DNA Repair Mechanisms

This study shows that reducing R-loops in Brca1-knockout mice increases ER-expressing tumors but does not affect tumor incidence.

This study identifies the role of ATM and 53BP1 in regulating alternative end joining-mediated V(D)J recombination by analyzing repair outcomes of RAG1/2-initiated DSBs.

This study identifies RBM10's role in DNA replication and shows that targeting WEE1 can sensitize RBM10-deficient lung adenocarcinoma cells.

This study investigates DNA damage tolerance mechanisms in Drosophila melanogaster, showing that translesion synthesis is preferred over template switching for alkylation damage, with REV1 playing a critical role in coordinating this response.

The paper identifies mechanisms that limit Ku protein accumulation in chromatin, showing DNA-PKcs blocks Ku entry, and two additional mechanisms restrict Ku levels in the absence of DNA-PKcs.

The paper shows that germ cells in Drosophila tolerate DNA damage during meiosis but are sensitive to it during mitosis, revealing a threshold for DNA damage tolerance in germline development.

This study identifies STK19 as a critical factor in transcription-coupled nucleotide excision repair by stabilizing the repair complex and promoting Pol II ubiquitylation and TFIIH recruitment.

This study shows that CMG helicase disassembly is essential in budding yeast and occurs via two pathways involving ubiquitylation and Rrm3/Pif1 helicases.

This study uses an aiHumanoids platform to simulate XPA disease progression, showing increased DNA damage, genomic instability, cancer risk, and neurological issues in XPA individuals under UV exposure.

The paper uses XR-seq to map transcription events in C. elegans by analyzing repair in XPC mutants, revealing previously unrecognized transcription sites.

This study identifies a novel regulatory mechanism for WRN degradation via the PIAS4-RNF4-p97/VCP axis in MSI-H cancer cells using single molecule tracking and inhibitor screening.

This study identifies PolX enzymes as essential for processing DNA ends during programmed genome rearrangement in Paramecium tetraurelia, revealing their role in NHEJ and IES excision.

MADDD-seq detects DNA damage and mutations simultaneously in yeast using a modified double barcoding sequencing approach, revealing repair kinetics and mutagenesis over 24 hours.

The paper develops a mathematical model to describe transcription loss due to accumulated DNA damage and analyzes its asymptotic behavior and first hitting time using mice data.

This study shows eEF2K is critical for the DNA damage response to cisplatin, promoting p53 activation and DNA repair depending on damage severity.

This study shows that lesion-stalled RNA polymerase II can be evicted by the p97-proteasome pathway independently of repair, and both processes require CSA and ubiquitination.

The paper develops a method to map Pol κ activity across the genome in human fibroblasts and finds it is enriched in euchromatin, gene promoters, and early S-phase replicated DNA.

This study identifies a novel phosphorylation motif used by DNA-PKcs, revealing its role in regulating splicing factors and transcriptional regulators across species.

This study investigates the DNA binding and bridging activities of CtIP in healthy and diseased states, identifying a C-terminal DNA-binding domain and showing that tetramerization is required for DNA bridging.

The paper studies DNA double-strand breaks (DSBs) during nematode programmed DNA elimination, showing that DSBs are processed with end resection and telomere addition, with biased repair on retained ends.

A multiplexed screen reveals how dozens of proteins sense the local chromatin context to tune the balance between two DNA repair pathways.

The paper investigates how Srs2 helicase and Rrp1/Rrp2 translocases affect replication stress response in fission yeast, showing differential checkpoint activation with overproduction.

The paper shows Y RNA-derived fragments packaged with YBX1 enhance PARP1 residency at DNA double strand breaks, improving DNA repair and cell survival through ADP-ribosylation.

This study shows that HSR-9, the C. elegans ortholog of 53BP1, regulates meiotic recombination and X chromosome segregation, with both conserved and unique functions compared to somatic cells.

The paper identifies Msc1 as a nuclear envelope protein that enhances DNA repair in late mitosis by supporting homologous recombination.

The paper investigates how strand-asymmetric processes like replication and transcription influence DNA damage, repair, and mutagenesis, revealing mechanisms that shape cancer genome evolution.

The paper uses single-molecule imaging to reveal how two CMG helicases are assembled and activated at replication origins in metazoan genomes.

The paper investigates how SUMO protease and proteasome recruitment at nuclear pores affects replication dynamics at arrested forks in fission yeast.

The paper examines RAD51 transcriptional response and cell cycle dynamics in Arabidopsis root cell types under DNA damage, revealing differences in sensitivity to genotoxic stress.

The paper shows that Mus81 and Yen1 cleave D-loops to form half-crossover precursors in budding yeast.