Extracellular vesicles in disease

Expression of Arabidopsis Extracellular Vesicle Protein Markers in Nicotiana benthamiana Reveals Distinct Vesicle Subpopulations

The paper uses fluorescence microscopy to show that plant extracellular vesicles (EVs) are heterogeneous, with distinct markers like TET8 and PEN1 labeling different subpopulations.

High-throughput, multiplexed quantification, and sorting of single EVs at single-molecule level

This paper introduces BDEVS, a platform for high-throughput, multiplexed, and ultra-sensitive profiling of individual extracellular vesicles in plasma, demonstrating a 100x improvement in detecting EV subpopulations.

Control of physical and biochemical parameters influencing exogeneous cargo protein association to extracellular vesicles using lipid anchors enables high loading and effective intracellular delivery

This study identifies critical parameters for lipid-anchored protein loading into extracellular vesicles and demonstrates efficient intracellular delivery.

CD24 regulates the formation of ectosomes in B lymphocytes

This study identifies a novel pathway involving CD24, aSMase, PI3K/mTORC2/ROCK/actin that regulates bioactive ectosome formation in B lymphocytes.

Ribonuclease activity undermines immune sensing of naked extracellular RNA

This study shows that naked extracellular RNA can be pro-inflammatory and functionally active in immune cells when ribonuclease inhibitors are present, challenging the need for RNA encapsulation.

Vaginal bacteria-derived extracellular vesicles diffuse through human cervicovaginal mucus to enable bacterial signaling to upper female reproductive tract tissues

This study shows that vaginal bacteria-derived extracellular vesicles can diffuse through cervicovaginal mucus and interact with reproductive tract tissues, potentially contributing to disease and offering therapeutic potential.

Identification of HIV Tat and NF-κB binding proteins associated with semen-derived extracellular vesicles

The paper identifies host proteins in human semen-derived extracellular vesicles that interact with HIV Tat and NF-κB, suggesting they may repress HIV by targeting transcription factors.

Exercise-induced extracellular vesicles mediate apoptosis in human colon cancer cells in an exercise intensity-dependent manner

This study shows that exercise-induced extracellular vesicles reduce colon cancer cell viability and induce apoptosis, with higher intensity exercise producing stronger effects.

Utility of brain-derived extracellular vesicles from human umbilical cord blood to measure non-infectious neuroinflammation and functional iron deficiency

This study isolates and analyzes brain-derived extracellular vesicles from umbilical cord blood to detect non-infectious neuroinflammation and neonatal iron deficiency in infants.

Unveiling the functional heterogeneity of endogenous tissue extracellular vesicles in skeletal muscle through multi-omics

This study identifies functional differences between subpopulations of endogenous tissue-derived extracellular vesicles in skeletal muscle, showing distinct biological effects and potential applications in biomarker discovery and therapy.

Extracellular vesicle-derived miRNA-mediated cell-cell communication inference for single-cell transcriptomic data with miRTalk

miRTalk infers EV-derived miRNA-mediated cell-cell communication from scRNA-seq data, showing high accuracy in simulated and real datasets.

Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC-derived neurons

The paper validates EV isolation from multiple biofluids using orthogonal methods but finds L1CAM does not reliably enrich for neuron-derived EVs.

Membrane vesicles in freshwater ecosystems: Quantification by flow cytometry and interferometric microscopy

This study quantifies membrane vesicles in freshwater ecosystems using flow cytometry and interferometric microscopy, finding agreement in nanoparticle abundance and suggesting associations with virus particles or dense compounds.

Muscle-derived miR-200a-3p through light-intensity exercise may contribute to improve memory dysfunction in type 2 diabetic mice

This study shows light-intensity exercise increases miR-200a-3p in muscle exosomes and plasma, improving memory in T2DM mice, and mimicking exercise effects through miR-200a-3p treatment.

