Ion channel regulation and function

The N-terminal PAS domain directly regulates hERG channel gating in excised, inside-out patch-clamp fluorometry (PCF) recordings

This study investigates whether the PAS domain remains attached to hERG channels during gating using patch-clamp fluorometry, finding that PAS remains associated despite mechanical and conformational changes.

Dopaminergic amacrine cells express HCN channels in the developing and adult mouse retina

The paper identifies HCN channel expression and function in dopaminergic amacrine cells across mouse retinal development.

Ligand-Coupled Conformational Changes in a Cyclic Nucleotide-Gated Ion Channel Revealed by Time-Resolved Transition Metal Ion FRET

This study uses time-resolved tmFRET to analyze conformational changes and energetics in the SthK C-terminal region upon cAMP and cGMP binding, revealing large structural changes with cAMP and partial agonism with cGMP.

Biophysical Modeling and Experimental Analysis of the Dynamics of C. elegans Body-Wall Muscle Cells

This paper develops a biophysically detailed model of C. elegans body-wall muscle cells using experimental data and a simulation-based inference method to estimate parameters and validate the model's accuracy.

Versatile Functional Interaction between Electrically Silent KV Subunits and KV7 Potassium Channels

The paper shows that electrically silent KV subunits modulate KV7 channels' activity and biophysical properties, revealing versatile cross-family interactions in native cell types.

From resting state to inactivation: the dynamic journey of the inactivation peptide in Shaker Kv channels

The paper tracks the movement of the inactivation peptide in Shaker Kv channels during N-type inactivation using voltage clamp fluorometry and FRET techniques, revealing a biphasic mechanism involving release and pore blockage.

Sulfonylurea receptor coupled conductances alter the performance of two central pattern generating circuits in Cancer borealis

The paper investigates how sulfonylurea receptor (SUR) conductances affect two central pattern generating circuits in Cancer borealis, showing opposite effects on neuronal firing in different circuits.

A reinterpretation of the relationship between persistent and resurgent sodium currents

The paper investigates the role of resurgent (INaR) and persistent (INaP) sodium currents in regulating repetitive firing in Purkinje neurons, finding that INaP, not INaR, is critical for firing rate control.

Structure of the Ion Channel Kir7.1 and Implications for its Function in Normal and Pathophysiologic States

The paper presents the structure of Kir7.1, explains pathogenic mutations, and shows how a small molecule blocks the channel, affecting MC4R neurons and food intake.

Molecular and Cellular Context Influences SCN8A Variant Function

This study examines how alternative splicing and cellular context affect the functional properties of SCN8A variants associated with neurodevelopmental disorders.

Loss of Intracellular Fibroblast Growth Factor 14 (iFGF14) Increases the Excitability of Mature Hippocampal and Cortical Pyramidal Neurons

This study shows that loss of iFGF14 increases excitability of mature hippocampal and cortical pyramidal neurons by altering INaT voltage-dependence and action potential waveform.

Computational modelling of specific serotonin reuptake inhibitor inhibition on the TREK-2 channel

This study uses molecular dynamics to show how fluoxetine and escitalopram inhibit TREK-2 channels through different mechanisms involving the selectivity filter and lipid interactions.

Equivalent excitability through different sodium channels and implications for the analgesic efficacy of selective drugs

The paper shows that nociceptors can achieve equivalent excitability through different sodium channel subtypes, challenging the effectiveness of subtype-selective analgesic drugs.

Impact of extracellular current flow on action potential propagation in myelinated axons

This paper shows that extracellular conditions significantly affect action potential conduction velocity in myelinated axons by influencing axial current flow.

Impact on backpropagation of the spatial heterogeneity of sodium channel kinetics in the axon initial segment

The paper investigates how spatial variations in sodium channel kinetics along the axon initial segment affect backpropagation of action potentials during orthodromic and antidromic stimulation.

Mutant analysis of Kcng4b reveals how the different functional states of the voltage-gated potassium channel regulate ear development

The paper studies two mutations in the zebrafish Kcng4b gene, showing how they affect Kv channel function and lead to distinct developmental defects in ear development.

Altered neurological and neurobehavioral phenotypes in a mouse model of the recurrent KCNB1-p.R306C voltage-sensor variant

This paper generates a Kcnb1R306C mouse model to study the effects of a voltage-sensing mutation in KCNB1, finding altered neurological and neurobehavioral phenotypes.

Disease-causing Slack potassium channel mutations produce opposite effects on excitability of excitatory and inhibitory neurons

The paper investigates how disease-causing gain-of-function mutations in the Slack potassium channel affect excitability differently in excitatory and inhibitory neurons.

Analysis of the effect of the scorpion toxin AaH-II on action potential generation in the axon initial segment

The paper investigates how the scorpion toxin AaH-II affects action potential generation in the axon initial segment of mouse neurons using high-resolution imaging techniques.

Yeast Bax Inhibitor (Bxi1p/Ybh3p) is a Calcium Channel in E. coli

The paper shows that yeast Bxi1p/Ybh3p is a calcium channel in E. coli, and that gadolinium inhibits it, supporting its classification as a TMBIM family member.

Kinetics and functional consequences of BK Channels activation by N-type Ca2+ channels in the dendrite of mouse neocortical layer-5 pyramidal neurons

This study investigates the interaction between N-type Ca2+ channels and BK channels in mouse neocortical layer-5 pyramidal neurons, revealing their functional coupling and impact on AP shape and Ca2+ influx.

Upregulated GIRK2 counteracts ethanol-induced changes in excitability and respiration in human neurons

The paper investigates how upregulated GIRK2 affects ethanol-induced changes in human neurons, showing it counteracts excitability and respiration changes.

Structural basis for excitatory neuropeptide signaling

This study determines the structural basis of neuropeptide binding and gating in a DEG/ENaC channel using cryo-EM and mutagenesis.

Distinct cellular expression and subcellular localization of Kv2 voltage-gated K+ channel subtypes in dorsal root ganglion neurons conserved between mice and humans

The paper identifies the cellular and subcellular localization of Kv2.1 and Kv2.2 channels in DRG neurons, showing similarities between mice and humans.

A Kv2 inhibitor combination reveals native neuronal conductances consistent with Kv2/KvS heteromers

The paper uses two Kv2 inhibitors to distinguish Kv2/KvS heteromer conductances from Kv2-only channels in neurons.

Postinhibitory excitation in motoneurons can be facilitated by hyperpolarization-activated inward currents: a simulation study

This study uses computational modeling to show that hyperpolarization-activated inward currents (h-currents) can facilitate postinhibitory excitation in human motoneurons.

Regulation of Kv1.2 redox-sensitive gating by the transmembrane lectin LMAN2

This study identifies LMAN2 as a regulatory protein that modulates redox-sensitive gating of Kv1.2 channels by altering activation kinetics and redox sensitivity.

KCNQ2/3 regulates efferent mediated slow excitation of vestibular afferents in mammals

This study shows that KCNQ2/3 channels are targeted by mAChR activation in mammalian vestibular afferents, modulating slow excitation and discharge rates.

CaV2.1 α1 subunit motifs that control presynaptic CaV2.1 subtype abundance are distinct from CaV2.1 preference

The paper investigates motifs in CaV2.1 subunits that regulate presynaptic abundance and preference, finding distinct motifs control abundance versus preference.

The intracellular C-terminus confers compartment-specific targeting of voltage-gated Ca2+ channels

The paper identifies the C-terminus of voltage-gated Ca2+ channels as the determinant for their compartment-specific targeting in neurons.