Advanced Fluorescence Microscopy Techniques

Light-gated Integrator for Highlighting Kinase Activity in Living Cells

This paper introduces KINACT, a light-gated tool that converts kinase activity into permanent fluorescent marks for analyzing kinase dynamics and signaling mechanisms.

In Vivo Optical Clearing of Mammalian Brain

This study shows that modifying the optical properties of a mammalian brain can enhance deep imaging without affecting native physiology.

Serial Two-Photon Tomography Imaging of the whole marmoset brain for neuroanatomical analyses

The paper describes the implementation of serial two-photon tomography for whole marmoset brain imaging and introduces auxiliary histological techniques to enhance data utility.

iGlu3Fast and iGlu3Slow, improved fluorescent reporters for detection of spontaneous glutamate activity in the brain

The paper introduces two improved fluorescent glutamate sensors, iGlu3Fast and iGlu3Slow, with distinct kinetic properties for imaging glutamate activity in the brain.

Scalable projected Light Sheet Microscopy for high-resolution imaging of living and cleared samples

This paper introduces a cost-effective, scalable light sheet microscopy framework for high-resolution imaging of living and cleared samples, demonstrating its application on various biological samples.

Open-hardware solutions for light sheet microscopy specimen chambers

This paper introduces open-hardware solutions for light sheet microscopy specimen chambers using 3D printing, silicone injection moulding, and FEP-foil thermoforming to improve flexibility, sealing, and contamination prevention.

Ultraplex microscopy: versatile highly-multiplexed molecular labeling and imaging across scale and resolution

This paper introduces ultraplex microscopy, a method that enables highly multiplexed molecular labeling and imaging across different resolutions and modalities.

ClearScope: a fully integrated light sheet theta microscope for sub-cellular resolution imaging without lateral size constraints

The paper introduces ClearScope, a fully integrated light sheet theta microscope for sub-cellular resolution imaging of large specimens without lateral size constraints, demonstrating its use on mouse and human brain samples.

A method to increase the imaging efficiency of tiling light sheet microscopy using scanning non-coaxial beam arrays synchronized with regional virtual confocal slits

The paper introduces a method to enhance tiling light sheet microscopy efficiency using non-coaxial beam arrays and virtual confocal slits, showing improved imaging efficiency and practical robustness.

Optical constraints on two-photon voltage imaging

The paper compares one-photon and two-photon voltage imaging, finding that 2P requires much higher illumination power and faces tradeoffs between noise and tissue damage.

The eduWOSM: a benchtop advanced microscope for education and research

The paper introduces the eduWOSM, an open-source microscope for education and research, and benchmarks its performance with three imaging projects.

EVE is an open modular data analysis software for event-based localization microscopy

EVE is a software for analyzing event-based SMLM data, offering modular tools for detection, localization, and post-processing to fully utilize EBS capabilities.

Mirror-assisted light-sheet microscopy: a simple upgrade to enable bi-directional sample excitation

This paper introduces mirror-assisted light-sheet microscopy to improve imaging of obstructed regions in zebrafish brains.

3D live imaging and phenotyping of the subcellular cytotoxicity in cancer immunotherapy using event-triggered Bessel oblique plane microscopy

This study uses EBOPM to analyze subcellular events in CAR-T cells during immunotherapy, identifying key parameters correlated with cytotoxic function.

Comparative analysis of new, mScarlet-based red fluorescent tags in Caenorhabditis elegans

The paper compares the brightness and maturation time of new red fluorescent tags in C. elegans and finds mScarlet-I3 outperforms older versions and is comparable to GFP.

Rapid, biochemical tagging of cellular activity history in vivo

This paper introduces CaST, a method to biochemically tag cells with elevated Ca2+ in vivo, and demonstrates its use in tagging prefrontal cortex neurons activated by psilocybin in mice.

A posterior approach to correct for focal plane offsets in lattice light sheet structured illumination microscopy

This paper introduces a method to detect and correct axial offset between excitation and detection focal planes in latticeSIM to reduce image artifacts.

Cross-correlation increases sampling in diffusion-based super-resolution optical fluctuation imaging

This paper demonstrates that cross-correlation improves sampling and reduces aliasing in diffusion-based super-resolution optical fluctuation imaging for nanoscale dynamics and structure analysis.

Adapting and optimizing GCaMP8f for use in C. elegans

The paper optimizes GCaMP8f for use in C. elegans, showing it is brighter, faster, and less disruptive than GCaMP7f for measuring muscle and neuronal activity.

A fluorescent biosensor for the visualization of agmatine

The paper develops a fluorescent biosensor (AGMsen) to visualize agmatine dynamics in cells, demonstrating its functionality across neuronal cultures.

Mechanically Sheared Axially Swept Light-Sheet Microscopy

The paper introduces a new image acquisition method for light-sheet microscopes that improves spatial context placement and reduces post-processing needs, achieving higher field of view and resolution.

Novel Approach for Refractive Index Matching in Calcified Tissues

This paper introduces a modified BABB clearing protocol to improve imaging of calcified tissues by addressing their opacity and rigidity.

Non-Degenerate Two-Photon Imaging of Deep Rodent Cortex using Indocyanine Green in the water absorption window

This paper demonstrates non-degenerate two-photon imaging in rodent cortex using indocyanine green in the water absorption window, enabling deeper vascular imaging.

PUFFFIN: A novel, ultra-bright, customisable, single plasmid system for labelling cell neighbourhoods

PUFFFIN labels cell neighbourhoods using a fluorescent protein fusion, enabling tracking of cell interactions and profiling non-cell-autonomous responses in mammalian cells.

A Novel 3D Imaging Approach for Quantification of GLUT4 Levels across the Intact Myocardium

The paper introduces a 3D optical mesoscopy method to quantify GLUT4 levels in intact mouse hearts and shows gender-dependent expression changes with high fat diet.

PEPCy: Photostable fluoromodules for live cell, super-resolution microscopy of surface proteins

The paper introduces PEPCy, photostable fluoromodules that enhance cyanine dye photostability for live-cell super-resolution microscopy of surface proteins.

Simultaneous dual-color calcium imaging in freely-behaving mice

This paper introduces a dual-channel miniscope for simultaneous two-wavelength calcium imaging in freely behaving mice, showing spatial coding changes over time.

Self-quenched fluorophore-DNA labels for super-resolution fluorescence microscopy

The paper introduces self-quenched fluorophore-DNA labels to reduce background signal in super-resolution microscopy, improving imaging performance in STED and SMLM.

High-speed two-photon microscopy with adaptive line-excitation

This paper introduces a high-speed two-photon microscope using adaptive line-excitation to image neural activity in mouse cortex, increasing imaging speed and reducing laser power.

Improved Validation of Protein Interactions usingBicistronic BiFC (Bi2FC)

The paper introduces an improved BiFC method for validating protein interactions in plants by incorporating bicistronic expression and self-cleaving peptides to better assess expression and interaction status.