Mycobacterium research and diagnosis

Ecological Drivers of Nontuberculous Mycobacteria in Aquatic Systems: Biodiversity, Niche Competition, and Pathogen Emergence

The paper investigates ecological factors driving nontuberculous mycobacteria in aquatic systems, finding habitat type, abiotic factors, and microbial interactions influence their distribution and disease transmission.

Lower Airway Dysbiosis in NTM+ Bronchiectasis is Associated with NET-Predominant Severe Phenotypes

This study links lower airway microbiota and neutrophilic inflammation in NTM+ bronchiectasis to severe disease phenotypes through network analysis and preclinical models.

A highly conserved two-gene operon is crucial for lipoarabinomannan localization, pathogenesis, and cell envelope function in Mycobacterium abscessus

This study identifies the lprg-mfs operon as essential for virulence, lipid distribution, and cell envelope integrity in Mycobacterium abscessus.

A mobile ESX type VII secretion system enhances intracellular persistence in globally distributed Mycobacterium abscessus

This study identifies a novel plasmid-borne ESX type VII secretion system in M. abscessus that enhances intracellular survival in macrophages.

Species Identification and Antimicrobial Susceptibility of Mycobacterial Isolates from Dogs and Cats

This study identifies NTM species and their antimicrobial resistance in cats and dogs, finding rapid growers dominate and resistance varies by species.

Concentration-dependent suppression of macrolide resistance in Mycobacterium avium by combination therapy: an improved in vitro time-kill assay and PK/PD modelling study

This study quantifies how combination therapy with concomitant drugs suppresses macrolide resistance in M. avium using improved time-kill assays and PK/PD modeling.

Efficacies of sequenced monotherapies of Mycobacterium avium lung infection in mouse

This study evaluates sequential monotherapy for M. avium lung infection in mice, finding it as effective as multidrug therapy without promoting resistance.

CRISPRi-mediated validation of candidate Myocobacterium abscessus drug targets during host infection

This study uses CRISPRi to validate four candidate drug targets in Mycobacterium abscessus during infection, showing varying in vivo efficacy depending on host immune status.

A cross-environment comparison of nontuberculous mycobacterial diversity

This study compares nontuberculous mycobacterial diversity across surface waters, soil, and household plumbing biofilms, finding that plumbing biofilms have the highest abundance and occurrence of clinically relevant species.

Mycobacterial cell division arrest and smooth-to-rough envelope transition using CRISPRi-mediated genetic repression systems

This paper describes a CRISPRi-based protocol to assess gene silencing and envelope transitions in Mycobacterium smegmatis as a model for NTM pathogenesis.

Primary tuberculous mycobacterial granulomas provide a niche for superinfecting Mycobacterium abscessus

The paper shows that Mycobacterium abscessus grows preferentially in granulomas formed by prior Mycobacterium marinum infection in zebrafish, exploiting a nutrient-rich niche.

Distribution, Prevalence of Non-Tuberculous Mycobacteria in Hainan Island and Antibiotic Resistance of Mycobacterium abscessus

This study maps NTM distribution and antibiotic resistance in Hainan Island, identifying key species and resistance patterns to inform regional surveillance and treatment.

Defining new Buruli ulcer endemic areas in urban southeastern Australia using bacterial genomics-informed possum excreta surveys

Tigecycline pharmacodynamics in the hollow fiber system of Mycobacterium avium-complex lung disease, and the utility of MICs and time-kill studies in drug development

The paper evaluates tigecycline pharmacodynamics in MAC lung disease using time-kill studies and hollow fiber systems, finding EC50 values and suggesting inhaled tigecycline as a potential treatment.

Enhanced Activity of Apramycin and Apramycin-Based Combinations Against Mycobacteroides abscessus

This study compares the in vitro activity of apramycin and other aminoglycosides against Mycobacteroides abscessus and related species, finding apramycin more potent than amikacin.

