Stefano Forli

Autophagy activator AA-20 engages CDK5 to promote lipophagy and improve neurodegeneration-associated phenotypes

AA-20 promotes lipophagy by activating lysosomal acid lipase and CDK5, reducing lipid droplet accumulation and improving neurodegeneration-associated phenotypes in cellular and organoid models.

Genetic and pharmacological inactivation of peptidoglycan remodeling increases antibiotic susceptibility of vancomycin-resistant Enterococcus faecium

This study shows that inhibiting SagA, a peptidoglycan hydrolase, increases vancomycin susceptibility in VREfm through both genetic and pharmacological approaches.

The SLC15A4-LAMTOR1 interaction licenses endolysosomal TLR-mediated mTOR signaling and inflammatory cytokine production

This paper identifies a direct interaction between SLC15A4 and LAMTOR1 that is required for endolysosomal TLR-mediated mTOR signaling and inflammatory cytokine production.

Post-Translational Modifications Remodel Proteome-Wide Ligandability

The paper identifies how post-translational modifications affect protein ligandability by profiling PTM-dependent small-molecule interactions in human cells.

Pharmacological Inhibition of SLC33A1 Promotes Endoplasmic Reticulum Hyperoxidation and Induces Adaptive IRE1/XBP1s Signaling

This study shows that IXA4 inhibits SLC33A1, causing ER hyperoxidation and activating IRE1/XBP1s signaling, which reduces viability of KEAP1-deficient lung cancer cells.

Design principles of the common Gly-X6-Gly membrane protein building block

The paper uses protein design to investigate the structural principles of common antiparallel transmembrane motifs, revealing how sequence patterns stabilize membrane protein architectures.

Structural insights into IMP2 dimerization and RNA binding

This study determines the structural properties of full-length IMP2, revealing its dimeric conformation and RNA-induced changes, which may inform drug design.

De novo designed pMHC binders facilitate T cell induced killing of cancer cells

This paper designs de novo pMHC binders using generative models to create T cell-based cancer therapeutics with high specificity and minimal cross-reactivity.

Microbiota-derived secondary bile acids promote STING activation and antitumor activity

This study identifies DCA as a microbiota-derived bile acid that activates STING and enhances antitumor immunity via NK cell activation.

An mRNA Display Approach for Covalent Targeting of a Staphylococcus aureus Virulence Factor

The paper develops fluorescent probes for FphB, a Staphylococcus aureus enzyme, using mRNA display with a genetically encoded electrophile, achieving nanomolar selectivity.

The conformational landscape of human transthyretin revealed by cryo-EM

This study uses cryo-EM to reveal the conformational landscape of human transthyretin, uncovering asymmetries and new states relevant to ligand binding and amyloid formation.

IP6 and PF74 affect HIV-1 Capsid Stability through Modulation of Hexamer-Hexamer Tilt Angle Preference

This study uses atomistic simulations to show how IP6 and PF74 modulate HIV-1 capsid stability by altering hexamer-hexamer tilt angles, revealing distinct effects on structural flexibility.

Assessing AF2's ability to predict structural ensembles of proteins

This study evaluates AF2's ability to predict protein structural ensembles by comparing its results with MD simulations and NMR data for four test proteins.