Glycosylation and Glycoproteins Research

How the terminal glucoside of the N-glycan donor affects the catalytic efficiency of the eukaryotic oligosaccharyltransferase

This study investigates how the terminal Glc-(1-2)- in the LLO donor affects OST catalytic efficiency through molecular dynamics simulations of yeast OST with and without this motif.

Development of a Matrix-Matched Calibration Curve for Multi-Site Quantification of Neu5Gc-Bearing N-Glycans

This paper develops a matrix-matched calibration curve for quantifying Neu5Gc-bearing N-glycans across multiple sites, showing LC-MS outperforms DMB-LC-FLR in dynamic range and sensitivity.

Spatial Glyco-Codes Define Human Liver Pathology and Progression

This study introduces spatial-GPT, a platform for spatial glycan, protein, and transcriptome profiling in human liver tissues, revealing glyco-codes that define disease states and progression.

Rheumatoid Arthritis-associated IgG N-glycan agalactosylation diminishes neutrophilic inflammation by reducing FcgammaR binding and downstream signaling

This study shows that agalactosylated IgG N-glycans in RA reduce neutrophilic inflammation by decreasing FcγR binding and downstream signaling, suggesting they may act as a compensatory anti-inflammatory mechanism.

Identification of novel cellular intermediates unveils unique enzymes for flagellar glycan biosynthesis in Clostridioides difficile

This study identifies two novel biosynthetic intermediates and characterizes their enzymes in the unique glycan modification pathway of C. difficile flagellin.

Contextualised real-time mass spectrometry improves glycosylation detection and characterisation

This paper introduces GlycoRTMS, a real-time mass spectrometry method that improves glycosylation detection by using glycan composition context to guide fragmentation and prioritization.

Discovery of cell-active small molecule inhibitors of UDP-galactose 4'-epimerase

This paper identifies disulfiram as a cell-active inhibitor of human GALE through high-throughput screening, showing its effects on glycosylation and mucin biosynthesis.

TUSC3 serves as a rate-limiting gatekeeper of a glycan-mediated ER Triage Checkpoint for BMP4/Dpp

The paper identifies TUSC3 as a gatekeeper regulating glycan trimming of BMP4/Dpp to control ERQC entry and BMP signaling.

Kaposi Sarcoma Herpes Virus Reprograms Mesenchymal Cell Glycosylation to control platelet-derived growth factor receptor A signaling

This paper identifies how KSHV alters mesenchymal cell glycosylation to enhance PDGFRA signaling, contributing to Kaposi sarcoma pathogenesis.

Iterative Bump-and-hole engineering creates a bioorthogonal reporter for N-acetylglucosaminyltransferase I

The paper develops a bioorthogonal reporter for MGAT1 by iteratively engineering a UDP-GlcNAc analog with a chemical tag, enabling specific substrate incorporation in mammalian cells.

B-cell SIGLEC-5 engages T-cell components of the elastin receptor complex (ERC) to suppress inflammatory T-cell cytokines

The paper identifies SIGLEC-5 as an inhibitory receptor on B-cells that suppresses T-cell inflammatory cytokines by engaging the elastin receptor complex.

GlycoDiveR: a modular R framework to analyze and visualize highly dimensional glycoproteomics data

GlycoDiveR provides a modular R framework for analyzing and visualizing high-dimensional glycoproteomics data, improving accessibility and flexibility in glycosylation analysis.

GALE-dependent glycoproteome remodelling is a determinant of oncogenic RAS transformation

This study identifies GALE as a key regulator of glycoproteome remodelling in RAS-driven cancer, showing its role in sialylation and tumorigenicity.

Pareto-optimal synthesis of multiple glycans in Golgi compartments

The paper models glycan synthesis in the Golgi apparatus to identify Pareto-optimal enzyme distributions that balance glycan yield and residence time trade-offs.

