Advanced Fluorescence Microscopy Techniques

Towards optical measurements of membrane potential values in Bacillus subtilis using fluorescence lifetime

This paper introduces a fluorescence lifetime-based method to measure membrane potential in Bacillus subtilis, achieving single-cell resolution and quantification of MP changes under chemical perturbations.

Minimally Invasive DNA-Mediated Photostabilization for Extended Single-Molecule and Super-resolution Imaging

This paper develops a DNA-mediated photostabilization method for single-molecule and super-resolution imaging, showing improved performance at lower additive concentrations and enabling continuous replenishment of photostabilizers.

Molecular dynamics guided engineering of Aequorea victoria Green Fluorescent Protein chromophore interactions generates a brighter variant with improved photobleaching resistance

The paper engineers a brighter and more photostable variant of avGFP by modifying chromophore interactions, resulting in improved fluorescence and resistance to photobleaching.

Simulation-Guided Exploration of PAINT Parameter Space for Accurate Molecular Quantification

This paper develops a simulation-guided framework to optimize PAINT parameters for accurate molecular quantification by analyzing probe kinetics and imaging conditions.

Chromophore charge-state switching through copper-dependent homodimerisation of an engineered green fluorescent protein.

This paper engineers a green fluorescent protein that changes its chromophore charge state through copper-dependent homodimerisation, resulting in a 30-fold fluorescence emission ratio change.

Directionality of two-photon absorption in representative fluorescent proteins

The paper measures two-photon absorptivity tensors of fluorescent proteins to understand their directionality and provides insights for biosensor development.

Precise calcium-to-spike inference using biophysical generative models

The paper develops biophysically-inspired models to improve spike inference from calcium imaging by accounting for kinetic features of fluorescent indicators.

Cytosolic Enhanced Dark Epac-Based FRET Sensors for Intracellular cAMP detection in live cells via FLIM

The paper presents and characterizes mutations that improve cytosolic localization in FLIM-based cAMP FRET sensors while maintaining performance.

Ranking Single Fluorescent Protein Based Calcium Biosensor Performance by Molecular Dynamics Simulations

The paper uses molecular dynamics simulations to evaluate and rank the performance of single fluorescent protein-based calcium biosensors based on water distribution and hydrogen bond changes.

Obtaining super-resolved images at the mesoscale through Super-Resolution Radial Fluctuations

This paper demonstrates a method to achieve high-resolution imaging over a large field of view using SRRF and Mesolens, achieving 446.3 nm resolution with minimal error.

Exploration of mScarlet for development of a red lifetime sensor for calcium imaging

This study explores mScarlet-based red fluorescent proteins for developing a calcium indicator with fluorescence lifetime contrast, but finds that lifetime contrast is lost in mammalian cells compared to bacterial lysates.

MINFLUX fluorescence nanoscopy in biological tissue

This study demonstrates MINFLUX fluorescence nanoscopy's ability to achieve sub-10 nm resolution for protein localization in thick mouse brain tissue sections.

A Genetically Encoded Fluorescent Biosensor for Intracellular Measurement of Malonyl-CoA

This paper develops a genetically encoded fluorescent biosensor, Malibu, to measure intracellular malonyl-CoA dynamics in single cells, showing increased responses to fatty acid biosynthesis inhibitors.

A high-resolution microscopy system for biological studies of cold-adapted species under physiological conditions

This paper develops a high-resolution microscopy method for imaging live biological samples at sub-zero temperatures to study cold-adapted species under physiological conditions.

A comparison of super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium

This study compares super-resolution microscopy techniques for imaging tightly packed microcolonies of an obligate intracellular bacterium, finding 3D-STED best for axial resolution and differences in bacterial shape across cell lines.

All-optical mapping of Ca2+ transport and homeostasis in dendrites

The paper develops an all-optical system to measure calcium transport properties in dendrites, finding a mean length constant of ~5.8 m and a half-life of ~1.7 s.

PISA: versatile microscope for 3D single molecule light sheet imaging

PISA enables 3D single molecule light sheet imaging of coverslip-mounted samples with high-throughput capabilities and improved sensitivity for biochemical assays.

Multi-camera Simultaneous Total Internal Reflection and Interference Reflection Microscopy

The paper describes a modified multi-color TIRF microscope that enables simultaneous IRM and single molecule TIRF imaging of microtubules and EB1 proteins.

Hydrogel encapsulation of a designed fluorescent protein biosensor for continuous measurements of sub-100 nanomolar nicotine

This paper develops a hydrogel-based biosensor for continuous nicotine measurement in human biofluids, achieving detection at sub-100 nM levels with high stability and sensitivity.

Innervation density governs crosstalk of GPCR-based norepinephrine and dopamine sensors

The paper shows that high innervation density of cross-reacting transmitters causes non-specific activation of GPCR-based sensors, requiring specific innervation silencing for accurate interpretation.

Accelerated protein retention expansion microscopy using microwave radiation

This paper introduces a microwave-assisted expansion microscopy protocol that significantly reduces processing time while maintaining resolution and expansion efficiency across different organisms.

Super-Resolution Imaging of Fast Morphological Dynamics of Neurons in Behaving Animals

The paper develops a super-resolution microscopy technique to image neuronal morphology in behaving animals, revealing fast spinule dynamics and PSD-95 cluster correlations.

Long-term single-molecule tracking in living cells using weak-affinity protein labeling

This paper introduces a labeling method for long-term single-molecule tracking in living cells and demonstrates its application to study EGFR dynamics.

A novel rhodopsin-based voltage indicator for simultaneous two-photon optical recording with GCaMP in vivo

The paper introduces a novel rhodopsin-based voltage indicator, 2Photron, enabling simultaneous two-photon recording of spiking and subthreshold voltage with GCaMP in awake mice.

Long-term optical imaging of the spinal cord in awake, behaving animals

This study develops a method to image the spinal cord in awake, behaving mice over long periods, enabling monitoring of neural activity and behavior.

Fast and long-term super-resolution imaging of ER nano-structural dynamics in living cells using a neural network

This paper enables long-term super-resolution imaging of ER dynamics in living cells using low irradiation and neural network-based image restoration.

One step 4x and 12x 3D-ExM: robust super-resolution microscopy in cell biology

This study introduces 4x and 12x 3D-ExM methods for super-resolution microscopy, achieving nanometer-scale resolution for visualizing cellular structures.

Single Objective Light Sheet Microscopy allows high-resolution in vivo brain imaging of Drosophila.

This paper introduces a single objective light-sheet microscope for high-resolution in vivo brain imaging in Drosophila, achieving improved resolution and reduced photobleaching and phototoxicity.

Long axial-range double-helix point spread functions for 3D volumetric super-resolution imaging

This paper introduces long axial-range double-helix PSFs for 3D super-resolution imaging, enabling stitching-free imaging of whole mammalian cells with improved speed and resolution.

Fast and light efficient remote focusing for volumetric voltage imaging

The paper introduces FLIPR, a remote focusing technique that improves light efficiency and enables high-speed volumetric voltage imaging at 500 volumes per second.