Glycosylation and Glycoproteins Research

An integrated mass spectrometry strategy for quantifying the proteoform diversity of the extensively modified O-glycoprotein Osteopontin

This study develops an integrated mass spectrometry strategy to quantify the proteoform diversity of O-glycoprotein osteopontin, revealing systematic underestimation of glycan sialylation by bottom-up methods.

Cell Surface Sialoglycan Engineering Through Exogenous mRNA

This paper develops an mRNA-based platform to transiently control sialoglycan biosynthesis on human cells by delivering sialyltransferase enzymes, showing increased surface presentation of specific sialoglycans with minimal off-target effects.

Non-Carbohydrate Inhibitors of Sialic Acid-binding Immunomodulatory-type Lectin-7 (Siglec-7) Discovered from Genetically Encoded Bicyclic Peptide Libraries

This paper discovers non-carbohydrate inhibitors of Siglec-7:GD3 interactions using BiGEL libraries and validates their potency through SPR, ELISA, and NMR.

HUMAN-LIKE SIALOME REMODELING LINKS HYPERGLYCEMIA TO COLORECTAL CANCER PROGRESSION

This study shows that a human-like sialome increases susceptibility to metabolic stress in colorectal cancer, using a mouse model with Cmah deficiency and Apc mutation.

Co-translational O-glycosylation driven by GALNTs spatial reprogramming fosters pancreatic cancer growth

The paper shows that GALA, a process relocating GALNTs to the ER, enables co-translational O-glycosylation in pancreatic cancer, expanding the O-glycoproteome and promoting tumor growth.

Metabolomics of in vivo inflammation identifies soluble sialic acid as a conserved myeloid-cell metabolite

The paper identifies soluble sialic acid as a conserved biomarker of inflammation through metabolomics in mouse tissues and cultured macrophages.

Molecular Determinants of Sequence-Dependent N-glycosylation by the Human Oligosaccharyltransferase

The paper defines the molecular basis of sequence-dependent N-glycosylation by human OST using simulations and experiments, confirming rules at proteome scale.

Micro- and macro- heterogeneity of N-glycoproteome remodeling in endoplasmic reticulum stress

The paper develops an mPBA enrichment workflow to analyze N-glycoproteome remodeling under ER stress, revealing dynamic changes in glycosylation sites and glycoforms.

S-Palmitoylation stabilizes OGT and the OGT-PPP1CC complex

This study identifies S-palmitoylation of OGT and shows it stabilizes OGT by preventing CMA degradation and enhances its interaction with PPP1CC and YAP.

Rewiring c-Myc Transcriptional Activity with an O-GlcNAcylation Targeting Chimera (OGTAC)

This paper introduces OGTAC, a molecule that enhances c-Myc O-GlcNAcylation, rewires its transcriptional activity, and inhibits cancer cell proliferation.

A Transient Increase in Cardiomyocyte Protein O-GlcNAcylation Enhances Susceptibility to Pressure Overload-Induced Cardiac Remodeling

This study shows that transiently increased cardiomyocyte O-GlcNAcylation worsens cardiac remodeling after pressure overload, supporting the concept of O-GlcNAc memory as part of metabolic memory.

GlyComboCLI enables command line-based FAIR workflows for glycan composition assignment in mass spectrometry data

GlyComboCLI assigns glycan compositions to mass spectrometry data through a command-line interface, supporting diverse parameters and integration into FAIR workflows.

Cancer Cell Lewis X Plays a Minor Role in NK Cell Immune Evasion

This study investigates the role of Lewis X (LeX) glycans in cancer cell evasion of NK cell-mediated destruction, finding that LeX has a limited impact compared to sialylated glycans.

Defining glycoproteoform landscapes through an integrated glycoproteomics approach enabled by high-resolving power proton transfer charge reduction tandem mass spectrometry

This paper introduces a high-resolving power DIA-PTCR method for glycoproteoform analysis, enabling isotopically resolved mass measurements and integration with other glycoproteomics approaches.

Proximity-induced protein deglycosylation by endogenous O-GlcNAcase

This paper introduces DOGTACs, a method to selectively remove O-GlcNAc from target proteins using endogenous OGA in living cells, showing concentration- and time-dependent de-O-GlcNAcylation without affecting global O-GlcNAc levels.

