Advanced Fluorescence Microscopy Techniques

Parsing Contributions of Physical Phenomena to smFRET Statistical Inhomogeneity via Multiparameter Stochastic Simulations

The paper develops a simulation framework to analyze how different physical processes affect smFRET data by isolating and varying their contributions.

Quantitative comparison of fluorescent reporters by FCS excitation scan

The paper develops an FCS excitation scan assay to compare fluorescent reporters' brightness and photobleaching in tissue culture and zebrafish embryos, finding mNeonGreen outperforms mEGFP.

Side-by-side systematic characterization of novel FPs in budding yeast

The paper systematically compares novel fluorescent proteins in budding yeast to evaluate their characteristics for research applications.

Unveiling the Molecular Architecture of T Cells and Immune Synapses with Cryo-Expansion Microscopy

This study uses cryo-expansion microscopy to visualize the nanoscale architecture of immune synapses in T cells and their interactions with tumor cells, revealing structural details of signaling molecules and lytic granules.

Fluorescence-based mapping of condensate dielectric permittivity uncovers hydrophobicity-driven membrane interactions

This study maps condensate dielectric permittivity using fluorescence and reveals that membrane interactions depend on permittivity contrast rather than absolute permittivity.

Maximizing photon utilization in spectroscopic single-molecule localization microscopy using symmetrically dispersed dual-wedge prisms

This paper introduces a symmetrically dispersed dual-wedge prism design for spectroscopic single-molecule localization microscopy, improving lateral and spectral precision.

Cost-function Optimized Maximal Overlap Drift Estimation for Single Molecule Localization Microscopy

This paper introduces COMET, a drift correction method for SMLM that improves resolution and temporal precision, and demonstrates its effectiveness across multiple SMLM techniques.

3D Imaging with Superior Resolution Using Atacama Clear

This paper introduces Atacama Clear (ATC), a 3D imaging technology that improves resolution and signal detection in cleared tissues, enabling better analysis of complex biological samples.

A dual-color miniature endoscope for calcium imaging in behaving mice

This paper introduces a dual-color miniature endoscope for simultaneous calcium imaging of two neuronal populations in behaving mice, revealing direction-dependent activity patterns in striatal neurons.

Kilohertz-rate two-photon voltage imaging of population dynamics in vivo

This paper introduces HS2PM, a two-photon microscope that enables kilohertz-rate imaging of neuronal populations in vivo, revealing voltage dynamics and their role in sensory adaptation and population coding.

Hydrogel Fiber Endomicroscopy

This paper introduces HYFEN, a hydrogel-based endomicroscopy platform that enables flexible, biocompatible, and subcellular-scale fluorescence imaging with improved resolution and performance compared to conventional silica-based fibers.

Intensity Modulation of Trichromatic Split Fluorescent Proteins for Live Cell Mapping

The paper introduces Caterpie, a live cell labeling system using split fluorescent proteins to enable high-fidelity identification of multiple cell populations with 97% accuracy.

Fluorogenic speed-optimized DNA-PAINT probes enable super-resolution imaging of whole cells

This paper introduces fluorogenic DNA-PAINT probes that improve super-resolution imaging speed and signal-to-background ratio in cells.

A high-affinity split-HaloTag for live-cell protein labeling

This paper introduces a high-affinity split-HaloTag system for live-cell protein labeling, enabling efficient and versatile imaging with compatibility for advanced microscopy techniques.

Back-illumination Phase Imaging Enables Nanoscale Drift Stabilization in Non-transparent Biological Tissues

This paper introduces a method for nanoscale drift stabilization in thick non-transparent tissues using back-illumination phase imaging and cross-correlation analysis, enabling high-quality imaging of extracellular structures.

Diffractive scanning live volumetric two-photon microscopy within the contracting mouse intestine

This study uses 4D-SLIDE microscopy to image the enteric nervous system in intact mouse intestines during contractions, resolving micrometer-scale movements without photodamage.

ATPLyzer An advanced ratiometric multi-colour biosensor for long-term monitoring of ATP dynamics

The paper introduces ATPLyzer, a ratiometric biosensor for monitoring ATP dynamics, showing its utility in E. coli and demonstrating growth-phase and carbon-supply-dependent ATP changes.

Automated Cyclic Super-Resolution Microscopy for Nanoscale Protein Mapping

The paper introduces CycSTORM, an automated cyclic (d)STORM platform for multiplexed nanoscale protein mapping in single cells, achieving sub-5 nm precision and reducing signal variability across imaging cycles.

Dark state-mediated photobleaching in mCherry-basedred fluorescent proteins

This study investigates dark state-mediated photobleaching in mCherry-based RFPs, showing photodestructive dark states and providing insights for engineering brighter and more photostable probes.

FLIM-FRET as a Molecular Filter for Membrane-Induced Aggregation

This paper introduces a FLIM-FRET method to quantify membrane-proximal alpha-synuclein aggregation in neurons by estimating per-cell lifetimes and mixture fractions using a hierarchical EM algorithm.

MiniFAST: A sensitive and fast miniaturized microscope for in vivo neural recording

The paper introduces MiniFAST, a miniaturized microscope that improves speed, sensitivity, and resolution for in vivo neural recording using genetically encoded indicators.

Wash-Free Multi-Target Super-Resolution Microscopy with Photocaged DNA Labels

This paper introduces a wash-free method for multi-target super-resolution microscopy using photocaged DNA labels, demonstrating improved imaging accuracy without sample manipulation.

A genetically encoded fluorescent sensor for monitoring spatiotemporal prostaglandin E2 dynamics in vivo

This paper develops a genetically encoded fluorescent sensor, GRABPGE2-1.0, to monitor spatiotemporal PGE2 dynamics in vivo with high resolution.

Kinetic design of reversible probe exchange enables continuous single-molecule tracking beyond the photobleaching limit

This paper introduces a method to enable continuous single-molecule tracking beyond photobleaching limits by designing reversible probe exchange kinetics.

Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo

This paper develops a minimally invasive optical clearing medium for live mammalian tissue imaging, enabling structural and functional imaging with minimal impact on cellular properties.

Single-molecule FRET with a minimalistic 3D-printed setup and dyes in the blue-green spectral region

This paper introduces a minimalistic 3D-printed setup for single-molecule FRET using blue-green dyes and photostabilizers, demonstrating its effectiveness for conformational studies.

A universal protein ladder for standardisation of diverse FRET assays

The paper introduces a universal protein ladder to standardize FRET assays across different platforms and experimental conditions.

3D-MINSTED nanoscopy and protein tracking in densely labelled living cells

This paper introduces a 3D MINSTED nanoscopy method for tracking single proteins in live cells with sub-nanometer precision, demonstrating its application to kinesin-1 movement along microtubules.

Quantitative spectral Linear Unmixing and Ratiometric FRET for live-cell imaging of protein interactions

The paper introduces a live-cell imaging method combining spectral linear unmixing and ratiometric FRET to quantify protein interactions and signaling dynamics with high spatial and temporal resolution.

FLEXTAG: A Small and Self-renewable Protein Labeling System for Anti-fading Multi-color Super-resolution Imaging

FLEXTAG improves super-resolution imaging by enabling long-term, multi-color, low-bleaching labeling of subcellular structures in live and fixed cells.