Discover, rate, and verify biology preprints.

Detection of CRISPR-Cas-induced mutations in Daphnia

This study compares T7EI and FA methods for detecting CRISPR-induced indels in Daphnia magna, finding FA more reliable for accurate mutation detection.

Zinc excess promotes lysosome remodeling by activating HLH-30/TFEB through the action of the high zinc sensor HIZR-1.

The paper identifies a zinc-regulated genetic pathway in C. elegans that activates lysosome remodeling through HIZR-1 and HLH-30/TFEB to enhance zinc detoxification.

Synthetic analogue of adrenocorticotropic hormone, ACTH(4-7)PGP delays neurological manifestations in diseases of mucopolysaccharidosis III spectrum by reducing neuroinflammation and rescuing neurotransmission, synaptogenesis, and axonal demyelination

The paper tests a synthetic peptide ACTH(4-7)PGP in MPS III mouse models, showing it improves synaptic function, reduces neuroinflammation, and extends lifespan.

T2T Pangenome Reveals a 3.3kb Structural Variation Driving the De Novo Evolution of a Subspecies-Specific NLR Gene in Rice

The paper identifies a 3.3 kb structural variation in rice that drives the de novo evolution of a subspecies-specific NLR gene, enhancing viral immunity.

Cruciform-forming AT/TA repeats are acted upon by structure selective endonucleases and Rad51 prior to repositioning to the nuclear periphery for repair

The paper shows that AT/TA repeats form DNA structures that are processed by nucleases and repositioned to the nuclear periphery for repair.

Signatures of sex ratio distortion in humans

The paper identifies a 2:1 male offspring ratio in a human family, suggesting potential segregation distortion caused by a distorting Y-chromosome.

Type 2 diabetes amplifies environmental Pb toxicity and intergenerational risk

This study shows that type 2 diabetes increases susceptibility to lead toxicity, leading to organ damage and intergenerational health risks.

Genome sequence and efficient CRISPR/Cas9 gene editing in the solanacea specialist pest Tetranychus evansi

This paper presents a high-quality genome assembly and CRISPR/Cas9 gene editing system for Tetranychus evansi, enabling studies of pesticide resistance and host adaptation.

Division state reveals hidden genetic regulation during T cell activation and identifies immune disease-linked gene programmes

The paper uses division-resolved single-cell transcriptomics to study T cell activation and identifies gene programmes linked to immune disease by analyzing variant effects on cell division states.

Hematological and genetic basis of clinical heterogeneity of HemoglobinE/β-thalassemia

The paper investigates the hematological and genetic factors contributing to clinical variability in HbE/β-thalassemia patients, identifying HbF and HbE levels and specific SNPs as modifiers of disease severity.

Life, the universe, and everything for $42: ultra-low pass sequencing of maize for genotyping, mapping, and pedigree analysis

The paper presents an economical ultra-low pass sequencing method for genotyping, mapping, and pedigree analysis in maize using skim sequencing.

A novel proliferative candidate genes panel for idiopathic pulmonary fibrosis: insights from integrated bulk and single-cell RNA sequencing

This study identifies a proliferative gene module in IPF using bulk and single-cell RNA-seq, linking it to cell cycle and oncogenic pathways, suggesting potential therapeutic targets.

Sry-modified laboratory rat lines to study sex-chromosome effects underlying sex differences in physiology and disease: Four Core Genotypes and more

The paper develops rat models with modified sex chromosomes to study sex differences in physiology and disease by comparing XX vs. XY and varying X/Y chromosome numbers.

Introgression from the wild relative Manihot glaziovii on cassava (M. esculenta) chromosome 1 exhibits segregation distortion and no direct effect on dry matter

The paper analyzes a cassava introgression from M. glaziovii, finding no direct effect on dry matter but segregation distortion and minor effects on vigor.

(Epi-)Genomic Data in the German TwinLife Study: TwinSNPs and TECS Cohort Profiles

The paper describes the curation and initial analysis of molecular genetic and epigenetic data from the TwinLife study, highlighting associations between polygenic scores and attrition, and stability of epigenetic clocks in saliva.

TRU-PE: A Universal, Trackable Prime Editor Toolkit for Robust Single- and Multi-Locus Genome Engineering

TRU-PE improves prime editing efficiency and scalability for genome engineering in human cells, enabling multi-locus edits and a platform for studying genetic disorders.

Enhanced muscle uptake of chemically optimized miR-23b antisense oligonucleotides as lead compounds for Myotonic Dystrophy type 1

This study develops chemically optimized miR-23b antimiRs that enhance muscle uptake and restore MBNL1 function in DM1 models, showing improved efficacy and safety.

Precise generation of bystander-free mouse models with ABE9-SpRY.

This paper introduces ABE9-SpRY, an improved adenine base editor that generates precise point mutations in mouse embryos with high efficiency and reduced off-target effects.

Recombination and the role of pseudo-overdominance in polyploid evolution

The paper simulates evolution in diploid and autotetraploid populations to show that pseudo-overdominance is more common in polyploids due to reduced recombination and increased masking of deleterious mutations.

Landrace and bred accessions of allotetraploid sour cherry (Prunus cerasus L.) reveal variation in subgenome dosage and subgenome expression bias

The paper examines subgenome dynamics in sour cherry, finding expression bias and homoeologous exchange events across different accessions and developmental stages.