S. Kannan

Modulation of Ideonella sakaiensis PETase active site flexibility and activity on morphologically distinct substrates by surface charge engineering

The paper investigates how surface charge mutations affect PETase activity on different PET substrates and identifies an engineered variant with improved degradation efficiency.

Rarγ-Foxa1 signaling promotes luminal identity in prostate progenitors and is disrupted in prostate cancer

This study shows RA signaling promotes luminal identity in prostate progenitors through Foxa1, and identifies a loss-of-function mutation in Foxa1 linked to prostate cancer dedifferentiation.

Single B cell transcriptomics identifies multiple isotypes of broadly neutralizing antibodies against flaviviruses

The paper uses single-cell transcriptomics to identify new broadly neutralizing antibodies against flaviviruses, including an IgA1 isotype with unique neutralizing properties.

ASPSCR1-TFE3 reprograms transcription by organizing enhancer loops around hexameric VCP/p97

This study identifies VCP/p97 as an obligate co-factor of ASPSCR1-TFE3, showing it organizes enhancer loops to drive oncogenic transcription in ASPS and RCC.

Expanding the DNA editing toolbox: novel lambda integrase variants targeting microalgal and human genome sequences.

This paper describes the directed evolution of lambda integrase variants that target microalgal and human genome sequences, showing enhanced specificity for non-natural sites.

A Gene Therapy for Hereditary Nonpolyposis Colorectal Cancer using CRISPR-Cas9 Nickase

This paper describes a CRISPR-Cas9 nickase-based gene therapy protocol to restore DNA mismatch repair in HNPCC by targeting MLH1 and MSH2 mutations in organoids and mouse models.

Single-cell RNA sequencing identifies response of renal lymphatic endothelial cells to acute kidney injury

This study uses single-cell RNA sequencing to characterize renal lymphatic endothelial cells' response to acute kidney injury and identifies gene expression changes across different injury models.

Mapping RUNX2 transcriptional dynamics during multi-lineage differentiation of human mesenchymal stem cells

The paper studies RUNX2 protein dynamics during hMSC differentiation into chondro-, osteo-, and adipogenic lineages using TF-FRAP and cluster analysis.

Design-Rules for Stapled Alpha-Helical Peptides with On-Target In Vivo Activity: Application toMdm2/X dual antagonists

The paper develops design rules for stapled alpha-helical peptides to improve in vivo activity and reduce off-target effects, demonstrating significant improvements in potency and safety.

Elamipretide Improves ADP Sensitivity in Aged Mitochondria by Increasing Uptake through the Adenine Nucleotide Translocator (ANT)

This paper shows that Elamipretide improves ADP sensitivity in aged mitochondria by enhancing ADP uptake through the ANT, leading to improved muscle and heart function.

Stitched peptides as potential cell permeable inhibitors of oncogenic DAXX protein

The paper designs and tests stapled peptides that inhibit DAXX, a protein involved in cancer, showing they enter cells and disrupt its interactions with other proteins.

Multiplatform Modeling of Atrial Fibrillation Identifies Phospholamban as Central Regulator of Cardiac Rhythm

This paper develops a multi-model platform to study atrial fibrillation-associated genes, identifying phospholamban as a key regulator of cardiac rhythm through interactions with calcium handling proteins.

Discovery of cell active macrocyclic peptides with on-target inhibition of KRAS signaling

This paper identifies macrocyclic peptides that inhibit KRAS signaling by binding to its hydrophobic pocket, showing on-target activity and improved proteolytic stability.

Conserved Transcription Factors Control Chromatin Accessibility and Gene Expression to Maintain Cell Fate Stability and Restrict Reprogramming of Differentiated Cells

This study identifies four conserved transcription factors that oppose cell fate reprogramming by controlling chromatin accessibility and gene expression, and shows their potential as therapeutic targets for regenerative medicine.

Trajectory reconstruction identifies dysregulation of perinatal maturation programs in pluripotent stem cell-derived cardiomyocytes

This study compares in vivo and in vitro cardiomyocyte maturation using single-cell RNA-seq, identifying dysregulated transcription factors in pluripotent stem cell-derived cardiomyocytes.

Src activates retrograde membrane traffic through phosphorylation of GBF1

This study identifies how Src kinase promotes retrograde membrane traffic by phosphorylating GBF1, facilitating its interaction with Arf1 and enabling GALNTs relocation from Golgi to ER.

Adhatoda Vasica ameliorates cellular hypoxia dependent mitochondrial dysfunction in acute and severe asthmatic mice.

This study shows Adhatoda Vasica extract reduces hypoxia-induced mitochondrial dysfunction and inflammation in asthma models, offering potential for treating severe steroid-resistant asthma.

Critical role for cold shock protein YB-1 in cytokinesis

This study identifies YB-1's role in cytokinesis by showing its necessity for microtubule organization and phosphorylation-dependent function.

Macrocyclization of an all-D linear peptide improves target affinity and imparts cellular activity: A novel stapled α-helical peptide modality

This paper combines macrocyclization and D-amino acid strategies to create an all-D helical peptide with improved target affinity and cellular activity.

The hunger games: sensing host arginine is essential for Leishmania parasite virulence

This study shows that Leishmania parasites use an arginine sensing mechanism to enhance virulence by up-regulating arginine transport during infection.

Vaccine-induced ICOS+CD38+ cTfh are sensitive biosensors of age-related changes in inflammatory pathways

The paper uses transcriptional profiling to show that vaccine-induced ICOS+CD38+ cTfh cells in elderly show inflammation-related gene signatures and age-associated biological changes.

Large particle fluorescence-activated cell sorting enables high quality single cell RNA-sequencing and functional analysis of adult cardiomyocytes

This study demonstrates that large-particle fluorescence-activated cell sorting (LP-FACS) improves the isolation of viable adult cardiomyocytes, enabling high-quality single-cell RNA sequencing and functional analysis.

De-risking drug discovery of intracellular targeting peptides: screening strategies to eliminate false-positive hits

This paper identifies false-positive peptide inhibitors of KRas using orthogonal assays and cellular screens, highlighting the need for rigorous validation in drug discovery.

Segmentation of Glomeruli Within Trichrome Images Using Deep Learning

This paper develops a deep learning framework to segment glomeruli in trichrome-stained kidney biopsy images, achieving high accuracy in distinguishing glomerular regions.