CRISPR screen

This paper develops a CRISPR-guided transposon system for DSB-free DNA integration in human cells, improving efficiency and reducing off-target effects.

This paper introduces a one-step genome editing method for high-efficiency homozygous tagging of endogenous proteins with auxin-inducible degron in human cells, achieving >80% efficiency in A431 cells and >50% in H9 ES cells.

This study investigates chromosome loss in CRISPR-Cas9-engineered T cells and shows a modified manufacturing process reduces it while preserving editing efficacy.

This study demonstrates the use of protecting oligos to enhance crRNA stability and potency for in vivo genome editing via AAV co-delivery in mice.

This study evaluates KRASG12D inhibition in PDAC models, showing variable efficacy in 2D cultures but enhanced response in 3D cultures and xenografts, with immunological modulation contributing to tumor regression.

The paper assesses the impact of CRISPR perturbations on vascular endothelial cell functions at CAD loci, identifying variants that significantly affect endothelial phenotypes.

The paper introduces a composite gene delivery system called MAJESTIC for efficient and stable engineering of therapeutic immune cells, showing improved cell viability and transgene expression compared to existing methods.

This paper introduces a molecular glue approach using pomalidomide to control the half-life of CRISPR-based technologies, enabling precise regulation of their activity and therapeutic potential.

This paper develops a CRISPR-based RNA editing tool to restore Dendra2 fluorescence in neurons by editing specific nucleotides in the mRNA, enabling visualization of RNA-protein interactions.

The paper shows degenerated CRISPRs encode regulatory RNAs that autoregulate Cas protein expression, balancing immunity and host fitness costs.

This study investigates the role of UBE3A in Dup15q syndrome using CRISPR-corrected patient-derived neurons, showing UBE3A overexpression is necessary for most neuronal deficits but not synaptic phenotypes.

This paper introduces a toolkit for transgene integration in hiPSCs using a safe-harbour locus, enabling controlled transgene configurations and efficient cellular engineering.

The paper uses CRISPR tools to mimic cocaine-induced bidirectional regulation of Nab2 and Egr3 in specific NAc MSN subtypes.

MINORg generates minimal gRNA sets to cover multiple targets across multiple genomes, reducing workload for pangenomic screens.

This study evaluates the safety and efficacy of in vivo CRISPR gene editing targeting HSV-1 in patients with herpes stromal keratitis, finding no detectable off-target effects or adverse events over 18 months.

This paper designs an incoherent merger network in E. coli to achieve a ratiometric gene expression response that is linear, tunable, and robust to cellular perturbations.

This study compares CRISPR off-target detection tools using ex vivo edited CD34+ HSPCs, finding that HiFi Cas9 reduces off-target sites and some methods outperform others in sensitivity and specificity.

The paper introduces the Pathfinder plasmid toolkit to test genetic part compatibility in newly isolated bacteria, demonstrating its use in engineering Orbaceae strains to study their colonization of Drosophila gut.

The paper screens CRISPR interference mutants in Synechocystis sp. PCC 6803 across trophic conditions, revealing tradeoffs between growth rate and robustness and identifying condition-specific gene functions.

The paper develops DeepCas13, a deep learning model to predict CRISPR-Cas13d gRNA on-target activity and off-target viability effects, improving accuracy for RNA editing applications.

This study uses activity-based CRISPR scanning to identify allosteric sites in DNMT1 and UHRF1, revealing gain-of-function mutations that enhance DNA methylation activity.

CriSNPr provides a webserver for designing and querying gRNAs for CRISPR-based diagnostics using multiple Cas systems and includes a curated database of gRNAs for human and SARS-CoV-2 variants.

This paper shows that reducing DNA targeting activity in CRISPR systems improves genome editing efficiency in bacteria by enabling RecA-mediated repair.

This paper introduces REVeRT, a dual AAV vector system using mRNA trans-splicing for efficient gene delivery and editing, showing improved reconstitution and therapeutic potential in a mouse model.

This paper describes a three-day educational program teaching high school students genome editing through a base editing experiment in E. coli, including background lectures and ethics discussions.

ShrinkCRISPR analyzes CRISPR-Cas9 screen data to detect differential gene fitness effects while accounting for variation across cell populations and replicates.

The paper performs a genome-wide CRISPR/Cas9 knock-out screen to identify genes involved in phagocytosis, validates key complexes, and maps their functional roles in human cells.

This study optimizes genomewide CRISPR screens using AsCas12a and multi-guide arrays, finding that three guides per gene construct provide optimal performance.

PerturbSci-Kinetics enables analysis of transcriptome kinetics through single-cell RNA profiling of CRISPR screens, revealing key regulators in miRNA and mitochondrial RNA processing.

This study identifies HTLV-1 splice sites in gene vectors causing splicing perturbations in transgenic human cells and shows that modifying these sites can enhance genomic safety without compromising therapeutic efficacy.