Enrichment-free glycoproteomics harnessing real-time mass defect-driven glycopeptide classification reveals sex differences in murine fucosylation

The paper develops an enrichment-free glycoproteomics method using mass defect-driven glycopeptide classification to identify sex differences in murine plasma fucosylation.

ST6GAL1-mediated sialyl linkage switching in tumor-associated macrophages drives cancer-promoting nanotubes carrying α2,6-sialylation in anti-inflammatory cells

This study identifies ST6GAL1-mediated sialyl linkage switching in TAMs that drives cancer-promoting nanotubes with α2,6-sialylation, affecting immune interactions and CRC progression.

N-glycome analysis of dried blood spots from different blood preparations and its potential for pre-diabetes and diabetes distinction

This study analyzes N-glycans in dried blood spots (DBS) from different blood preparations to distinguish pre-diabetes and diabetes, showing trends in fucosylation, bisection, galactosylation, and sialylation.

Different spatial profiles of aberrant N-glycans in pediatric and adult MOGHE brain tissue

The paper uses spatial glycomics to show that N-glycan abnormalities in MOGHE are linked to hypomyelination and are more pronounced in pediatric cases.

Extravillous trophoblast model shows generation of bioequivalent N-glycans can maintain immunological protection against natural killer cell cytotoxicity

The paper shows that specific N-glycan profiles in extravillous trophoblasts (EVTs) are critical for maintaining immunological protection against natural killer (NK) cell cytotoxicity.

Cell-surface N-linked glycans manipulation of K562 cells for augmented susceptibility to natural killer cell killing

This study shows that altering N-linked glycan terminal sialylation on K562 cells increases their susceptibility to NK-92 cell-mediated cytolysis.

Xylose phosphatase activity of dystroglycan self-regulates its receptor function

The paper identifies a glycan phosphatase function of dystroglycan that regulates matriglycan extension by removing phosphate from xylose.

Integrating GlycoSHIELD Modeling and DNA-PAINT SMLM to Map the Glycosylation-Dependent Distri-bution of the Na,K-ATPase

This study uses GlycoSHIELD modeling and DNA-PAINT SMLM to show that N-glycosylation promotes Na,K-ATPase clustering by forming a steric shield, supporting a galectin-lattice organization mechanism.

alpha2,3-sialylation on human naive T cells restrains bispecific engager-mediated anti-tumor immunity

Desialylation enhances bispecific T cell engager responses by relieving a sialoglycan-dependent restraint in human T cells.

GPMAW Glyco-Search: An Integrated Workflow for Identification and Validation of Intact Sialylated N-Glycopeptides

This paper introduces GPMAW glyco-search, a workflow for high-confidence identification of intact sialylated N-glycopeptides using TiO2 enrichment, dual LC-MS/MS, and interactive spectrum validation, achieving over 2800 identifications in human plasma.

T50 O-GlcNAc PKM2 promotes aerobic glycolysis and PDAC progression via ARNT-CDC27-AKT pathway

This study identifies an O-GlcNAc modification on PKM2 that promotes PDAC progression through the ARNT-CDC27-AKT pathway by enhancing aerobic glycolysis.

Sialyl-Tn-positive tumour-derived extracellular vesicles impair dendritic cell function via horizontal transfer of glycans

This study shows that STn-positive tumor-derived extracellular vesicles impair dendritic cell function by transferring glycans, leading to immunosuppression.

Salmonella SiiE-mediated apical invasion into colonocytes depends on MUC1 α2,3-linked sialic acids

This study shows Salmonella uses 2,3-sialylated MUC1 on colonocytes for apical invasion, identified through glycan analysis and ex vivo models.

Biological recognition of mirror-image glycans

The paper evaluates how glycan-binding proteins and immune cells recognize mirror-image glycans, finding widespread binding and donor-specific recognition of L-glycans.

Dynamic organizational strategies of multidomain glycosyltransferases revealed by high-speed AFM and solution biophysics

The paper uses high-speed AFM and biophysical methods to study dynamic conformational changes in multidomain glycosyltransferases, revealing diverse organizational strategies.