Rigorous process for isolation of gut-derived extracellular vesicles and the effect on latent HIV

This paper describes a method to isolate gut-derived extracellular vesicles (ColEVs) and shows that purified ColEVs can activate the HIV LTR promoter.

Manipulation of the Extracellular Vesicle Biogenesis Pathway in Paternal Somatic Cells Contributes to the Phenotypic Fate of the Next Generation

This study shows that manipulating the extracellular vesicle biogenesis pathway in male Drosophila somatic cells leads to sex-specific phenotypic changes in offspring.

Exosomal cargo genes as biomarkers and potential mediators of exosome-driven cell communication in Pleural Mesothelioma

The paper characterizes exosomal RNA cargo in pleural mesothelioma, identifies potential biomarkers, and explores their role in cell communication and prognosis.

Yersinia pestis actively inhibits the production of extracellular vesicles by human neutrophils

This study shows Yersinia pestis inhibits neutrophil extracellular vesicle production, reducing antimicrobial cargo and inflammatory properties.

Exploring the adhesion properties of extracellular vesicles for functional assays

This study investigates how extracellular vesicles adhere to different surfaces and shows that borosilicate glass coverslips can effectively immobilize EVs for imaging techniques.

Chronic contractile activity induced skeletal muscle-derived extracellular vesicles increase mitochondrial biogenesis in recipient myocytes via transmembrane or peripheral membrane proteins

Milk-derived extracellular vesicles mitigate NF-κB pathway and NLRP3 inflammasome formation in Long Evans neonates.

This study shows that milk-derived extracellular vesicles reduce NF-κB pathway and NLRP3 inflammasome activation in neonatal rats exposed to maternal high fat diet.

A Novel Aptamer-Based Approach for Lipoprotein Removal to Achieve Ultra-Pure Blood EV Isolation

This paper develops ApoFilter, an aptamer-based method to remove lipoproteins from blood, enabling ultra-pure extracellular vesicle isolation with high efficiency and minimal EV loss.

Large extracellular vesicles subsets and contents discrimination: the potential of morpho mechanical approaches at single vesicle level

This study uses morpho-mechanical analysis and machine learning to distinguish subpopulations of large extracellular vesicles based on their physical properties and mitochondrial content.

Designed Peptides as Affinity Ligands for Extracellular-Vesicle-based Cancer Biomarker Detection

The paper designs peptides as alternatives to antibodies for detecting protein biomarkers on extracellular vesicles, showing comparable affinity to antibodies.

Circulating extracellular vesicles can transport stress signals to the male germline

The paper shows that stress-induced changes in extracellular vesicles from mice can alter sperm and embryo transcriptomes, leading to metabolic issues in offspring.

A proposed workflow to analyze bacterial transcripts in RNAseq from blood extracellular vesicles of people with Multiple Sclerosis

The paper proposes a workflow to analyze bacterial transcripts in RNAseq data from blood extracellular vesicles of people with Multiple Sclerosis to improve taxonomic profiling accuracy.

AdmirePred: A method for predicting abundant miRNAs in Exosomes

This paper develops AdmirePred, a method to predict abundant miRNAs in exosomes using machine learning and hybrid approaches, achieving an AUC of 0.854.

Microfluidic Nano-Plasmonic Imaging Platform for Purification- and Label-Free Single Small Extracellular Vesicle Counting

This paper introduces a microfluidic platform for label-free single small extracellular vesicle counting in plasma without purification, using plasmonic nano-aperture imaging.

Small extracellular vesicles from young adipose-derived stem cells ameliorate age-related changes in the heart of old mice.

This study shows that young adipose-derived stem cell extracellular vesicles improve cardiac function and reduce aging-related damage in old mice.

Identification and proteomic profiling of CD90+ small EVs using a refined immunocapture separation approach targeting stromal-derived EV subpopulations in synovial fluid of arthritis patients

This paper develops an immunocapture method to isolate CD90+ stromal-derived EVs from arthritis synovial fluid and performs proteomic profiling to identify disease-relevant EV subpopulations and biomarkers.