Advanced Fluorescence Microscopy Techniques

BeadBuddy: user-friendly, nanometer-scale registration of single-molecule imaging data

BeadBuddy corrects chromatic errors in single-molecule imaging data using fluorescent bead images, achieving sub-voxel resolution in DNA FISH and applicable across SMLM modalities.

A green lifetime biosensor for calcium that remains bright over its full dynamic range

The paper describes a new calcium biosensor, G-Ca-FLITS, that maintains high brightness in both calcium-bound and -unbound states, enabling more accurate and consistent measurements.

Absolute Membrane Potential Recording with ASAP-Type Genetically Encoded Voltage Indicators Using Fluorescence Lifetime Imaging

This paper evaluates genetically encoded voltage indicators using FLIM to measure absolute membrane potential, finding that rEstus-NI provides the highest sensitivity and accuracy.

Two-photon characterisation of long-Stokes-shift dye ATTO 490LS for single-laser multicolour imaging

The paper characterizes the two-photon properties of ATTO 490LS and demonstrates its use in multicolour two-photon imaging with a single laser.

Polarization MultiFocus Microscopy for volumetric super-resolution and orientation imaging of biofilaments

This paper introduces PolMFM, a method for simultaneous 3D localization and orientation imaging of single molecules, validated on actin filaments and chromatin in crickets.

Multiplexed imaging of G-proteins and ERK activity upon activation of CaSR

The paper develops a method to track G-protein and ERK activities in single cells using biosensors but finds challenges in obtaining robust FRET measurements.

Real-time simultaneous monitoring of multiple analytes in bacterial cultures

This paper introduces a method to monitor multiple bacterial metabolites in real-time using engineered periplasmic binding proteins in E. coli cultures.

Calibration-free molecular counting from a single DNA-PAINT intensity trace using cumulants

This paper introduces mCOAST, a calibration-free method for accurate molecular counting from DNA-PAINT intensity traces without requiring prior knowledge or extensive post-processing.

Parameter Optimization for Iterative MINFLUX Microscopy enabled Single Particle Tracking

The paper introduces a parameter optimization strategy for iterative MINFLUX microscopy to improve single particle tracking of fast lipid diffusion in membranes.

Isotropic, aberration-corrected light sheet microscopy for rapid high-resolution imaging of cleared tissue

This paper introduces a high-speed light sheet microscope with isotropic sub-micron resolution for cleared tissue imaging, achieving 850 nm resolution across 1 cm3 samples.

Two Novel Red-FRET ERK Biosensors in the 670-720nm Range.

This paper develops two red-FRET ERK biosensors, REKAR67 and REKAR76, which operate in the 670-720 nm range and are spectrally compatible with existing biosensors for single-cell ERK activity measurement.

GluTrooper: a novel reporter mouse line for whole-brain imaging of glutamate dynamics

GluTrooper is a mouse line for whole-brain glutamate imaging with long-lasting sensor expression and multimodal imaging capabilities.

Chamber Implant for Chronic Optical Recordings from the Cerebral Cortex of Marmosets

This paper describes a novel titanium imaging chamber for chronic optical recordings in marmosets, demonstrating long-term two-photon imaging quality and feasibility of voltage sensor imaging.

Miniaturized widefield microscope for high speed voltage imaging

The paper presents a miniaturized widefield microscope for high-speed voltage imaging in freely moving animals, achieving a peak-to-noise ratio >3 at 530 Hz.

Fast Hyperspectral and Super-Resolved Mapping of Lipid Membrane Polarity with Single-Molecule Sensitivity

This paper introduces SP-SMLM, a hyperspectral and super-resolution imaging method that maps lipid membrane polarity with high spatial and temporal resolution in live cells.

Pan-cortical cellular imaging in freely behaving mice using a miniaturized micro-camera array microscope (mini-MCAM)

The paper introduces a miniaturized micro-camera array microscope (mini-MCAM) for wide-field calcium imaging in freely behaving mice, enabling cortex-wide neural activity monitoring.

Confocal-Compatible Workflow for Sectioning, Staining, and Imaging Serial Vibratome Sections for 3D Anatomical Reconstruction of the Lymph Node

This paper presents a confocal-compatible workflow for 3D anatomical reconstruction of murine lymph nodes using vibratome sectioning and immunostaining.

Universal super-resolution for subcellular fluorescence imaging

This paper introduces a universal super-resolution framework that works across microscopes and subcellular structures without requiring optical modifications or prior sample knowledge, achieving high spatiotemporal resolution in live-cell imaging.

Leonardo: a toolset to correct sample-induced artifacts in light sheet microscopy images

Leonardo corrects sample-induced artifacts in light sheet microscopy images using two modules, validated on diverse biological samples.

Dual-view microscopy of single-molecule dipole orientations

This paper introduces dual-view microscopy to measure single-molecule dipole orientations with improved precision and three-dimensional accuracy.

A photoswitchable HaloTag for spatiotemporal control of fluorescence in living cells

This paper introduces a photoswitchable HaloTag (psHaloTag) that enables reversible fluorescence control in living cells using light.

STED-FCS in subdiffraction limit volumes reveals altered diffusion in live cell applications

This study demonstrates the application of STED-FCS in live cell assays to analyze molecular diffusion, showing its utility in studying autophagy, glycoprotein dynamics, and lipid mobility in disease contexts.

Tailoring the self-blinking of sulfonamide rhodamine for long-term protein-localizing super-resolution imaging

This paper develops a sulfonamide rhodamine with reduced self-blinking for extended super-resolution imaging of proteins in living cells.

Real-time, High-throughput Super-resolution Microscopy via Panoramic Integration

This paper introduces SPI, a super-resolution microscopy technique that enables real-time, high-throughput imaging with minimal post-processing.

StayRose: a photostable StayGold derivative red-shifted by genetic code expansion

The paper describes the creation of StayRose, a red-shifted variant of StayGold using genetic code expansion, which retains photostability and can be monomerised.

Nanoscale volumetric fluorescence imaging via photochemical sectioning

The paper develops photochemical sectioning to enable nanoscale volumetric imaging of whole-mount specimens, demonstrating its application in analyzing axon degeneration and myelination in mouse olfactory bulbs.

SiR-XActin: A fluorescent probe for imaging actin dynamics in live cells

The paper introduces SiR-XActin, a far-red fluorescent probe for imaging actin dynamics in live cells with minimal cytotoxicity and without genetic modifications.

Rapid adaptive optics enabling near noninvasive high-resolution brain imaging in awake behaving mice

This study develops MD-FSS for high-resolution brain imaging in awake mice, revealing differences in microglial states and vascular dynamics compared to anesthetized conditions.

FETCH enables fluorescent labeling of membrane proteins in vivo with spatiotemporal control in Drosophila

FETCH enables precise fluorescent labeling of membrane proteins in Drosophila with spatiotemporal control through covalent bond formation.

Mapping Mesoscale Cardiac Architecture with Axially Swept Dithered Light-Sheet Microscopy

The paper introduces a new microscopy technique to improve imaging of cardiac structures across multiple scales and stages.