Cellular transport and secretion

The paper shows that weakening of septate junctions in Drosophila epithelial cells triggers changes in adhesive and mechanical properties through ESCRT-III and Retromer pathways.

The paper identifies specific amino acid residues in the complexin N-terminus that differentially affect spontaneous and evoked neurotransmitter release in hippocampal neurons.

This study shows that the juxtamembrane linker of synaptotagmin 1 mediates liquid-liquid phase separation, which modulates Ca2+ binding and synaptic vesicle exocytosis.

The paper develops a quantitative model of vacuole fusion dynamics in plant guard cells to predict a dual role for HOPS in regulating SNARE complex formation and activity.

This paper introduces a live-cell imaging assay using TIRF microscopy to track particle uptake by clathrin-mediated endocytosis and analyzes how particle properties affect uptake efficiency.

The paper shows JNK signaling increases exocytosis in dying cells, promoting Wg secretion and cell-turnover in Drosophila wing development.

The paper investigates the role of the PXDLS/T-binding cleft in Ribeye's function at synaptic ribbons, showing that mutations in this region impair ribbon structure and synaptic transmission in zebrafish.

This study identifies mDia1/3 and Rho GTPase signaling as critical regulators of presynaptic actin dynamics and endocytosis.

The paper uses molecular phylogenetic analysis and homology modeling to show that the electrostatic membrane-binding surface of synaptotagmin-like proteins is highly conserved across vertebrates.

This study compares synaptotagmin-1 and synaptotagmin-7, finding that synaptotagmin-7 more effectively penetrates membranes and stabilizes fusion pores during exocytosis.

This study shows Tweek is essential for astrocyte phagocytosis and ER-PM contact formation during neuronal remodeling in Drosophila.

The paper shows PDZD8 promotes autophagy at ER-lysosome contact sites to regulate synaptogenesis through lipid transfer and lysosome maturation.

This paper identifies Synaptophysin as a chaperone that organizes 12 SNAREpins under each synaptic vesicle through its hexamer structure and VAMP2 dimer binding.

This study investigates how SYT1 variants affect synaptic vesicle exocytosis and clarifies that defective evoked exocytosis is the main pathogenic mechanism of SYT1-associated neurodevelopmental disorders.

This study identifies AP-3 and VPS41 as factors that promote high-frequency dopamine release by targeting VMAT2 to axons and producing specialized synaptic vesicles.

This study identifies Doc2 as the major Ca2+ sensor for asynchronous release and shows that syt7 supports vesicle docking, with combined effects on synaptic function.

The paper characterizes spatial and temporal expression patterns of VPS10P domain receptors in human cerebral organoids using single-cell RNA sequencing.

The paper develops a CRISPR toolkit for tissue-specific gene knockout in Drosophila motor neurons, muscles, and glial cells, revealing ESCRT's role in NMJ extracellular vesicle formation.

This study identifies Emp as a key regulator of apical membrane morphogenesis in Drosophila airways by mediating endocytosis of luminal cargo proteins.

The paper identifies key residues in Munc18-1 domain 3a that regulate the interaction with VAMP2, controlling nanoscale reorganization necessary for vesicular priming and secretion.

This study shows that tomosyn regulates DCV cargo levels but not exocytosis in neurons, suggesting a role in cargo production rather than release.

This study investigates the roles of complexin/CPX-1 and CAPS/UNC-31 in regulating excitatory and inhibitory synaptic release at C. elegans neuromuscular junctions, revealing distinct regulatory patterns and interactions.

The paper investigates the combined effects of two exocytosis-enhancing mutations in C. elegans and finds they disrupt excitatory-inhibitory balance and impair multiple behaviors.

This study identifies suppressors of RDGB function in Drosophila and links them to novel lipids and ubiquitination processes in retinal degeneration and neurodevelopmental diseases.

This study examines how disease mutations in SNAP25 alter the energy landscape of synaptic exocytosis by affecting SNARE interactions and fusion dynamics in neurons.

This study shows that deleting VPS50 in mice brains impairs synaptic function and behavior by disrupting V-ATPase pump localization and vesicle acidification.

This study identifies dAsap as a regulator of NMJ morphology and synaptic calcium homeostasis in Drosophila through Arf6-dependent signaling.

The paper identifies a novel phosphorylation of Rab29 at Ser185, mediated by PKC, which regulates its localization and lysosomal stress response in conjunction with LRRK2.

The paper uses cryo-electron tomography to study synaptic vesicle tethering and priming, revealing the role of Munc13 and SNAP25 in confining vesicles and guiding fusion.