Pluripotent Stem Cells Research

This study investigates how LIF signaling perturbations affect Sox2 transcriptional dynamics in mESCs, revealing that reduced Sox2 expression, not complete loss, drives pluripotency loss.

The paper uses human pluripotent stem cells to study the epiblast development transition, revealing a transcriptional clock mechanism that governs timing and directionality through gene regulatory networks.

This study generates and maintains hindbrain-like neural stem cells without xeno- or basic fibroblast growth factors using three chemicals, showing long-term stability and potential for disease modeling and therapy.

This study develops a method to efficiently generate human oligodendrocytes from induced pluripotent stem cells, which can form functional myelinating grafts in adult human brain tissue.

This paper introduces comBOs, a new human bone and lympho-myeloid bone marrow organoid model that includes multiple cell types and improves upon existing models for studying haematopoietic disorders.

This paper describes a modified viral transduction method to differentiate iPSCs into steroidogenic cells by first forming embryoid bodies before SF1 transduction.

This study shows that low-dose DMSO treatment improves hiPSC differentiation into kidney organoids by enhancing SIX2 expression and altering pluripotent gene expression.

The paper develops a SAMHD1 knockout iPSC model that enhances lentiviral transduction efficiency in myeloid cells, enabling better functional genomics studies in innate immunity and inflammatory diseases.

This study shows that cadherin switching during mesendoderm differentiation from human pluripotent stem cells is independent of cell fate commitment, with distinct signaling dependencies.

This study identifies H3K27ac as an epigenetic barrier to reprogramming-induced cell fate changes using an AI-based approach and shows that reducing H3K27ac improves reprogramming efficiency.

This study generates iPSC-derived hepatocytes from A1ATD donors and shows they recapitulate disease features and respond to drug treatments, offering a model for drug screening.

This study develops a feeder-free serum-coated system for culturing naive human pluripotent stem cells, maintaining their developmental potential and reducing contamination and mutations.

The paper identifies the Feret diameter as a reliable quality marker for brain organoids and shows mesenchymal cells negatively impact organoid formation.

This paper describes a human pluripotent stem cell-derived heart organoid that recreates coronary vascularization and sympathetic innervation with functional organization.

This study develops a method to generate human skeletal assembloids that recapitulate limb skeletal development and endochondral ossification with longitudinal growth.

This study improves the yield and subtype identity of hPSC-derived midbrain dopamine neurons through optimized WNT and FGF18 signaling modulation, enhancing their functional and transcriptional similarity to primary neurons.

This paper introduces two mouse embryoid models to study interactions between primitive endoderm and epiblast during early development, showing they replicate in vivo dynamics and cell fate decisions.

This study identifies histone modification fluctuations as a cause of PSC differentiation bias and presents a chemical chromatin restoration method to reverse it, restoring epiblast-like differentiation potential.

The paper identifies EHMT2's role in repressing totipotency-associated gene loci in mESCs by binding to LINE-1 elements and modulating gene expression during the mESC-to-2CLC transition.

This study establishes a method to derive and culture bovine extraembryonic endoderm cells (bXENs) using a chemical cocktail, revealing their role in maintaining stemness and supporting embryo development.

The paper introduces a method to generate spinal motor neurons with diverse axial identities in human spinal cord organoids, enhancing their utility for studying neural development and disease.

This study generates cardiac resident-like macrophages and cardiomyocytes from iPSCs to investigate their interactions in 2D and 3D settings, showing improved cardiomyocyte maturation and function when co-cultured.

This study develops a method to generate diverse GABAergic neurons from PSCs using TFs and signaling inhibition, showing distinct subtype compositions and disease relevance.

This study identifies LINE1 RNA's trans-regulatory roles in maintaining ESC identity through distinct mechanisms in differentiation and 2C-like cell processes.

The paper develops a cortical assembloid system to study human PVALB+ interneuron development and identifies schizophrenia-associated defects in their migration and molecular profiles.

The paper identifies a quiescent-like state with reduced nuclear size and transcriptional activity that enables efficient TF-mediated conversion of fibroblasts to neurons.

The paper uses multiomics analysis of 2D and 3D hiPSC-CMs under insulin resistance conditions to show metabolic and contractile dysfunction resembling diabetic cardiomyopathy.

This paper develops optimised human iPSC-derived hepatocytes (Opti-HEP) with improved liver function for metabolic disease modelling and toxicity studies, showing comparable functionality to primary human hepatocytes.

The paper evaluates four organoid protocols to recreate human brain cell diversity, identifies factors affecting variability, and introduces a scoring system to assess differentiation propensities.