A Glycosylation-Dependent Checkpoint Restrains Intestinal Intra-Epithelial Lymphocyte Activation

This study identifies GCNT2 as a glycosylation-dependent checkpoint that restrains intestinal intra-epithelial lymphocyte (IEL) activation by modifying CD45 and dampening TCR signaling.

Characterizing the Effects of Protein Glycosylation Perturbation on Phosphorylation Signaling

This study shows that altering glycosylation in human cells changes phosphorylation signaling networks, with engineered cells showing reduced phosphorylation responses and rewired signaling pathways.

Structural Analysis of Prostate Cancer N-Glycans Using Graph-Based Structural Metrics

This study develops two graph-based indices to quantify N-glycan structural complexity in prostate cancer, showing they can distinguish disease-related glycans from mass-to-charge ratio and abundance.

An Altered Glycome Shapes IgA B-Cell Responses and Gut Immunity During Intestinal Inflammation

This study shows that reduced (2,6)-sialylation in SIgA and B cells during UC impairs their function and exacerbates intestinal inflammation.

Absence of EOGT Precludes Defective Development in Fringe-null Mouse Intestine

The paper shows that absence of EOGT prevents developmental defects in Fringe-null mouse intestine, highlighting EOGT's role in intestinal development.

The Ligand Preference of LRP1 is Regulated by O-glycans

The paper shows that O-glycans in LRP1's ligand-binding domains differentially modulate uptake of tau and amyloid beta, but not RAP or ApoE.

Substrate recognition and cleavage by mucin degrading O-glycopeptidases from the gut microbe Bacteroides caccae.

This study identifies and characterizes O-glycopeptidases from Bacteroides caccae, revealing their substrate selectivity and diversity in mucin degradation.

GlycoForge generates realistic glycomics data under known ground truth for rigorous method benchmarking

GlycoForge generates synthetic glycomics data with known ground truth to benchmark methods, supporting motif-level effects, batch effects, and missing data simulation.

An IgG-Optimized Enzyme-Linked Lectin Assay (ELLA) for Quantitative Analysis of Immunoglobulin Glycosylation

The paper develops an optimized ELLA assay for quantifying IgG glycosylation, improving reproducibility and cross-study comparability through standardized protocols and a quantitative surrogate standard.

Comprehensive profiling reveals Sialyl-Tn upregulation and prognostic value in prostate cancer

This study shows sTn is upregulated in prostate cancer and correlates with poor patient survival, suggesting it as a prognostic biomarker and therapeutic target.

Sialidase-Mediated Desialylation Regulating EGFR Phosphorylation and Signal Flux

This study shows that desialylation reduces EGFR phosphorylation and signaling by affecting specific glycoforms and phosphosites, highlighting glycan remodeling as a therapeutic strategy for RTK-driven cancers.

Genetic rescue of pathogenic O-GlcNAc dyshomeostasis associated with microcephaly and motor deficits

This study investigates OGA inhibition as a potential therapy for OGT-CDG by creating a mouse model and showing partial restoration of O-GlcNAc homeostasis.

Biosynthetic glycan modeling reveals metabolic shifts in disease

The paper introduces a structure-aware algorithm for glycan biosynthetic network construction and demonstrates its application in analyzing metabolic shifts in cancer.

Arthritis-Associated Inflammation Remodels Colonic O-Glycosylation

This study shows arthritis-associated inflammation reduces colonic mucin fucosylation, linking intestinal inflammation to fibroblast-driven microenvironment changes.

Structure and function of human NXPE1, a sialic acid O-acetyltransferase

This study determines the cryo-EM structure of human NXPE1, identifies it as a sialic acid O-acetyltransferase, and shows its role in intestinal barrier function relevant to IBD.

Lysosomal Aspartylglucosaminidase Couples Glycoprotein Catabolism to Cell-Surface GlycoRNA Production

The paper identifies lysosomal aspartylglucosaminidase (AGA) as a rate-limiting regulator linking glycoprotein catabolism to glycoRNA formation.