Advanced Fluorescence Microscopy Techniques

Seeing in the Dark: Intelligent Fourier Light Field Imaging for Bioluminescence Microscopy

This paper develops a Fourier light field microscope with deep learning for fast, high-resolution bioluminescence imaging, demonstrating its use in 3D calcium imaging and cell dynamics analysis.

Calibration of FRET-based biosensors using multiplexed biosensor barcoding

This study introduces a calibration method for FRET biosensors using barcoded cells to normalize signals and enable reliable comparisons across experiments.

Parallel frequency-multiplexed aberration measurement for widefield fluorescence microscopy

This paper introduces PFAM and PFAM-SIFT methods for parallel aberration measurement in widefield fluorescence microscopy, enabling accurate correction of spatially varying aberrations in complex samples.

Ultra-low photodamage three-photon microscopy assisted by neural network for monitoring regenerative myogenesis

This paper develops a neural network to reduce photodamage in three-photon microscopy, enabling faster and safer imaging of muscle regeneration.

Direct single-molecule detection and super-resolution imaging with a low-cost portable smartphone-based microscope

This paper introduces a low-cost smartphone-based microscope capable of single-molecule detection and super-resolution imaging without signal amplification.

A nanobody-gold conjugate for the detection of GFP-tagged synaptic proteins by dual light-electron microscopy

The paper develops a nanobody-gold conjugate for dual light-electron microscopy to label GFP-tagged synaptic proteins with high specificity and nanoscale resolution.

Nanoscale spatial-omics via contrastive embedding of single-molecule localisation data

This paper introduces a contrastive learning framework for nanoscale spatial-omics, enabling analysis of single-molecule localisation data to study protein architecture and its changes under various conditions.

Single-aperture SLAM microscopy with amplitude-tailored vector beams

This paper introduces a single-aperture SLAM microscope using vector beams to achieve sub-diffraction-limited resolution in living tissue.

A versatile microfluidics platform for enhanced multi-target super-resolution microscopy

This paper introduces a microfluidics platform for multiplexed super-resolution microscopy, demonstrating its use in imaging cancer and cardiac cells with high reproducibility.

hpGRISZ: a high-performance fluorescent biosensor for in vivo imaging of synaptic zinc dynamics

The paper introduces hpGRISZ, a fluorescent biosensor for in vivo imaging of synaptic zinc dynamics, showing high responsiveness and suitability for detecting extracellular zinc release.

Multi-immersion Oblique Plane Microscope (miOPM): A reconfigurable platform for high-resolution Light-Sheet Fluorescence Microscopy.

The paper introduces miOPM, a reconfigurable LSFM platform that enables high-resolution imaging across multiple spatial scales and refractive indices.

Offsetting ROS-mediated arrest of endothelial fenestration dynamics permits long-term optical super-resolution- and AFM-imaging

The paper identifies ROS as a cause of fenestration arrest in LSECs during imaging and proposes a strategy to preserve dynamics for long-term super-resolution and AFM imaging.

Foundation model for efficient biological discovery in single-molecule data

The paper introduces META-SiM, a transformer-based model for analyzing single-molecule fluorescence microscopy data, and discovers a new intermediate state in pre-mRNA splicing.

Revealing the Ultrastructure of Live Candida albicans using Stimulated Emission Depletion Microscopy

This study uses STED microscopy to improve resolution of Candida albicans ultrastructure, showing better performance than confocal microscopy for tracking sub-cellular components over time.

SBIS, a new orange fluorescent vital probe for the 4D imaging of brown algal cells

The paper introduces SBIS, a fluorescent probe for 4D imaging of brown algal cells, enabling 3D visualization of cell morphology during embryogenesis in multiple species.

Nano-org, a functional resource for single-molecule localisation microscopy data

Nano-org provides a curated resource of single-molecule localisation microscopy data for studying protein nanoscale distributions and their relationships across cell types and conditions.

Single molecule spectrum dynamics imaging with 3D target-locking tracking

This paper introduces 3D-SpecDIM, a technique that captures both 3D positional and spectral dynamics of biomolecules, demonstrating its application in mitophagy and cellular blebbing.

Development of single-molecule enzyme activity assay for serine hydrolases using activity-based protein labeling probes

This paper develops a single-molecule enzyme activity assay for serine hydrolases using activity-based probes and demonstrates its application in detecting active granzyme B as a biomarker of liver damage.

Prediction of fast subcellular calcium signals based on label-free second harmonic generation microscopy measurements

The paper predicts calcium sparks using label-free SHG microscopy and pix2pix, showing agreement with ground truth data on frequency and location.

Determination of factors that allow cryogenic nanoscopy with high power illumination without devitrification

This study investigates laser-induced heating in cryogenic nanoscopy and identifies factors that prevent devitrification under high power illumination.

Robustness and fidelity of voltage imaging analysis pipelines

This study evaluates voltage imaging analysis pipelines, identifies their strengths and limitations, and proposes a decision-tree for selecting appropriate analysis strategies based on experimental conditions.

Observing biological spatio-angular structures and dynamics with statistical image reconstruction and polarized fluorescence microscopy

This paper introduces an algorithm to reconstruct 3D molecular orientation and density from polarized fluorescence microscopy data, demonstrating its application on biological structures and dynamics.

Time-resolved fluorescent proteins towards fluorescence microscopy in the temporal and spectral domains

This paper introduces time-resolved fluorescent proteins (TRFPs) that enable temporal-spectral resolved microscopy for multiplexed imaging and super-resolution in live cells.

Preservation of soluble enhanced green fluorescent protein (EGFP) within fixed NIH 3T3 fibroblasts

This study evaluates fixation protocols to preserve soluble EGFP fluorescence in NIH 3T3 cells, finding that MBS cross-linker in microtubule stabilizing buffer best preserves fluorescence intensity.

Interferometric Image Scanning Microscopy for label-free imaging at 120 nm resolution inside live cells

This paper introduces iISM, a label-free imaging technique with 120 nm resolution for live cell imaging, reducing photodamage and enabling long-term observation of subcellular structures.

Genetically Encoded Red Fluorescent Indicators for Imaging Intracellular and Extracellular Potassium Ions

This study develops two red genetically encoded potassium indicators (RGEPOs) for real-time imaging of intracellular and extracellular potassium dynamics in various biological systems.

Surface-Tethering Enhances Precision in Measuring Diffusion Within 3D Protein Condensates

This study shows surface tethering improves accuracy in measuring diffusion within 3D protein condensates by reducing motion artifacts while preserving their native properties.

CaMK2rep: A Highly Sensitive Genetically Encoded Biosensor for Monitoring CaMKII Activity in Mammalian Cells

The paper introduces CaMK2rep, a sensitive biosensor for detecting CaMKII activity, validated in HeLa cells and neurons, showing linear response and no cytotoxicity.

Two-Dimensional Nonlinear Structured Illumination Microscopy with rsEGFP2

The paper demonstrates 2D nonlinear structured illumination microscopy (NSIM) using rsEGFP2 to achieve sub-80 nm resolution for live-cell actin imaging.

Absolute measurement of fast and slow neuronal signals with fluorescence lifetime photometry at high temporal resolution

The paper develops FLIPR, a system for absolute fluorescence lifetime measurement in mice, revealing spatial and temporal variations in dopamine signaling.