flow cytometry

Deep Phenomics of Pattern Recognition Receptor Agonist Specific Activation of Human Blood

The paper uses deep phenomics to compare immune cell activation in whole blood and PBMCs, finding neutrophils and NK cells as primary sources of CCL4 production in WB and PBMCs, respectively.

Exploring the neuroimmune cellular landscape in the skin of subjects with fibromyalgia.

This study examines skin neuroimmune changes in fibromyalgia patients, identifying novel dermal alterations and correlations between immune cell populations and nerve fiber density.

Alterations in Circulating T-Cell Subsets with Gut-Homing/Residency Phenotypes Predict HIV-1 Status and Subclinical Atherosclerosis

The paper identifies specific T-cell subsets with gut-homing/residency markers that predict HIV status and subclinical atherosclerosis in ART-treated individuals.

Temporal Dynamics of Antigen-Specific T Cell Expansion in Primary SARS-CoV-2 Infection

The paper quantifies T cell response dynamics during primary SARS-CoV-2 infection, showing clone-intrinsic properties drive clonal kinetics and providing insights for vaccine design.

Phospho 394Y LCK flow-cytometry readout predicts dasatinib sensitivity in paediatric T-cell acute lymphoblastic leukaemia

The paper identifies phospho-LCK (pY394) as a biomarker for dasatinib sensitivity in paediatric T-ALL using flow-cytometry, showing strong correlation with drug response.

Atypical memory B cells contribute to heterogeneous vaccine responses in ocrelizumab-treated patients with multiple sclerosis

The paper examines how B-cell subsets in ocrelizumab-treated multiple sclerosis patients influence vaccine responses to SARS-CoV-2, identifying specific B-cell populations associated with immune response variability.

Isolation of rat and human hepatic cholangiocytes using a peptide derived from a conserved domain of enterobacteria BamA/TamA-like proteins

The paper identifies a peptide that binds to cholangiocytes and uses it to isolate human and rat liver epithelial cells, revealing functional differences between small and large cholangiocytes.

Targeting Siglec-10/α3β1 Integrin Interactions Enhances Macrophage-Mediated Phagocytosis of Pancreatic Cancer

This paper identifies Siglec-10/α3β1 integrin interactions as a mechanism suppressing macrophage phagocytosis in PDAC and shows that targeting these interactions enhances anti-tumor immunity.

A flow cytometric approach to identifying the relative abundance and functional capacities of hemocyte subsets in the American cockroach, Periplaneta americana (L.)

The paper uses flow cytometry to identify and characterize hemocyte subsets in the American cockroach, finding that granular hemocytes perform key immune functions like phagocytosis and melanization.

Divergent effects of cytomegalovirus and rheumatoid arthritis on senescent CD4+ T cells

This study compares the effects of cytomegalovirus and rheumatoid arthritis on senescent CD4+ T cells using mass cytometry, finding distinct differentiation and functional profiles.

Changes in peripheral blood leukocyte composition precede development of heart-reactive autoantibodies in patients hospitalised for acute heart failure

Beta-2-microglobulin stimulates neutrophil phagocytosis of bacteria and apoptotic cells

This study shows that beta-2-microglobulin enhances neutrophil phagocytosis of bacteria and apoptotic cells, contributing to innate immunity and tissue homeostasis.

In Vitro Maturation of Bone Marrow-Derived Dendritic Cells via STING Activation for T Cell Priming

This study generates and matures bone marrow-derived dendritic cells in vitro using STING activation to enhance CD8 T cell priming.

Ligand Discrimination in Immune Cells: Signal Processing Insights into Immune Dysfunction in ER+ Breast Cancer

The paper measures ligand discrimination errors and molecular noise in immune cells of ER+ breast cancer patients compared to healthy controls using a communication model.

Dietary iron deficiency impairs effector function of memory T cells following influenza infection

This study shows that dietary iron deficiency impairs memory T cell function after influenza infection, specifically reducing IFN-gamma production without recovery through dendritic cell co-culture.

