N. W. Snyder

αKG-mediated carnitine synthesis promotes homologous recombination via histone acetylation

This study shows that αKG-mediated carnitine synthesis supports HR proficiency by promoting histone acetylation, which enhances DNA repair.

The chemotherapy-induced senescence-associated secretome promotes cell detachment and metastatic dissemination through metabolic reprogramming

This study shows that chemotherapy-induced senescence-associated secretome promotes metastasis in ovarian cancer by reprogramming metabolism and increasing cell detachment.

ATR promotes mTORC1 activity via de novo cholesterol synthesis

This study shows ATR enhances mTORC1 activity through cholesterol synthesis via LSS, independent of CHK1 and TSC, and this effect is amplified in p16-deficient cells.

Lactoylglutathione promotes inflammatory signaling in macrophages

This study shows that lactoylglutathione (LGSH) promotes inflammatory signaling in macrophages by increasing histone lactoylation and chromatin accessibility.

Trogocytosis of cancer-associated fibroblasts promotes pancreatic cancer growth and immune suppression via phospholipid scramblase anoctamin 6 (ANO6).

This study identifies ANO6-mediated trogocytosis as a mechanism by which CAFs transfer lipids to PDAC cells, promoting tumor growth and immune suppression.

De novo purine metabolism is a metabolic vulnerability of cancers with low p16 expression

This study identifies de novo purine metabolism as a vulnerability in cancers with low p16 expression, showing sensitivity to anti-folate drugs like methotrexate.

Acyl-CoA thioesterase-2 facilitates beta-oxidation in glycolytic skeletal muscle in a lipid supply dependent manner

This study shows ACOT2 regulates acyl-CoA levels in glycolytic muscle, promoting β-oxidation when lipid supply is low but causing lipid accumulation and poor glucose homeostasis when lipid supply is high.

Comparison of colorimetric, fluorometric, and liquid chromatography-mass spectrometry assays for acetyl-coenzyme A

The paper compares colorimetric, fluorometric, and LC-MS-based assays for measuring acetyl-CoA, finding that LC-MS/MS and LC-HRMS provide reliable results with internal standards, while colorimetric ELISA fails even with standards.

Dynamic protein deacetylation is a limited carbon source for acetyl-CoA-dependent metabolism

This study shows that dynamic protein deacetylation in Acly-/- MEFs contributes carbons to acetyl-CoA but only transiently supplies about 9% of cellular acetyl-CoA.

The carnitine shuttle links mitochondrial metabolism to histone acetylation and lipogenesis

This study identifies the carnitine shuttle and CrAT as a pathway for mitochondrial-to-cytosolic acetyl-CoA transfer, independent of ACLY, in cancer cells.

Genetic depletion of de novo coenzyme A biosynthesis exacerbates puromycin toxicity

The paper shows that reducing coenzyme A biosynthesis increases cancer cells' sensitivity to puromycin, a selection antibiotic used in research.

Coenzyme A governs proinflammatory macrophage metabolism

This study shows that exogenous CoA enhances TLR-driven proinflammatory and antimicrobial responses in macrophages by promoting oxidative metabolism and histone acetylation.

Ketones facilitate transcriptional resolution of secondary DNA structures in premature aging

This study shows that ketones like beta-hydroxybutyrate reduce premature aging features in Cockayne syndrome models by enhancing transcriptional readthrough of DNA secondary structures through histone acetylation.

Thymidine rescues ATR kinase inhibition induced deoxyuridine contamination in genomic DNA, cell death, and Type 1 interferon expression

This study shows that thymidine rescues ATR kinase inhibitor-induced deoxyuridine contamination, cell death, and Type 1 interferon expression in CD8+ T cells.

Persistent DNA damage rewires lipid metabolism and promotes histone hyperacetylation via MYS-1/Tip60.

This study shows persistent DNA damage alters lipid metabolism and histone acetylation via MYS-1/Tip60, leading to immune-like responses and age-related decline.

O-GlcNAc transferase regulates glioblastoma acetate metabolism via regulation of CDK5-dependent ACSS2 phosphorylation

This study identifies OGT's role in regulating GBM acetate metabolism through CDK5-dependent ACSS2 phosphorylation, showing that targeting this pathway inhibits tumor growth.

Primary saturation of α,β-unsaturated carbonyl containing fatty acids does not abolish electrophilicity

This study investigates the electrophilicity of saturated oxo-fatty acids and finds that mono-saturation retains electrophilicity, while double saturation abolishes it, implicating PTGR1 in their metabolism.

The deacylase SIRT5 supports melanoma viability by regulating chromatin dynamics

This study identifies SIRT5 as essential for melanoma cell survival and tumor formation, showing it regulates chromatin dynamics and gene expression through histone modifications.

Quantitative sub-cellular acyl-CoA analysis reveals distinct nuclear regulation

The paper introduces SILEC-SF, a method for sub-cellular metabolomic analysis, and finds distinct nuclear acyl-CoA profiles with notable propionyl-CoA enrichment and a new metabolic pathway for histone propionylation.

Direct anabolic three carbon metabolism via propionate to a six carbon metabolite occurs in human heart and in vivo across mouse tissues

The paper confirms the in vivo synthesis of a six-carbon metabolite from propionate in human heart and mouse tissues through non-oxidative pathways.

ATM inhibition drives metabolic adaptation via induction of macropinocytosis

This study shows that ATM inhibition promotes cancer cell survival by increasing macropinocytosis, which can be suppressed by BCAA supplementation.

Quantification of lactoyl-CoA (lactyl-CoA) by liquid chromatography mass spectrometry in mammalian cells and tissues

This paper quantifies lactoyl-CoA in mammalian cells and tissues using LC-HRMS and provides a biosynthetic method for isotopically labeled lactoyl-CoA.

Cumulus cell acetyl-CoA metabolism from acetate is associated with maternal age but only partially with oocyte maturity

This study shows that acetate metabolism in cumulus cells decreases with maternal age and correlates with oocyte maturity.

Subcellular metabolic pathway kinetics are revealed by correcting for artifactual post harvest metabolism

The paper corrects for artifactual post-harvest metabolism in subcellular isotope tracing to reveal compartment-specific metabolic kinetics.

Suppression of p16 increases nucleotide synthesis via mTORC1

This study shows that p16 suppression increases nucleotide synthesis through mTORC1 activation, promoting tumorigenesis by bypassing senescence.

Targeting IDH1 as a pro-senescent therapy in high-grade serous ovarian cancer

This study identifies IDH1 as a therapeutic target in HGSC by showing its role in promoting metabolic adaptation and epigenetic repression, leading to senescence when inhibited.

Simultaneous isotope dilution quantification and metabolic tracing of deoxyribonucleotides by liquid chromatography high resolution mass spectrometry

This paper develops a method to simultaneously quantify and trace dNTP metabolites using isotope dilution and high-resolution mass spectrometry, showing improved accuracy and metabolic pathway insights.

Artefactual formation of pyruvate from in-source conversion of lactate

The paper identifies an artefactual pyruvate formation from lactate during LC-MS analysis, highlighting the need for improved resolution or quantification in metabolomics studies.

Bioorthogonal pro-metabolites for profiling short chain fatty acylation

This paper introduces bioorthogonal pro-metabolites to profile short chain fatty acylation in cells, identifying novel protein targets and characterizing their metabolic and enzymatic effects.