Cellular transport and secretion

The paper shows that conserved arginine residues in synaptotagmin 1's C2B domain regulate fusion pore expansion by interacting with membranes during neurotransmitter release.

This study shows clathrin is a strong membrane curvature sensor, with its sensitivity amplified by adaptor proteins, highlighting the role of protein networks in curvature sensing.

This study shows that AP2 regulates Tkv internalization and recycling via Rab11 to control synaptic growth in Drosophila.

The paper models the dynamic interactions of Arf1 and GBF1 during COPI vesicle formation using FRAP data and simulations, revealing independent membrane association and distinct kinetics.

The paper shows that retromer complex dysfunction leads to mistargeting of secretory cargo and accumulation of amyloid precursor protein in enlarged endosomes, linking retromer defects to neurodegenerative disease progression.

The paper provides the first structural characterization of the yeast peroxisomal docking complex Pex14p/Pex17p, revealing its subunit organization and potential role in import pore formation.

The paper investigates how acetylcholine receptor activation in Retzius neurons affects vesicle redistribution through changes in Ca2+ levels, cellular stiffness, and optical phase difference.

The paper shows Munc13-1 directly binds SNAP25 to initiate SNARE complex assembly, using FRET and optical tweezer techniques.

This study shows that overexpressing an open form of syntaxin can rescue exocytosis defects in multiple C. elegans mutants, suggesting a general mechanism to enhance synaptic transmission.

The paper studies synaptic vesicle dynamics at mouse hippocampal synapses, showing rapid and reversible recruitment of vesicles to release sites after action potentials.

This study tracks inhibitory synaptic vesicles in real-time, revealing unique dynamics and exocytosis properties compared to excitatory vesicles.

The paper uses TIRF microscopy to study vesicle transport on synaptic ribbons in retinal bipolar cells, showing rapid vesicle mobilization and replenishment compared to conventional synapses.

The paper identifies non-neuronal Synaptobrevin (Syb) as essential for glial function in Drosophila motoneurons, showing that disrupting Syb leads to nerve disintegration and paralysis.

The paper shows that post-tetanic potentiation lowers the energy barrier for synaptic vesicle fusion independently of Synaptotagmin-1, enhancing release rates through additive mechanisms.

The paper shows that Syntaxin 7-marked synaptic vesicles are preferentially mobilized during high-frequency stimulation, requiring Ca2+ and actin.

The paper determines the structure and interactions of AP2 and clathrin during clathrin-coated vesicle formation using cryo-EM and tomography.

This study shows that Alix is required for activity-dependent bulk endocytosis at brain synapses, and its absence reduces seizure severity in mice.

This study identifies how complexin suppresses spontaneous exocytosis by binding the membrane-proximal regions of VAMP2 and SNAP25.

The paper shows that cytosolic Na+ accelerates synaptic vesicle endocytosis during high-frequency firing without affecting Ca2+ influx or exocytosis.

The paper shows that Flower and PIP2 interact to control activity-dependent bulk endocytosis through a positive feedback loop.

This study shows that synaptic vesicle retrieval after single-vesicle fusion is not dependent on dynamin, despite a reduction in overall neurotransmission and vesicle numbers.

This study identifies a RalGPS-Rala-exocyst-Rab11 pathway that regulates Pvr trafficking and signaling in Drosophila lymph glands, maintaining hematopoietic homeostasis.

This study characterizes the distribution and localization of PI5P4K and PI5P4Kβ in mouse and human brains, showing distinct expression patterns in different brain regions and cell types.

This study investigates the role of synaptotagmin 1's C2A domain in asynchronous neurotransmitter release using two distinct mutations, showing that Ca2+ binding by C2A does not inhibit asynchronous release.

This study identifies retrograde transport defects in MUNC18-1 null neurons as the cause of abnormal Golgi morphology and neurodegeneration.

This study shows that α-synuclein facilitates clathrin assembly during synaptic vesicle endocytosis by increasing clathrin patch size and curvature.

This study shows Drosophila Syt7 negatively regulates synaptic vesicle fusion and replenishment in a dosage-dependent manner, challenging the two Ca2+ sensor model of neurotransmitter release.

The paper identifies a novel activity-dependent mechanism involving Hrs and KIF13A for axonal transport of ESCRT machinery to facilitate synaptic vesicle protein degradation.

This paper develops optogenetic systems to precisely control nanoscale membrane curvature in live cells using engineered BAR domain proteins.