free Super-resolution Chemical Imaging

This paper introduces 4Pi-SRS, a label-free super-resolution imaging technique that improves axial resolution by combining SRS with 4Pi-interferometry, enabling high-resolution imaging of lipid structures in cells.

Combining quantum cascade lasers and plasmonic metasurfaces to monitor de novo lipogenesis with vibrational contrast microscopy

This paper introduces MIRIAM, a label-free microscopy technique using quantum cascade lasers and plasmonic metasurfaces to monitor de novo lipogenesis in cells with vibrational contrast.

Covalent Drug binding in Live Cells Monitored by Mid-IR Quantum Cascade Laser Spectroscopy: Photoactive Yellow Protein as a Model System

This paper develops a mid-IR quantum cascade laser spectroscopy method to detect drug-target interactions in live cells using photoactive yellow protein as a model system.

Morphomolecular Longitudinal Endoscopy of Carcinogenesis

This paper introduces a label-free endoscopic platform combining Raman spectroscopy and OCT to track morphomolecular changes in real-time during carcinogenesis, enabling early cancer detection.

Real-time monitoring of attenuated cytomegalovirus using Raman spectroscopy allows non-destructive characterization during flow

This paper develops a Raman spectroscopy tool for real-time monitoring of attenuated CMV in flow, demonstrating its ability to detect viral particles at high flow rates and across concentrations.

Detection of Unlabeled Micro- and Nanoplastics in Unstained Tissue with Optical Photothermal Infrared Spectroscopy

This study demonstrates the use of O-PTIR spectroscopy for label-free detection of micro- and nanoparticles in tissues, showing superior resolution and signal quality compared to FTIR.

CIAdex: Single-Cell FTIR Spectral Fingerprinting for Cell Identity Verification and Aging Quantification in Therapeutic Cell Manufacturing

CIAdex uses single-cell FTIR spectral fingerprints and machine learning to verify cell identity and quantify aging in therapeutic cell manufacturing.

Marker-independent imaging reveals a correlation of fibrotic and epigenetic alterations in endometriosis

This study uses Raman microspectroscopy to identify fibrotic and epigenetic changes in endometriosis, showing potential for non-invasive biomarker discovery and diagnostic applications.

Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy

This paper introduces an oblique photothermal microscope (OPTM) for ultrasensitive in vivo IR spectroscopic imaging with sub-micron resolution, enabling low-dose imaging of skin and tracking of topical drugs.

Label-Free Quantification of Apoptosis and Necrosis Using Stimulated Raman Scattering Microscopy

This study uses stimulated Raman scattering microscopy to rapidly and label-free monitor apoptotic and necrotic transitions in CHO cells, identifying distinct subpopulations and responses to stressors.