flow cytometry

Phenotypic dynamics and behaviors of neutrophils during barrier challenge

This study tracks neutrophil dynamics during barrier challenge, revealing environment-dependent phenotypic diversity and identifying a novel CD101-low, PD-L1+ neutrophil subset with antimicrobial functions.

T-bet expressing Tr1 cells driven by dietary signals dominate the small intestinal immune landscape

This study identifies a dominant population of IL-10-producing, T-bet-expressing CD4+ Tr1 cells in the small intestine that are shaped by dietary antigens rather than the microbiota.

A high-throughput flow cytometry assay to evaluate CatSper-mediated Ca2+ influx triggered by high-K+/high-pH stimulation in mouse sperm

This paper develops a high-throughput flow cytometry assay to screen for CatSper inhibitors in mouse sperm by measuring Ca2+ influx under high-K+/high-pH conditions.

Flow cytometry protocols, relative genome size and ploidy levels for 1104 species of non-apomictic angiosperms from the Eastern Alps - a community resource based on the screening of 45,000 samples

The paper provides genome size and ploidy level data for 1104 non-apomictic angiosperm species from the Eastern Alps using flow cytometry on silica-dried samples.

Post-Assay Photogelation Enables Flow-Cytometric Sorting and Recovery of Bacterial Cocultures from Microfluidic Droplets

This paper introduces post-assay photogelation to enable flow-cytometric sorting of bacterial cocultures from microfluidic droplets, achieving high-purity sorting and demonstrating its application in microbial community analysis.

Microparticle-Enabled Single Cell Multiparameter Electronic Immunophenotyping for Selective Electroporation

The paper introduces ME-SPICy, a microfluidic platform for targeted electroporation of selected cell subpopulations using antibody-conjugated microparticles and impedance detection, achieving 5-fold enrichment of lymphocytes.

Mucosal innate immune activation as the trigger to Prevotella species-induced arthritis in genetically resistant mice

This study shows that Prevotella intestinalis induces arthritis in resistant mice by activating intestinal immune cells and promoting Th17 differentiation through IL-6.

High-throughput heterospheroid-based screening identifies drugs that reprogram tumor-associated macrophages

This study identifies two compounds that reprogram tumor-associated macrophages from M2-like to M1-like antitumor phenotype using a heterospheroid-based screening platform.

Tracking karyotype dynamics by flow cytometry reveals de novo chromosome duplications in laboratory cultures of Macrostomum lignano

The paper tracks karyotype dynamics in Macrostomum lignano using flow cytometry, showing spontaneous de novo chromosome duplications in lab cultures and proposing a method to control karyotypes.

Preventing surgery induced immune suppression and metastases by inhibiting PI3K-gamma signalling in Myeloid-Derived Suppressor Cells.

The paper identifies PI3K-gamma signaling as a key pathway in surgery-induced MDSCs that suppresses NK cells and promotes metastases, showing that targeting this pathway can reduce postoperative metastases.

A flow cytometry-based assay to quantify the binding of transmembrane ligands to their cognate receptors using fluorescent virus-like particles

This paper introduces a flow cytometry-based assay using fluorescent virus-like particles to quantify transmembrane ligand-receptor binding, showing it can study patient-derived variants and enable single-cell analysis of multiple interactions.

Scalable generation of pure CD103⁺ cDC1 from iDC1 cultures

This paper develops a scalable method to generate pure CD103+ cDC1 from mouse bone marrow cultures, achieving high purity and yield for immunological studies.

Repeated administration of cannabidiol decreases splenic lymphocyte numbers in rats: involvement of CB2 receptors

This study shows that repeated CBD administration in rats reduces splenic lymphocyte numbers via CB2 receptors, similar to its effects in peripheral blood.

Holotomography-driven learning for in-silico staining of single cells in flow cytometry avoiding co-registration

This paper introduces a Holotomography-driven learning method for virtual staining in flow cytometry without requiring co-registration, enabling label-free single-cell analysis.

