flow cytometry

Characterization of the pulmonary immune response induced by a highly protective tuberculosis vaccine using latent and acute infection mouse models

This paper evaluates a tuberculosis vaccine regimen in mouse models, finding it highly effective in preventing reactivation and acute infection by enhancing specific immune cell populations.

A tailored in vivo CRISPR screen identifies BAP1 as a potent tumor suppressor of soft tissue sarcoma

The paper identifies BAP1 as a tumor suppressor in soft tissue sarcoma using an in vivo CRISPR screen and shows that PLK1 inhibition can suppress tumor growth in BAP1-deficient models.

Optimized In Vitro Expansion of Vδ1+ γδT Cells from Nonhuman Primate Peripheral Blood

This study optimizes in vitro expansion of Vδ1+ γδT cells from nonhuman primate PBMCs using CD3 or PHA stimulation with cytokines and phospho-vitamin C, achieving high-fold expansions.

Human hepatic stellate cells orchestrate the accumulation and function of CD103+ tissue-resident CD8+ T-cells in liver fibrosis

Intracellular autofluorescence enables the isolation of viable, functional human muscle reserve cells with distinct Pax7 levels and stem cell states.

This paper identifies human muscle reserve cells (MuRC) subpopulations using intracellular autofluorescence, finding that AFHigh cells are enriched in Pax7High cells with delayed activation and slower proliferation.

Splendid isolation with migration: Diversity dynamics of South American mammals

The paper models how immigration of African and North American mammals affected South American mammal diversity, finding increased overall richness but declining native diversity over time.

Label-Free In-Line Characterization of Immune Cell Culture using Quantitative Phase Imaging

This paper introduces qOBM for non-destructive, label-free monitoring of T cell cultures, showing strong correlation with traditional assays and potential for improving cell therapy development.

Cell state transitions drive the evolution of disease progression in B-lymphoblastic leukemia

This paper develops a mathematical model of cell state transitions in B-ALL to correlate with BCR::ABL1 status, MRD, and relapse risk using flow cytometry data.

L-Phenylalanine is a metabolic checkpoint of human Th2 cells

This study identifies L-phenylalanine as a metabolic checkpoint regulating Th2 cell development, energy metabolism, and function in allergy.

Inflammatory profiles of transdiagnostic symptom dimensions in healthy females

This study links transdiagnostic symptom dimensions in healthy females to specific inflammatory profiles using Bayesian regression and PCA, showing associations between social withdrawal and monocyte counts, and anxious-depression with stress-induced inflammation.

Human Interleukin-4-Dependent Facilitation of Human IgG Production in PBL-NOG-hIL-4-Tg mice

This study shows that NOG-hIL-4-Tg mice support human IgG production through IL-4-dependent B cell maintenance and class switching, with varying subclass responses and tertiary lymphoid structures.

Optimized protocol for processing murine tumor-bearing lung tissue for flow cytometry and single cell RNA-sequencing

The paper presents a protocol for isolating high-quality single-cell suspensions from murine lung tissue for scRNA-seq and flow cytometry.

PD-1 blockade during T cell priming enhances long-term protection against metastatic tumors by epigenetically tuning T cell exhaustion

This study shows PD-1 blockade during T cell priming promotes an intermediate-exhausted CD8 T cell state that supports durable anti-tumor immunity after surgery and metastatic challenge, better than effector or memory-like T cells.

Pre-treatment naive T cells are associated with severe irAE following PD-1/CTLA4 checkpoint blockade for melanoma

This study identifies pre-treatment naive CD4+ T cell abundance as a predictor of severe irAEs in patients receiving anti-PD-1/CTLA4 checkpoint inhibitor therapy for melanoma.

Imaging flow cytometry enables label-free cell sorting of morphological variants from unculturable bacterial populations.

This paper introduces a label-free method using imaging flow cytometry to sort bacterial morphotypes from unculturable populations, achieving >90% purity for downstream analyses.

Autoimmunity and H. pylori infection cooperatively promote epithelial tumorigenic differentiation and systemic immune modulation

This study shows that autoimmunity and H. pylori infection together promote gastric tumorigenesis and immune suppression in mice.

Systemic immune alterations in a murine experimental model of osteoarthritis

This study profiles systemic immune changes in a murine OA model, finding reduced MHCII on macrophages and DCs, increased tolerogenic DCs, and shifts in T cell composition.

Circulating Chlamydia trachomatis-specific T Cell Immunity Reflects Widespread Exposure in South African Adolescents and Young Women

This study examines circulating T cell immunity to Chlamydia trachomatis in South African adolescents and young women, finding that immune response magnitude and quality correlate with infection clearance and mucosal inflammation.

Infection Of Rhesus Macaques With Onyong-nyong Virus UVIR-O804 Recapitulates Key Aspects of Human Clinical Disease

This study infects rhesus macaques with a recent ONNV clinical isolate, demonstrating viremia, immune activation, and clinical disease features similar to human infection.

Minimal immune cell subset differences in a cohort of close contacts of tuberculosis index cases

This study examines immune cell differences in close contacts of TB patients based on QFT status, finding minimal differences among subgroups but clear immune changes in active TB patients.

Endothelial protein C receptor CD201 is a better marker than SCA1 to identify mouse long-term reconstituting hematopoietic stem cells following septic challenge

This paper shows that CD201 is a more reliable marker than SCA1 for identifying long-term reconstituting hematopoietic stem cells in mice after septic challenge.

Microplastics cross the murine intestine and induce inflammatory cell death after phagocytosis by human monocytes and neutrophils

Congenital CMV infection drives oligoclonal expansion of cytotoxic γδ T cells from early fetal progenitors

The paper shows congenital CMV infection leads to oligoclonal expansion of cytotoxic γδ T cells with fetal-like TCR features in neonates.

Non-selective beta blockers reduce bystander CD8+ T cell activation in decompensated liver cirrhosis

This study shows that non-selective beta blockers reduce bystander CD8+ T cell activation in decompensated liver cirrhosis without affecting antigen-specific responses.

GPR15 and CD38 define a subset of peripheral blood pathogenic effector Th2 cells associated with active eosinophilic esophagitis

The paper identifies GPR15+ CD38+ peripheral blood Th2 cells associated with active eosinophilic esophagitis, showing potential as a non-invasive biomarker and suggesting new disease mechanisms.

Inter-Instrument Quantification of Fluorescence Read-out Signal Using DNA Origami Calibrator Beads

This study uses DNA origami calibrator beads to calibrate and compare the sensitivity of five flow cytometers for detecting dimly fluorescent nano-sized particles.

Comparative impact of porcine reproductive and respiratory virus and swine influenza A virus infections on respiratory lymph nodes B cells and macrophages.

This study compares the effects of PRRSV-1 and swine influenza A virus on respiratory lymph node B cells and macrophages, identifying PRRSV-specific immune evasion mechanisms.

Intrathymic progenitor cell transplantation restores T cell development through ILC3-TEC crosstalk

The paper shows that intrathymic HSPC transplantation restores T cell development via ILC3-TEC interactions in a ZAP-70-deficient mouse model.

Deep single-cell immune and signaling profiles predict long term therapy response in chronic myeloid leukemia within hours

The paper uses mass cytometry to analyze immune and signaling profiles in CML patients within hours of TKI treatment to predict 12-month treatment response.

Multi-omics identification of activated T cells and spatial PD-1/PD-L1 signaling as biomarkers of diabetic foot ulcer healing

The paper identifies T cell activation and PD-1/PD-L1 signaling as biomarkers for diabetic foot ulcer healing using multi-omics approaches across multiple cohorts.