A 37-color spectral flow cytometry panel to characterize phenotype and cytokine expression in human intestinal T cells

The paper develops a 37-color spectral flow cytometry panel to analyze T cell phenotypes and cytokine expression in human intestinal samples, with applications in inflammatory bowel disease research.

Longitudinal Multi-omic Immune Profiling Reveals Age-Related Immune Cell Dynamics in Healthy Adults

This study uses multi-omic profiling to analyze immune cell dynamics in healthy adults over time, finding age-related transcriptional changes in T cells and impaired memory B cell responses in older adults.

Acetoacetate suppresses colon cancer via an MR1-MAIT axis

This study shows that acetoacetate suppresses colon cancer by expanding and activating MAIT cells via the MR1 receptor, offering a potential immunotherapy approach.

Low-dose infusion of a superagonistic anti-CD28 monoclonal antibody ameliorates inflammation after acute myocardial infarction in pigs

This study shows low-dose CD28-SA infusion reduces inflammation and promotes wound healing in pigs after myocardial infarction by increasing Treg cells and reducing TNF-producing cells.

AutoSpectral improves spectral flow cytometry accuracy through optimised spectral unmixing and autofluorescence-matching at the cellular level

AutoSpectral enhances spectral flow cytometry accuracy by optimizing unmixing matrices and matching autofluorescence at the cellular level, significantly reducing errors in complex datasets.

Immuno-functionomics reveals geographical variation and a role for TLR8 in mRNA vaccine responses

The study uses spectral flow cytometry to analyze immune function across populations, revealing geographical differences and TLR8's role in mRNA vaccine responses.

CCR2-driven monocyte recruitment is protective against radiotherapy-induced intestinal toxicity

The paper shows that CCR2-driven monocyte recruitment reduces radiotherapy-induced intestinal toxicity by enhancing IL-17 production.

Expanded adaptive NKG2C+ NK cells exhibit potent ADCC and functional responses against HBV-infected hepatoma cell lines

This study expands adaptive NKG2C+ NK cells from HBV-infected donors, showing enhanced ADCC and anti-hepatoma activity.

Hemin Inhibits the Activation of STING in Macrophages by Inducing HO-1, Promoting Endometriosis Development

This study shows hemin induces HO-1 in macrophages, inhibiting STING pathway activation and promoting endometriosis development.

Divergent role of CD8 T cells with distinct metabolic phenotypes during curative radio-immunotherapy in hot versus cold tumors

This study examines how CD8 T cells with different metabolic profiles contribute to tumor cure in hot versus cold murine tumors using radio-immunotherapy.

Mapping of CD8 T-cell recognition to latent EBV infection and neuroantigens reveals HLA-specific depletion of T-cell responses in multiple sclerosis

The paper identifies HLA-specific depletion of CD8 T-cell responses to EBV and neuroantigens in MS patients, revealing immunophenotypic differences between MS and healthy donors.

Development of antigen-dextramers for detection and evaluation of CAR T cells

This paper develops antigen-dextramers to stain and functionally assess CAR T cells, showing they enable both detection and activation analysis with adjustable antigen density.

Targeting platelet GPVI or GPIIb/IIIa only minimally affect acute inflammation and cardiac repair in experimental permanent myocardial infarction

Automatic computational classification of bone marrow cells for B cell pediatric leukemia using UMAP

The paper develops an AI algorithm using UMAP to classify bone marrow cells in B-ALL patients, improving disease prognosis and therapy decisions by identifying key subpopulations.

Relationship between SARC-F score and Peripheral Blood Lymphocytes in Juvenile Idiopathic Arthritis: An Observational Pilot Study

This study examines the relationship between SARC-F score and immune profiles in JIA patients, finding differences in T cell subpopulations and suggesting a link between exercise and immune status.