Pluripotent Stem Cells Research

This study investigates how somatic mutations affect dopaminergic neuron differentiation in iPSCs, finding that deleterious mutations in key genes like BCOR are linked to differentiation failure and altered proliferation.

This study shows that Wnt/TCF7L1 repression promotes primitive endoderm differentiation in mESCs by inhibiting naive and formative pluripotency factors.

This study identifies a redox-sensitive cysteine residue in Oct4 that regulates reprogramming and differentiation by affecting DNA binding and ubiquitylation.

The paper identifies small molecules that enhance stem cell differentiation into definitive endoderm, reducing costs by 90% through a drug repurposing approach.

This study develops a workflow to generate and analyze iPSC-derived motor neuron spheroids, confirming their marker expression and functional activity.

This study uses iPSC-derived dopaminergic neurons to model THD, showing disease-specific phenotypes and treatment responses.

This study establishes methods to generate marmoset primordial germ cell-like cells from iPSCs, revealing a primate-specific germline transcriptional network and potential for in vitro gametogenesis modeling.

This study identifies RSL24D1 as a key regulator of ribosome biogenesis and pluripotency in mouse embryonic stem cells, showing its role in maintaining self-renewal and suppressing differentiation.

This study generates functional ovarian granulosa-like cells from human iPSCs by overexpressing NR5A1 and RUNX1 or RUNX2, enabling the formation of ovary-like organoids with germ cells.

The paper shows NANOGP1, a duplicate of NANOG, has partial functional conservation in human naive pluripotent stem cells, contributing to pluripotency but not maintaining undifferentiated status.

This paper describes a 14-day protocol to differentiate human iPSCs into cortical neural stem cells, validated through RNA-Seq and immunocytochemistry.

This study combines Ngn2 overexpression with small molecule patterning to efficiently generate lower induced Motor Neurons (liMoNes) from hPSCs, achieving high yields and functional characteristics.

This study generates T-cell progenitors from human pluripotent stem cells to produce functional thymocytes, demonstrating similarity to cord blood HSCs and DN3a thymocytes.

The paper shows STAT3 and TFCP2L1 are essential for epiblast self-renewal during diapause, but only TFCP2L1 is needed for deriving embryonic stem cells in vitro.

The paper identifies ETV2 dynamics in hiPSC-derived cardiac lineages using single-cell RNA-seq and a fluorescent reporter, revealing distinct subpopulations for endothelial and cardiomyocyte specification.

The paper identifies lipid desaturation as a metabolic regulator of self-renewal and differentiation in mouse blastocyst-derived stem cells, showing distinct effects on embryonic stem cells (ESCs) and trophoblast stem cells (TSCs).

The paper identifies retrotransposon insertions as the main source of mutations in mouse induced pluripotent stem cells, showing hypomethylation of LINE-1 elements and their impact on gene regulation.

The paper identifies and characterizes a reference iPSC line, KOLF2.1J, for standardized use in collaborative studies across multiple differentiation protocols and assays.

This study shows that activating RARβ and RARγ receptors with specific ligands enhances cell specialization during stem cell differentiation into neuronal and glial cell types.

This study identifies YAP suppression as the mechanism by which tankyrase inhibition maintains human naive pluripotency without inducing differentiation.

This study generates transplantable human thyroid organoids from embryonic stem cells that restore thyroid hormone levels in athyreotic mice.

This study improves human embryonic stem cell differentiation into midbrain dopaminergic neurons using specific factors and reveals developmental dynamics and high-quality cell types through single-cell RNA-sequencing.

This study generates human fetal zone adrenal cortex-like cells from induced pluripotent stem cells, revealing key signaling pathways and transcription factors involved in adrenocortical specification and steroidogenesis.

This study compares three cortical organoid protocols to assess brain regional identity, cell type specificity, and neuronal maturation, finding that directed differentiation methods yield higher regional identity while default methods produce broader cell type diversity.

This study shows that high NeuroD1 expression or its combination with other reprogramming factors improves neuronal conversion efficiency from glial cells.

This study uses single-cell RNA sequencing to map the in vivo reprogramming trajectory from acinar cells to pluripotency in the pancreas and identifies conserved intermediate markers across tissues.

This study shows that CEP83 deficiency in hiPSCs disrupts intermediate mesoderm differentiation, leading to impaired kidney progenitor formation and increased LPM marker expression.

This study shows Nek2 regulates neural differentiation by controlling metabolism, specifically preventing HIF1 stabilization to enable oxidative phosphorylation.

This study generates functional bone marrow from mouse ESCs in rat embryos using blastocyst complementation, demonstrating the ability to produce all hematopoietic cell lineages.

This study traces the evolutionary origin of OCT4/POU5 functions in supporting pluripotency across vertebrates using sequence analysis and functional studies in mouse ESCs.