DNA Repair Mechanisms

The paper shows Rad53 binds to gene promoters under replication stress, influencing gene expression and coordinating replication with transcription.

The paper identifies FANCC as an anti-CO gene conserved from vertebrates to plants and shows it regulates meiotic recombination with FANCE and FANCF.

This study measures DNA replication speeds in human cells using single-cell sequencing and finds replication accelerates during S-phase, limited by transcriptional activity early in the phase.

The paper directly determines meiotic crossovers in fission yeast S. pombe, confirming the absence of crossover interference and revealing co-variation in CO numbers across chromosomes.

This paper shows that Rad51-mediated interhomolog recombination in budding yeast meiosis is inhibited during Phase 1 to prevent competition with Dmc1, and is promoted by the checkpoint and Pif1 helicase.

This study compares two budding yeast disome systems, finding similar mechanisms of chromosome instability but conflicting results on chromosome size changes and higher instability frequency in the ChrV system.

This study shows Rad5 has opposite roles in DNA damage tolerance: promoting error-free bypass via PCNA ubiquitination and enabling error-prone TLS by recruiting Rev1.

The paper uses Pu-seq to map DNA polymerase usage in human cells, revealing Polε synthesizes the leading strand and Pol contributes to lagging strand synthesis, and links transcription to polymerase uncoupling and chromosomal instability.

The paper identifies two motifs in the Swi2-Swi5 complex that enable cell-type-specific localization to recombination enhancers and stimulate Rad51-driven gene conversion in fission yeast.

The paper induces unscheduled DNA replication in G1-phase budding yeast, showing it leads to genome instability through head-to-tail replication fork collisions during subsequent S-phase.

This study shows that the TOPOVIBL-REC114 interaction is essential for the efficiency and timing of meiotic DNA double-strand breaks in mice.

This paper investigates how nonhomologous tails influence heteroduplex rejection and mismatch correction during SSA in yeast, showing that tails stimulate rejection and affect correction mechanisms.

The paper shows that overexpression of WHI7 bypasses the need for Mec1 and Rad53 in Saccharomyces cerevisiae, revealing a transcription-independent role in delaying G1/S transition during replication stress.

This study investigates how aging and sperm depletion affect DNA break formation and repair in C. elegans germlines, finding that repair defects are age-related and independent of sperm presence.

This study identifies genetic interactions linking the PriA/PriB DNA replication restart pathway to double-strand DNA break repair in E. coli using transposon insertion sequencing and targeted experiments.

This study identifies BRCA1-IRIS as a critical isoform for ICL repair, distinct from BRCA1-p220, by interacting with FANCD2 and promoting its ubiquitylation.

This study shows that transcript RNA facilitates DSB repair in human cells through NHEJ and MMEJ, promoting repair or mediating RNA-templated repair based on sequence complementarity.

The paper identifies POLK's role in maintaining rRNA transcription by acting on ribosomal DNA during DNA damage, revealing its interaction with nucleolar proteins and dependence on PCNA ubiquitination and PARP1.

This study investigates how repeat length affects Marcal1-dependent single-strand annealing in Drosophila, finding that 500-2000 bp is needed for maximum efficiency and that Marcal1 dynamics influence SSA rates.

This study identifies SOD1 as a key factor in mitotic DNA damage response, showing it mediates mitotic delay and DNA repair, with distinct pathways for repair or progression.

This study uses Duplex Sequencing to detect DNA replication errors and mutations in E. coli, identifying in vivo mutations in mutL and mutT strains and revealing context effects and strand bias.

The paper identifies a second PCNA mutation, C148S, causing PARD, showing both variants have thermo-stability defects leading to reduced chromatin-bound PCNA and temperature-dependent phenotypes.

The paper identifies C1orf112 as a novel regulator of interstrand crosslinks that decreases FIGNL1-RAD51 interaction, impairing homology-directed repair.

This study shows APE1 regulates the ATR-Chk1 DNA damage response by assembling biomolecular condensates in nucleoli, independent of its nuclease and redox functions.

This study identifies RNA binding proteins as novel drivers of homologous recombination deficiency in tumors, expanding the known genetic causes beyond BRCA1/2 mutations.

The paper identifies 160 genes that suppress DNA damage in human cells through CRISPR/Cas9 screens, highlighting GNB1L's role in ATR biogenesis and its link to neuropsychiatric disorders.

This study shows that Hrq1/RECQL4 regulates DNA intrastrand crosslink repair and its misregulation contributes to genomic instability in cancer.

This study shows DNA replication timing regulates oncogenic chromosomal translocations by affecting DSB ligation, independent of DSB frequency.

The paper develops a quantitative modeling framework to analyze DNA repair kinetics in yeast, finding gene size correlates with repair rates.

The paper determines the crystal structure of Vibrio cholerae (6-4) photolyase, revealing interactions with three cofactors and a potential DNA binding region.