Advanced Fluorescence Microscopy Techniques

PinkyCaMP a mScarlet-based calcium sensor with exceptional brightness, photostability, and multiplexing capabilities

The paper introduces PinkyCaMP, a new red fluorescent calcium sensor with improved brightness, photostability, and compatibility with optogenetics and neurotransmitter imaging.

Compressive axial-integrated planar scanning (CAPS) microscopy for high-speed volumetric imaging of cardiac dynamics

This paper introduces CAPS microscopy to enable high-speed volumetric imaging of cardiac dynamics in zebrafish larvae with improved spatiotemporal resolution and throughput.

Dual-Channel Event Microscopy for Ultrafast Biological Imaging

This paper introduces Dual-Channel Event Microscopy (DEM) for ultrafast, volumetric imaging of biological processes, demonstrating its ability to capture fast dynamics in zebrafish with high temporal resolution and spectral separation.

A stable cryogenic fluorescence microscope for correlative super-resolution light and electron microscopy

This paper introduces a modular cryogenic light microscope for single-molecule localization microscopy, improving stability and reducing ice contamination in cryo-CLEM.

Structure-guided, physics-aware reconstruction expands scan-limited multiphoton imaging

SPARC improves temporal and spatial resolution in scan-limited multiphoton imaging by integrating denoising and deep upsampling with structural and physical constraints.

Cryo-FIB lamellae enable ultra-high excitation laser intensities for cryogenic super-resolution fluorescence imaging

This paper demonstrates that cryo-FIB lamellae allow higher excitation laser intensities in SR-cryo-CLEM, improving localization precision and imaging speed.

Synthetic multicolor antigen-stabilizable nanobody platform for intersectional labelling and functional imaging.

The paper introduces a synthetic nanobody platform for multicolor labeling and functional imaging, enabling precise tracking of protein dynamics and cellular processes.

Quantitative mapping of nanoscale EGFR-Grb2 assemblies by DNA-PAINT

This study uses DNA-PAINT to quantitatively map nanoscale EGFR-Grb2 assemblies in cells after EGF stimulation, revealing changes in EGFR density and Grb2 accumulation.

High-Speed Multiplexed DNA-PAINT Imaging of Nuclear Organization Using an Expanded Sequence Repertoire

This paper introduces speed-optimized DNA-PAINT sequences for high-plex super-resolution imaging and applies them to analyze nuclear organization changes under transcription inhibition.

Anchored Brownian motion and Bayesian methods for the analysis of single particle tracking data

The paper introduces a Bayesian method based on anchored Brownian motion to estimate diffusion coefficients from single particle tracking data, showing improved performance over conventional methods.

Interferometric Ultra-High Resolution 3D Imaging through Brain Sections

This paper introduces 4Pi-BRAINSPOT, a method for ultra-high-resolution 3D imaging of molecular structures in brain tissue with sub-15-nm resolution.

SIMBA: an agentic AI platform for single-molecule multi-dimensional imaging

SIMBA is an AI platform that integrates single-molecule imaging analysis with deep learning, enabling automated and interactive processing of multi-dimensional fluorescence data.

Ratiometric Fluorescent Protein Biosensors Reveal Citrate Dynamics and Cellular Heterogeneity

This paper develops excitation ratiometric fluorescent protein biosensors for citrate, enabling quantitative analysis of citrate dynamics in different cellular compartments and populations.

Inferring norepinephrine dynamics from partial observations reveals the temporal structure of elevations during arousal

The paper develops methods to infer norepinephrine dynamics from partial observations, revealing temporal patterns during arousal.

HYPER-Net: Physics-Conditioned Self-Supervised Reconstruction for Fourier Light-Field Microscopy

HYPER-Net enables physics-conditioned self-supervised reconstruction for Fourier light-field microscopy, improving volumetric imaging across diverse biological contexts.

Simulating Multi-Colour Single-Molecule Localisation Microscopy Using an RGB Camera

This paper demonstrates that RGB cameras can effectively classify up to six fluorophores in SMLM with high precision, offering a cost-effective alternative to spectral imaging.

Adapting a two-photon scanning microscope for simultaneous single-photon imaging of an infrared dopamine sensor

The paper adapts a two-photon microscope to detect both visible and near-infrared fluorescence, enabling simultaneous dopamine and calcium imaging in the retina.

HAK-actin, U-ExM-compatible probe to image the actin cytoskeleton

The paper introduces HAK-actin, a probe for imaging actin cytoskeleton compatible with U-ExM, showing robust staining across human cells, microbial eukaryotes, and mouse retinal tissue.

Light-sheet excitation-encoded volumetric spectroscopy for fast multiplexed imaging and quantitative physicochemical mapping in cells

This paper introduces LS-ExSM, a method for fast multiplexed imaging and quantitative physicochemical mapping in cells, demonstrating six-color volumetric imaging and lipid-droplet polarity mapping.

On-lamella super-resolution cryo-CLEM for cryo-ET enabled by vacuum-free ultra-stable cryogenic fluorescence microscopy

This paper introduces VULCROM, a vacuum-free cryogenic fluorescence microscope that enables high-resolution cryo-SR-CLEM and detailed single-molecule studies in vitrified specimens.

An open, integrated platform for multiplexed bioluminescence microscopy

The paper introduces an open platform for multiplexed bioluminescence and fluorescence microscopy, including tools for experiment design, microscope upgrades, and data analysis.

Designer indicators for two-photon recording of subthreshold voltage dynamics

The paper develops two new genetically encoded voltage indicators (JEDI3sub and JEDI3hyp) for improved two-photon recording of subthreshold voltage dynamics in vivo.

Single-molecule imaging and tracking on clinical liquid biopsies reveals cancer biomarkers nanoscale organization and heterogeneity

This paper develops a method to analyze cancer biomarkers at the nanoscale using single-molecule imaging on clinical liquid biopsies, revealing patient-specific heterogeneity and enabling cancer cell classification.

Advanced Ellis Concept for a Fiber-Optic Fluorescent Microscope.

This paper introduces an advanced fiber-optic fluorescent microscope design that replaces traditional filter cubes with a vibrating optical fiber connected to a laser, enabling full spectrum fluorescence research.

Ultra-wide-field, deep, adaptive two-photon microscopy

This paper introduces a high-resolution two-photon microscopy system capable of imaging large brain regions with cellular resolution.

Characterization of Nanoparticles in Suspension by Simultaneous iNTA and Fluorescence Detection with Single-Molecule Sensitivity

This paper introduces iNTA-F, a method combining interferometric nanoparticle tracking analysis with fluorescence detection to characterize nanoparticles with single-molecule sensitivity.

Impact of Fluorophore and Epitope Position on Destabilized Reporter Performance in C. elegans

The paper investigates how fluorophore choice and epitope positioning affect the oscillatory behavior of destabilized reporter proteins in C. elegans.

A versatile, positive-going voltage indicator that enables accessible two-photon recordings in vivo

The paper introduces FORCE1s, a GEVI that enables robust two-photon voltage imaging in awake mice with high signal-to-noise and reliable spike detection.

Image-scanning light-sheet microscopy for high-speed volumetric imaging of complex biological dynamics

The paper introduces image-scanning light-sheet microscopy to enable high-speed volumetric imaging of complex biological dynamics with improved speed while maintaining resolution and efficiency.

Three photon microscopy of mouse brain structure and function at 2 mm depth and beyond

This paper demonstrates a three-photon microscope that enables high-resolution imaging of mouse brain structure and function at depths up to 2.5 mm and 2 mm respectively.