Advanced Fluorescence Microscopy Techniques

Synaptic and Somatic Targeting of ArcLight, a Genetically Encoded Voltage Indicator

This study evaluates subcellular targeting of ArcLight, a GEVI, using different targeting sequences to enhance signal localization without affecting voltage sensitivity.

Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor dynamics probing lineage-determining function

The paper uses automated live-cell single-molecule tracking to study transcription factor dynamics in mouse enteroid monolayers, revealing contrasting diffusive behaviors that correlate with differentiation stages.

High-speed three-dimensional random access scanning with SPARCLS

This paper introduces SPARCLS, a high-speed 3D random-access scanning method achieving 340 kHz point rate, and demonstrates its application in imaging synaptic events in mammalian and zebrafish models.

Quantification of the effect of hemodynamic occlusion in two-photon imaging

This study quantifies hemodynamic occlusion effects in two-photon imaging and finds they can rival activity sensor signals and show spatial and behavioral variability.

GESIAP3.0: Sensor-based Image Analysis Program for Transmission Visualization In Vivo

GESIAP3.0 enables precise quantitative analysis of neuromodulatory synaptic transmission in behaving animals by correcting for tissue movement and optimizing synaptic information extraction.

Absorbing molecules make both abdomen and back transparent in live mice

This study reproduces a technique using absorbing molecules to achieve in vivo tissue transparency in live mice, identifies key experimental factors for reproducibility, and observes a new transparency effect in the back.

Reduced non-specific binding of super-resolution DNA-PAINT markers by Shielded DNA-PAINT labeling protocols

The paper introduces Shielded DNA-PAINT, a method to reduce non-specific DNA-PAINT probe binding in cells, achieving a 5-fold reduction in non-specific events in the nucleus.

Physics-Informed Ellipsoidal Coordinate Encoding Implicit Neural Representation for high-resolution volumetric wide-field microscopy

This paper introduces PIECE-INR, a physics-informed method for high-fidelity 3D fluorescence data reconstruction in wide-field microscopy, achieving state-of-the-art results without requiring ground truth data.

Impact of photobleaching of fluorescent proteins on FRET measurements under two-photon excitation

This study investigates how photobleaching affects FRET measurements under two-photon excitation and provides guidelines for reliable FRET experiments.

3D-Aligner: An advanced computational tool designed to correct image distortion in expansion microscopy for precise 3D reconstitution and quantitative analysis

3D-Aligner corrects image drift in expansion microscopy to enable accurate 3D reconstruction and quantitative analysis of biological structures.

The 3D point spread function can be autoencoded in latent space

This paper shows that the 3D point spread function of a fluorescent emitter can be directly accessed in the latent space of an autoencoder trained on sample images without z-reference data.

Innovative Strategies for Enhancing Signal-to-Noise Ratio in Single-molecule Imaging: The Influence of Molecular Motion

This study shows that molecular motion enhances signal-to-noise ratio in single-molecule fluorescence imaging and proposes a method to increase the upper concentration limit of fluorophore-tagged biomolecules.

Vortex light field microscopy: 3D spectral single-molecule imaging with a twist

This paper introduces vortex light field microscopy, achieving 3D spectral single-molecule imaging with 25 nm spatial and 3 nm spectral precision.

Reusable Microfluidic Chambers for Single-Molecule Microscopy

This paper demonstrates a method to reset and reuse single-molecule microfluidic chambers using photocleavable biotin and UV light, enabling at least 10 cycles without surface degradation.

Exploring transient states of PAmKate to enable improved cryogenic single-molecule imaging

This study investigates the photophysical properties of PAmKate to enhance cryogenic single-molecule imaging by improving emitter localization rates through a 2-color bleaching process.

NanoPlex: a universal strategy for fluorescence microscopy multiplexing using nanobodies with erasable signals

NanoPlex enables multiplex fluorescence microscopy using nanobodies with erasable signals, achieving up to 21 targets in 3D confocal imaging.

T-scope V4: miniaturized microscope for optogenetic tagging in freely behaving animals

The paper introduces T-scope V4, a miniaturized microscope that enables simultaneous neuronal activity imaging and optogenetic manipulation in freely moving mice by preventing biological crosstalk.

Targeted Photoactivatable Green-Emitting BODIPY Based on Directed Photooxidation Induced Activation and its Application to Live Dynamic Super-Resolution Microscopy

This paper develops a green-emitting photoactivatable BODIPY dye with a new photoactivation mechanism and demonstrates its use in live-cell super-resolution microscopy.

LiveLattice: Real-time visualization of tilted light-sheet microscopy data using a memory-efficient transformation algorithm

This paper introduces WH-Transform, a GPU-accelerated algorithm for real-time preprocessing of tilted light-sheet microscopy data, enabling fast visualization and analysis of large datasets.

Homogeneous large field-of-view and compact iSCAT-TIRF setup for dynamic single molecule measurements

The paper introduces a compact iSCAT-TIRF setup for dynamic single molecule measurements with a large field-of-view and uniform illumination.

Multiscale Light Field Microscopy Platform for Multi-purpose Dynamic Volumetric Bioimaging

This paper introduces a multiscale light field microscopy platform for fast 3D imaging across biological scales, demonstrating its use in whole-brain activity, calcium dynamics, and subcellular protein dynamics.

Cell-type-specific Labeling of Endogenous Proteins Using the Split GFP System in Drosophila

The paper presents a method to label endogenous proteins in a cell-type-specific manner using the split GFP system in Drosophila, demonstrating its application with the Ten-m gene.

FASER: A TOOL TO SIMULATE PSF DISTORTIONS IN STEDMICROSCOPY

Faser simulates excitation PSFs in STED microscopy to explore imaging performance and beam properties.

Development of AI-assisted microscopy frameworks through realistic simulation in pySTED

This paper develops a simulation platform, pySTED, to aid in creating AI-assisted super-resolution microscopy techniques by generating realistic data for training and testing AI models.

Sidelobe suppressed Bessel beams for one-photon light-sheet microscopy

This paper introduces a sidelobe suppressed Bessel beam (SSBB) for one-photon light-sheet microscopy, showing improved contrast and reduced need for deconvolution.

High Refractive Index Imaging Buffer for Dual Color 3D SMLM Imaging of Thick Samples

This paper develops a high refractive index imaging buffer to improve 3D SMLM imaging depth in thick samples by reducing RI mismatch and enhancing image quality.

T-CLEARE: A Pilot Community-Driven Tissue-Clearing Protocol Repository

The paper introduces T-CLEARE, a community-driven repository for tissue-clearing protocols to address replication challenges and improve protocol sharing and evaluation.

Parameter-free clear image deconvolution (CLID) technique for single-frame live-cell super-resolution imaging

This paper introduces CLID techniques for single-frame live-cell super-resolution imaging, achieving 65 nm resolution without complex parameters.

MINFLUX Reveals Dynein Stepping in Live Neurons

This study uses MINFLUX to track dynein movement in live neurons, revealing 8 nm steps and rapid direction reversals, suggesting a single ATP-dependent stepping mechanism.

The use of thermostable fluorescent proteins for live imaging in Sulfolobus acidocaldarius

The paper identifies a thermostable fluorescent protein, TGP, for live imaging in Sulfolobus acidocaldarius, enabling protein localization at high temperatures previously unattainable with mesophilic proteins.