imputation

High Numerical Aperture Epi-illumination Selective Plane Illumination Microscopy

This paper introduces a high numerical aperture epi-illumination SPIM system for fast and high-resolution volumetric imaging of live cells and single-molecule super-resolution microscopy.

Multidirectional digital scanned light-sheet microscopy enables uniform fluorescence excitation and contrast-enhanced imaging

Glaser et al. describe a light-sheet microscopy architecture that enables passive multidirectional illumination with confocal line detection to enable both uniform fluorescence excitation and contrast-enhanced imaging of fluorescently labeled samples.

Characterizing and Managing Missing Structured Data in Electronic Health Records

The paper evaluates imputation methods for missing structured data in EHRs using clinical lab measures from 602,366 patients, showing some methods perform well while others are unsuitable for multiple imputation.

HiCPlus: Resolution Enhancement of Hi-C interaction heatmap

HiCPlus enhances the resolution of Hi-C interaction heatmaps using deep learning, reconstructing high-resolution data from low-resolution inputs.

Photon yield enhancement of red fluorophores at cryogenic temperatures

This study examines how cooling fluorescent dyes to cryogenic temperatures increases photon yield and improves localization precision in single molecule microscopy.

GSimp: A Gibbs sampler based left-censored missing value imputation approach for metabolomics studies

GSimp is a Gibbs sampler-based method for imputing left-censored missing values in metabolomics data, showing superior performance in accuracy and statistical analysis.

Enhanced photon collection enables four dimensional fluorescence nanoscopy of living systems

The paper introduces MoNaLISA, a nanoscope that enables 4D fluorescence nanoscopy of living systems with enhanced photon collection, achieving sub-100 nm resolution at low light intensities.

X10 Expansion Microscopy Enables 25 nm Resolution on Conventional Microscopes

This paper introduces X10 expansion microscopy, achieving 25-30 nm resolution on conventional microscopes by improving the gel recipe for a 10x expansion factor.

Missing Value Imputation Approach for Mass Spectrometry-based Metabolomics Data

The paper compares imputation methods for metabolomics data with missing values and proposes a strategy with a web-tool.

netSmooth: Network-smoothing based imputation for single cell RNA-seq

netSmooth improves scRNA-seq clustering by smoothing expression values using network diffusion and gene expression priors.

MAGIC: A diffusion-based imputation method reveals gene-gene interactions in single-cell RNA-sequencing data

MAGIC improves single-cell RNA-seq data by imputing missing values and revealing gene-gene interactions through a diffusion-based method.

Sequential Super-Resolution Imaging using DNA Strand Displacement

This paper introduces a method for sequential super-resolution imaging using DNA strand displacement to label and remove fluorophores, enabling multiplex imaging of subcellular structures.

Siccuracy: An R-package for executing genotype imputation strategy simulations with AlphaImpute

Siccuracy evaluates genotype imputation accuracy using correlation and counts, demonstrating that range of correlations better reflects performance than average correlation.

In vivo multiphoton fluorescence imaging with polymer dots

This paper demonstrates the use of polymer dots for deep in vivo multiphoton imaging in mice, showing improved signal-to-background ratio and imaging depth.

Optical crosstalk in SPAD arrays for high-throughput single-molecule fluorescence spectroscopy

The paper examines optical crosstalk in SPAD arrays used for high-throughput single-molecule fluorescence spectroscopy and finds it negligible and undetectable in data analysis.

A genetically encoded Ca2+ indicator based on circularly permutated sea anemone red fluorescent protein

The paper develops and validates a new red fluorescent calcium indicator, K-GECO1, based on a circularly permutated RFP from a sea anemone, tested across multiple cell types and organisms.

A toolbox of anti-mouse and rabbit IgG secondary nanobodies

This paper introduces sustainable nanobodies against mouse and rabbit IgG as alternatives to traditional animal-derived secondary antibodies, demonstrating their utility in various imaging and detection applications.

A method for allocating low-coverage sequencing resources by targeting haplotypes rather than individuals

This paper introduces AlphaSeqOpt, a method that allocates low-coverage sequencing resources by targeting haplotypes to improve phasing and imputation accuracy.

A spontaneously blinking fluorescent protein for simple single laser super-resolution live cell imaging

The paper introduces a fluorescent protein, SPOON, that spontaneously blinks in response to light and dehydration, enabling simple single-laser super-resolution imaging.

LITE microscopy: a technique for high numerical aperture, low photobleaching fluorescence imaging

The paper introduces LITE microscopy, a technique that improves resolution and brightness while reducing photodamage in fluorescence imaging.

Single-shot super-resolution total internal reflection fluorescence microscopy

This paper introduces a method combining instant structured illumination microscopy with TIRF to achieve higher resolution and faster imaging of live samples near the coverslip.

Dynamic, non-contact 3D sample rotation for microscopy

This paper introduces a method for dynamically rotating 3D samples using magnetic fields for improved in vivo microscopy imaging in zebrafish.

3D Single-Molecule Super-Resolution Microscopy With A Tilted Light Sheet

This paper introduces TILT3D, a 3D single-molecule super-resolution microscopy technique that enables high-precision imaging in thick cells using tilted light sheets and specialized PSFs.

Genome-wide genetic data on ~500,000 UK Biobank participants

Quality-controlled genotypes for 488,377 UK Biobank participants at 805,426 markers, imputed to ~96 million variants, plus ancestry PCs, kinship (30.3% have close relatives enrolled) and HLA calls.

Multiplexed Live-Cell Visualization Of Endogenous Proteins With Nanometer Precision By Fluorobodies

This paper introduces fluorobodies, engineered nanobodies with bright fluorophores, enabling high-precision live-cell imaging of endogenous proteins with low background and toxicity.

Boosting The Localization Precision Of dSTORM By Biocompatible Metal-Dielectric Coated Glass Coverslips

This paper improves dSTORM localization precision by using biocompatible metal-dielectric coated glass coverslips, enhancing signal-to-noise ratio by a factor of two.

High-Density Super-Resolution Microscopy With An Incoherent Light Source And A Conventional Epifluorescence Microscope Setup

This paper demonstrates high-density single-molecule super-resolution microscopy using a conventional epifluorescence microscope and incoherent light, achieving 90 nm resolution on mouse and amphibian meiotic chromosomes.

Shortwave Infrared Fluorescence Imaging with the Clinically Approved Near-Infrared Dye Indocyanine Green

This paper demonstrates that FDA-approved NIR dyes like ICG can be used for SWIR fluorescence imaging in vivo, enabling high-contrast imaging beyond 1500 nm with potential clinical applications.

A Versatile Compressed Sensing Scheme For Faster And Less Phototoxic 3D Fluorescence Microscopy

This paper introduces a 3D compressed sensing method for fluorescence microscopy that reduces light exposure and acquisition time by 5-10 fold without hardware changes.

A fluorogenic nanobody array tag for prolonged single molecule imaging in live cells

This paper introduces a fluorogenic nanobody array tag, ArrayG, enabling prolonged single molecule imaging in live cells with reduced background and improved photo-stability.