Cellular transport and secretion

The paper investigates RILP knockdown effects in neurons, finding a neuron-specific off-target Golgi disruption that may not reflect RILP's role in endosomal transport.

The paper shows that cAMP signaling opens dilating synaptic fusion pores for releasing large proteins, while Ca2+ triggers narrower pores for smaller cargo.

The paper shows that yolk granule fusion and microtubule aster formation drive cortical granule translocation and exocytosis in zebrafish oocytes during maturation.

The paper reconstitutes core synaptic machinery to reveal DAG's role in vesicle priming and Ca2+-triggered release, showing DAG accelerates SNAREpin assembly and affects release dynamics.

The paper uses a multiomic approach to study Retromer's role in lysosomal health in human neuroglioma cells, revealing proteomic and transcriptomic changes linked to neurodegeneration.

The paper shows that MARCKS masks PIP2, arresting SNARE assembly and vesicle fusion, while PIP2 unmasking by Ca2+/CaM or PKC-epsilon rescues fusion and enhances synaptotagmin-1 activity.

This study identifies the phosphorylation-dependent interaction between Amph1 and endophilin A1 as essential for synaptic vesicle endocytosis.

The paper identifies GEFs and GAPs modulating Rab10 phosphorylation and abundance by LRRK2-G2019S in neurons, showing Crag acts as a GEF and plx as a conventional GAP.

The paper identifies deacidification of distal lysosomes in aged neurons as a mechanism driving synapse loss and shows that restoring acidification can reverse synaptic decline.

The paper introduces pOpsicle, an all-optical system for tracking synaptic vesicle recycling using pH-sensitive fluorescent proteins and optogenetic stimulation in C. elegans.

The paper investigates the relationship between synaptic vesicle endocytosis and docking, showing that a rapid recycling pathway preferentially docks vesicles from its origin.

The paper uses cryo-electron tomography to sequence events during synaptic vesicle exocytosis and identifies how tether formation and connector dissolution are regulated by stimulation and spontaneous fusion rate.

The paper investigates the fate of exophers in C. elegans, showing they are engulfed and degraded by hypodermal cells through a phagocytic process involving specific molecular factors.

This study investigates Arf6's role in angiogenesis by analyzing its impact on actin assembly and clathrin-mediated endocytosis in endothelial cells.

This study identifies Rab10 as essential for TrkB sorting and retrograde BDNF signaling in neurons, linking Rab10 to neuroprotection and neurodegeneration.

This study identifies synaptotagmin-7 as a Ca2+ sensor in splanchnic nerve terminals that regulates acetylcholine release and short-term presynaptic facilitation.

This study shows that SYT9 regulates spontaneous neurotransmitter release in striatal neurons by controlling substance P secretion, without triggering rapid synaptic vesicle exocytosis.

The paper performs a genome-wide CRISPR/Cas9 knock-out screen to identify genes involved in phagocytosis, validates key complexes, and maps their functional roles in human cells.

The paper shows that ATG9A forms distinct vesicle phases from synaptic vesicle proteins within synapsin condensates in fibroblasts and nerve terminals.

The paper shows that synaptic vesicle proteins selectively enter axons and are delivered to presynaptic membranes before being incorporated into vesicles, challenging the selective retention model.

This paper shows that phosphorylation of Syntaxin1 at Ser14 is critical for non-vesicular dopamine release and AMPH-induced behaviors in Drosophila.

This study identifies TMEM87A as a novel Golgi-resident cation channel, GolpHCat, which regulates pH homeostasis and is essential for brain function and memory.

The paper identifies an isoform-specific role of Cdc42b in regulating Exo70 and axon formation in neurons, distinct from the ubiquitous Cdc42u isoform.

The paper introduces nHU, a method to quantify binding affinities from cell extracts, showing agreement between in vitro and in-cell measurements.

The paper shows that SV2A-Syt1 nanoclusters limit dynamin-1 recruitment, thereby controlling the re-entry of Syt1 into recycling synaptic vesicles.

The paper develops an automated assay to analyze Golgi morphology and single-cell transcriptomes, revealing that late endosomal pathways compensate for Golgi disruption caused by USO1 knockdown.

The paper investigates how Ca2+ and chelators like ATP and EGTA regulate the cis- and trans-membrane interactions of synaptotagmin-1 using purified vesicles and liposomes.

The paper investigates the trafficking of three organelle markers in thalamocortical axons in vivo, finding differences in speed and behavior compared to in vitro studies.

The paper investigates whether Munc13 supports vesicle fusogenicity independently of active zone scaffolds and synaptic vesicle docking.

This study identifies Synaptotagmin-3 as a high-affinity Ca2+ sensor that accelerates synaptic vesicle replenishment and short-term synaptic plasticity.