A multiplexed, target-based phenotypic screening platform using CRISPR interference in Mycobacterium abscessus

This paper introduces a CRISPRi-based PROSPECT platform for drug discovery in M. abscessus, identifying new compound targets and revealing unexpected susceptibility to isoniazid.

A bioluminescence-based chemical screen identifies a bactericidal naphthalene scaffold targeting MmpL3 in Mycobacterium abscessus

Integrated analysis of Tn-Seq and pangenome reveals core and lineage-specific essential genes in Mycobacterium avium subsp. hominissuis.

The paper integrates Tn-Seq and pan-genome analysis to compare essential genes across MAH strains, finding core and lineage-specific essential genes.

Plasmid-mediated macrolide resistance among rapidly growing mycobacteria in Japan

This study identifies a low prevalence of erm(55)-carrying plasmids in Japanese RGM isolates, conferring inducible macrolide resistance.

GplR1, an unusual TetR-like transcription factor in Mycobacterium abscessus, controls the production of cell wall glycopeptidolipids, colony morphology, and virulence

This paper identifies GplR1, a transcription factor in Mycobacterium abscessus, as a regulator of glycopeptidolipid production, colony morphology, and virulence.

From colonisation to chronicity: adaptation of Mycobacterium abscessus in the cystic fibrosis lung environment

This study examines how Mycobacterium abscessus adapts during chronic infection in the CF lung, showing increased host cell attachment and intracellular survival in late isolates.

REBASTINIB, OLVEREMBATINIB, AND NAS-181 INHIBIT MYCOBACTERIUM ABSCESSUS BY DISRUPTING MYCOTHIOL HOMEOSTASIS

This study identifies rebastinib, olverembatinib, and NAS-181 as potential drugs that inhibit Mycobacterium abscessus by disrupting mycothiol homeostasis, showing efficacy in vitro and in vivo.

A refined low-dose murine model of Mycobacterium ulcerans infection to assess integrated immune networks in Buruli ulcer pathogenesis

This study develops a low-dose murine model of Mycobacterium ulcerans infection to analyze immune responses and identifies key cell types involved in disease progression.

Combinations comprising dual β-lactams and a β-lactamase inhibitor achieve optimal synergistic inhibition of Mycobacterium abscessus growth

This study shows that combinations of dual β-lactams and β-lactamase inhibitors synergistically inhibit Mycobacterium abscessus growth, challenging the traditional view of their mechanisms.

An unusual genetic switch controls Mycobacterium avium pathogenesis, antibiotic resistance and colony morphology

This study identifies a genetic switch involving IS1245 transposition and Erp/MarP that controls colony morphology, virulence, and antibiotic resistance in Mycobacterium avium.

An alveolus lung-on-a-chip model of Mycobacterium fortuitum lung infection

This study develops a humanized lung-on-a-chip model to study early immune responses to Mycobacterium fortuitum infection, revealing cytokine and protease inhibitor upregulation.

Systematic and quantitative analyses of pre-clinical Mycobacterium avium Lung Disease Tools for Drug Development and Transition from Animal Models to New Approach Methodologies

The paper benchmarks preclinical tools for Mycobacterium avium lung disease drug development, finding significant variability in PK/PD target attainment and recommending HFS-MAC for antibiotic ranking and translational studies.

Immunity against Mycobacterium avium induced by DAR-901 and BCG

This study compares the efficacy of DAR-901 and BCG vaccines in inducing immunity against Mycobacterium avium in mice, showing similar cross-reactive immunity and protection against aerosolized MAC infection.

Divergent MIC of Clarithromycin and Azithromycin Against Clinical Isolates of Mycobacterium abscessus Complex

This study compares the in vitro activity of clarithromycin and azithromycin against M. abscessus complex isolates, finding significant resistance differences and discordance in susceptibility profiles.

Divergent Minimum Inhibitory Concentrations of Clarithromycin and Azithromycin Against Clinical Isolates of Mycobacterium abscessus Complex

This study compares the in vitro activity of clarithromycin and azithromycin against M. abscessus complex isolates, finding significant resistance differences and discordance in susceptibility profiles.