D. Goldfarb

A basally active cGAS-STING pathway limits SARS-CoV-2 replication in a subset of ACE2 positive airway cell models

This study identifies tonic activation of the cGAS-STING and IFN pathways in ACE2-positive airway cells as a factor limiting SARS-CoV-2 replication.

Poly-basic peptides and polymers as new drug candidate against Plasmodium falciparum

This study shows poly-lysine peptides and their modifications can reduce Plasmodium falciparum in human erythrocytes in vitro, with potential as new antimalarial drugs.

Biophysical Mechanism of Allosteric Regulation of Actin Capping Protein

This study investigates the biophysical mechanism of allosteric regulation of actin capping protein using single-molecule FRET and MD simulations, revealing conformational changes linked to ligand binding.

Targeted proteomic quantitation of NRF2 signaling and predictive biomarkers in HNSCC

This paper develops a targeted proteomics assay to quantify NRF2 pathway components and clinical biomarkers in HNSCC, identifying correlations between NRF2 activity, HPV infection, and immune markers.

Peptide-RNA Coacervates as a Cradle for the Evolution of Folded Domains

This study investigates how peptide-RNA coacervates influence the structure and dynamics of the HhH motif, suggesting they may have facilitated early protein evolution through oligomerization.

Nanotrap Particles Improve Nanopore Sequencing of SARS-CoV-2 and Other Respiratory Viruses

The paper introduces a magnetic hydrogel particle method to improve nanopore sequencing of SARS-CoV-2 and other respiratory viruses from diagnostic samples.

Protein proximity networks and functional evaluation of the Casein Kinase 1 γ family reveals unique roles for CK1γ3 in WNT signaling

This study identifies unique roles for CK1γ3 in WNT signaling through proximity biotinylation, functional assays, and inhibitor testing, showing its distinct activation of β-catenin and Notch pathways.

In vitro coinfection by influenza A virus and respiratory syncytial virus generates hybrid viral particles with altered structure and tropism

This study shows that co-infection of human lung cells with influenza A and respiratory syncytial viruses produces hybrid viral particles with altered structure and immune evasion capabilities.

Systematic analysis of SARS-CoV-2 infection of an ACE2-negative human airway cell

This study identifies H522 cells as ACE2-negative human cells permissive to SARS-CoV-2 infection, revealing alternative receptor usage and host response mechanisms.

Elevated temperature inhibits SARS-CoV-2 replication in respiratory epithelium independently of the induction of IFN-mediated innate immune defences

This study shows that elevated temperatures inhibit SARS-CoV-2 replication in respiratory epithelium without triggering IFN-mediated immune responses.

The ubiquitylome of developing cortical neurons

This study identifies ubiquitylated substrates of TRIM9 and TRIM67 in developing cortical neurons using ubiquitin remnant profiling.

The TRIM9/TRIM67 neuronal interactome reveals novel activators of morphogenesis

This study identifies novel interactors of TRIM9 and TRIM67 using BioID and validates their roles in neuronal morphogenesis and axonal dynamics.

Drug binding sites in the multidrug transporter MdfA in detergent solution and in lipid nanodiscs

This paper uses DEER spectroscopy to determine the binding site of a drug analog in MdfA and examines conformational changes in detergent micelles and lipid nanodiscs.

In-cell destabilization of a homo-dimeric protein complex detected by DEER spectroscopy

This study uses DEER spectroscopy to show that a homo-dimeric protein complex is destabilized in human cells compared to solution, challenging excluded volume crowding theory.

Nanotrap(R) particles improve detection of SARS-CoV-2 for pooled sample methods, extraction-free saliva methods, and extraction-free medium methods

This paper introduces a method using Nanotrap(R) particles to improve SARS-CoV-2 detection in pooled samples, saliva, and transport medium by concentrating virus before RNA extraction.

Altered conformational sampling along an evolutionary trajectory changes the catalytic activity of an enzyme

The paper investigates how remote mutations alter conformational sampling along an evolutionary trajectory, increasing catalytic activity in an enzyme.

TRIM67 Regulates Exocytic Mode and Neuronal Morphogenesis via SNAP47

The paper identifies four exocytic fusion modes in developing neurons and shows TRIM67 promotes FVF-like exocytosis by limiting SNAP47 incorporation into SNARE complexes.

Wnt Regulation: Exploring Axin-Disheveled interactions and defining mechanisms by which the SCF E3 ubiquitin ligase is recruited to the destruction complex

The paper investigates Wnt signaling by examining Axin-Dishevelled interactions and the recruitment of the SCF E3 ubiquitin ligase to the destruction complex, revealing dynamic roles of Axin and Dishevelled.

Gain-of-function genetic screen of the kinome reveals BRSK2 as an inhibitor of the NRF2 transcription factor

The paper identifies BRSK2 as a negative regulator of NRF2 by inhibiting protein synthesis through mTOR downregulation, suggesting it as a potential therapeutic target for NRF2-related diseases.