Automated Image-Based Cell Sorting by Targeted Photopolymerization

This paper introduces an automated image-based cell sorting method that enriches activated T cells from blood with high throughput and label-free morphology-based classification.

Antigen-specific CD8 T cells are generated and reactivated in the bone marrow following viral brain infections and impact the bone marrow niche

This study shows that virus-specific CD8 T cells are generated and reactivated in the bone marrow after brain viral infections, leading to changes in the bone marrow niche.

CellTrap: A Microfluidic Platform Enabling Cell-Cell Interactions at Variable Effector to Target Ratios

CellTrap enables long-term, single-cell analysis of immune-cancer interactions by controlling effector-to-target ratios, showing enhanced immune activity with higher ratios.

Compositional Analysis of Mouse Sperm Subpopulations During Capacitation In Vitro

This study uses single-cell flow cytometry and statistical modeling to analyze how mouse sperm subpopulations change during capacitation in vitro, revealing nonlinear interactions between bicarbonate and calcium.

A multilevel hierarchical framework for quantification of experimental heterogeneity

This paper introduces a Bayesian hierarchical framework to quantify both inter- and intra-experimental heterogeneity in dynamical processes using differential equations, demonstrating its robustness and flexibility.

CyTOF-based profiling of circulating tumor cells predicts aggressiveness and therapy response in SCLC liquid biopsies at a personalized level

This study uses CyTOF to profile circulating tumor cells in SCLC patients, identifying subtype-specific markers and treatment response correlations.

Neutrophils matter: New clinical insights on their role in the progression of metastatic breast cancer

This study investigates the clinical impact of immunosuppressive low-density neutrophils in metastatic breast cancer, finding they correlate with faster disease progression and reduced survival.

Stable characteristics of intrapopulation heterogeneity in virus-specific Th1 cells during chronic viral challenge infection

The paper examines T-bet expression stability in virus-specific Th1 cells during chronic infection, finding that quantitative differences persist and some cells retain Tfh-like features.

Temporal single-cell profiling uncovers age-associated delays in immune resolution to respiratory viral infection

This study identifies age-associated delays in immune resolution to respiratory viral infection by analyzing single-cell RNA sequencing and flow cytometry data from young and aged mice.

Dedicated nanoparticle flow cytometry for single extracellular vesicle phenotyping: Performance of the CytoFLEX Nano

The paper evaluates the CytoFLEX Nano flow cytometer's performance for analyzing extracellular vesicles in complex biofluids.

Opposing Functions of Distinct Regulatory T Cell Subsets in Colorectal Cancer

The paper identifies two distinct regulatory T cell subsets in colorectal cancer with opposing roles in tumor growth and prognosis.

Aiolos restricts the generation of antigen-inexperienced, virtual memory CD8+ T cells

This study identifies Aiolos as a negative regulator of antigen-inexperienced, virtual memory CD8+ T cells, showing increased TVM cell numbers and function in its absence.

The altered T cell landscape in Systemic Sclerosis patients is characterized by dysfunctional type 1 immunity.

This study identifies dysfunctional type 1 immunity in SSc patients through altered T cell subsets and reduced IFN-γ production.

High-Dimensional Single-Cell Analysis Reveals Coordinated Age-Dependent Neuroinflammatory Microglia-T cell Circuits in the Brain

This study identifies age-related neuroinflammatory microglia-T cell circuits in a mouse model of vascular dementia using multi-modal high-dimensional analysis.

Vitamin D differentially modulates effector and regulatory T-cell migration across the blood-brain barrier

This study shows that vitamin D reduces effector T-cell migration across the blood-brain barrier while preserving regulatory T-cell entry, potentially benefiting multiple sclerosis patients.

The Changing Immune Landscape of Innate-like T Cells and Innate Cells Throughout Life

This study uses spectral flow cytometry to analyze age-related changes in innate and innate-like T cells, identifying age-associated phenotypic shifts in immune cell subsets.