M. Mann

Furin cleavage of the SARS-CoV-2 spike is modulated by O-glycosylation

This study shows that O-glycosylation near the furin cleavage site of SARS-CoV-2 spike protein reduces furin cleavage and is mediated by GALNT enzymes and a proline at position 681.

Spatially and cell-type resolved quantitative proteomic atlas of healthy human skin

The paper creates a spatially and cell-type resolved proteomic atlas of healthy human skin, quantifying 10,701 proteins across different locations and cell types.

A paired liver biopsy and plasma proteomics study reveals circulating biomarkers for alcohol-related liver disease

This study identifies circulating proteomic biomarkers for alcohol-related liver disease using paired liver biopsy and plasma proteomics, showing improved performance over existing tests.

Defining the RBPome of T helper cells to study higher order post-transcriptional gene regulation

The paper identifies the core RNA-binding protein (RBP)ome in human and mouse T helper cells, revealing new regulators of post-transcriptional gene regulation, including Celf1 as a Roquin-1-dependent coregulator of Icos.

Purification of age-distinct insulin secretory granules through antigen restriction

The paper purifies age-distinct insulin secretory granules from INS-1 cells and identifies age-related changes in their lipid composition and membrane properties.

Gain-of-function mutations amplify cytotoxic FAM111 protease activity in human genetic disorders

The paper shows that gain-of-function mutations in FAM111A and FAM111B enhance protease activity, leading to DNA replication and transcription suppression and cell death, contributing to genetic disorders.

Limited Environmental Serine Confers Sensitivity to PHGDH Inhibition in Brain Metastasis

This study shows that brain metastases rely on PHGDH-mediated serine synthesis due to limited environmental serine, making them sensitive to PHGDH inhibition.

Amyloid-like aggregates cause lysosomal defects in neurons via gain-of-function toxicity

This study shows that amyloid-like aggregates impair lysosomal function in neurons by sequestering AP-31, leading to neurotoxicity.

Effects of N-Acetyl-Leucine and its enantiomers in Niemann-Pick disease type C cells

This study evaluates the effects of N-Acetyl-Leucine and its enantiomers on lysosomal volume in NPC cells, finding that N-Acetyl-L-Leucine is most effective.

Accurate MS-based Rab10 phosphorylation stoichiometry determination as readout for LRRK2 activity in Parkinson’s disease

The paper develops an ultra-sensitive MS-based assay to measure Rab10-Thr73 phosphorylation stoichiometry as a biomarker for LRRK2 activity in Parkinson’s disease.

STAT3-dependent systems-level analysis reveals PDK4 as an independent predictor of biochemical recurrence in prostate cancer

The paper identifies PDK4 as an independent prognostic marker for prostate cancer recurrence using gene co-expression networks and proteomics data.

Proteomics-based comparative mapping of the human brown and white adipocyte secretome reveals EPDR1 as a novel batokine

The paper compares the secretomes of human brown and white adipocytes using proteomics, identifying EPDR1 as a novel batokine involved in thermogenesis and energy consumption.

The CMG helicase bypasses DNA protein cross-links to facilitate their repair

The paper shows that the CMG helicase bypasses DNA-protein cross-links without prior proteolysis, and RTEL1 aids this process and also promotes proteolysis.

A novel LC system embeds analytes in pre-formed gradients for rapid, ultra-robust proteomics

The paper introduces a novel LC system for proteomics that enables rapid, high-throughput, and robust analysis with deep proteome coverage.

EASI-tag enables accurate multiplexed and interference-free MS2-based proteome quantification

EASI-tag enables accurate multiplexed proteome quantification by generating interference-free reporter ions through dissociation at low collision energy.

Heterogeneous Responses of Hematopoietic Stem Cells to Inflammatory Stimuli are Altered with Age

The paper shows that aged hematopoietic stem cells exhibit a myeloid-biased transcriptional response to inflammation, which is linked to Klf5, Ikzf1, and Stat3.