ATP Synthase and ATPases Research

Pharmacological inhibition of V-ATPase targets mode-switching but not the proton transport cycle

This study shows that V-ATPase inhibitors block mode-switching between active and inactive states without affecting proton transport rates.

In situ structure determination of Respiratory Supercomplexes and ATP synthase oligomers in mammalian mitochondrial inner membrane.

The paper determines the in situ structures of respiratory supercomplexes and ATP synthase oligomers in bovine heart mitochondria using cryoEM, revealing new structural arrangements and their implications for mitochondrial function.

Structural dynamics of mitochondrial ATP synthase in Chlamydomonas reinhardtii revealed by in situ CryoET

The paper uses cryo-ET to study mitochondrial ATP synthase dynamics in Chlamydomonas reinhardtii, revealing conformational states and movement patterns of its components.

The distortion-push mechanism for the γ-subunit rotation in F1-ATPase

The paper investigates the γ-subunit rotation mechanism in F1-ATPase using cryo-EM structures and molecular dynamics simulations, proposing the distortion-push mechanism.

Engineering of ATP synthase for enhancement of proton-to-ATP ratio

This study engineered ATP synthase to increase the H+/ATP ratio beyond natural limits, enabling ATP synthesis at lower proton motive force.

Novel If1 mechanism preventing ATP hydrolysis by the ATP synthase subcomplex in Saccharomyces cerevisiae

This study investigates the role of If1 inhibitory peptides in Saccharomyces cerevisiae, showing they prevent ATP hydrolysis under glyco-oxidative stress and stabilize the F1 subcomplex.

Rotation-direction-dependent regulation of ATPase inhibitory factor 1 for mitochondrial ATP synthase from atomistic simulation

This paper uses molecular dynamics simulations to show how rotation direction affects IF1's binding to ATP synthase, revealing atomic mechanisms for IF1 ejection and inhibition.

Interaction of yeast V-ATPase with TLDc protein Rtc5p

This study investigates the role of Rtc5p in V-ATPase assembly, showing it promotes holo V-ATPase formation in vitro and binds the V1-B subunit, unlike its counterpart Oxr1p.

Conduction pathway for potassium through the E. coli pump KdpFABC

The paper investigates the conduction pathway of potassium through the E. coli KdpFABC pump using cryo-EM and biochemical assays, revealing a tunnel for K+ transport and a low-affinity release site in KdpB.

ATP synthesis of Enterococcus hirae V-ATPase driven by sodium motive force

This study demonstrates that Enterococcus hirae V-ATPase can synthesize ATP using sodium motive force, showing reversible reactions and a Na+/ATP ratio consistent with structural symmetry.

Hydrogenase-driven ATP synthesis from air

This paper demonstrates that the hydrogenase Huc from Mycobacterium smegmatis can drive ATP synthesis from air, providing a traceless energy source for bacterial survival during nutrient starvation.

Structure of a Ca2+ bound phosphoenzyme intermediate in the inward-to-outward transition of Ca2+-ATPase 1 from Listeria monocytogenes

The paper presents a cryo-EM structure of a Ca2+-bound intermediate in the Ca2+-ATPase 1 from Listeria monocytogenes, revealing insights into the inward-to-outward transition mechanism.

Dimeric assembly of F1-like ATPase for the gliding motility of Mycoplasma

The paper presents a 3.2 Å resolution cryo-EM structure of a dimeric F1-like ATPase complex in Mycoplasma mobile, revealing its role in gliding motility through conserved rotary mechanisms.

High-throughput algorithm predicts F-Type ATP synthase rotor ring stoichiometries of 8 to 27 protomers

The paper presents a high-throughput algorithm using AlphaFold to predict F-Type ATP synthase rotor ring stoichiometries from 8 to 27 protomers, validated with molecular dynamics simulations.

Maturation stage-specific V-ATPase disassembly can explain neutral pH of lysosome-related organelles

The paper shows that mature lysosome-related organelles in Tetrahymena thermophila are non-acidic due to V-ATPase disassembly, while immature ones are acidified.

Monoclonal Nanobodies Alter the Activity and Assembly of the Yeast Vacuolar H+-ATPase

This study characterizes monoclonal nanobodies that modulate yeast V-ATPase activity and assembly, showing their potential for therapeutic applications.

Rapid kinetics of H+ transport by membrane pyrophosphatase: evidence for a direct-coupling mechanism

The paper measures H+ transport kinetics in mPPase using a stopped-flow assay and finds that hydrolysis, not pyrophosphate binding, directly couples to H+ transport.

Profiling of yeast Saccharomyces cerevisiae mitochondrial AMPylome reveals a regulation of ATP synthase coupling trough subunit delta

The paper identifies AMPylated mitochondrial proteins in yeast, revealing a role for AMPylation in regulating ATP synthase activity and OXPHOS coupling.

Structure of yeast RAVE bound to a partial V1 complex

The paper determines the structure of yeast RAVE bound to a partial V1 complex using cryo-EM, revealing its role in facilitating V1-VO reassociation.

The Toxoplasma gondii homolog of ATPase inhibitory factor 1 is critical for mitochondrial cristae maintenance and stress response

This study identifies TgIF1's role in mitochondrial cristae maintenance and stress response in Toxoplasma gondii, showing its importance under hypoxia and oxidative stress.

The molecular structure of an axle-less F1-ATPase

The paper solves the cryo-EM structure of an axle-less F1-ATPase and shows that the complete γ subunit is important for efficient ATP binding.

Energetic requirements and mechanistic plasticity in Msp1-mediated substrate extraction from lipid bilayers

This study investigates how Msp1 uses ATP hydrolysis to extract transmembrane helices from lipid bilayers, finding a non-linear relationship between ATP hydrolysis and extraction efficiency.

In situ structure and rotary states of mitochondrial ATP synthase in whole cells

The paper determines the in situ structure of mitochondrial ATP synthase in Polytomella algae using electron cryo-tomography, revealing six rotary positions and 21 substates of the F1 head.

Six states of Enterococcus hirae V-type ATPase reveals non-uniform rotor rotation during turnover

The paper identifies six states of Enterococcus hirae V-ATPase using cryo-EM, revealing non-uniform rotor rotation during turnover.

Inhibited KdpFABC resides in an E1 off-cycle state

This study identifies a new inhibited conformation of KdpFABC, termed E1-P tight, resulting from KdpBS162 phosphorylation, which represents an off-cycle state in P-type ATPases.

An ancestral interaction module promotes oligomerisation in divergent mitochondrial ATP synthases

The paper reports cryo-EM structures of the ATP synthase dimer from Trypanosoma brucei, identifying an ancestral subunit-g/e module critical for oligomerization.

A novel and specific regulator of neuronal V-ATPase in Drosophila

This study identifies CG31030 as a specific regulator of neuronal V-ATPase in Drosophila, essential for synaptic vesicle acidification and neurotransmitter release.

Loss of a subunit of vacuolar ATPase identifies unexpected biological signatures of reduced organelle acidification in vivo

This study shows that loss of atp6v1e1b in zebrafish causes multisystemic defects linked to reduced organelle acidification, revealing new biological impacts of v-ATPase dysfunction.

The SERCA residue Glu340 mediates inter-domain communication that guides Ca2+ transport

The paper investigates the role of Glu340 in SERCA's inter-domain communication, showing that the E340A mutation slows Ca2+ binding kinetics and alters structural dynamics.

Mitochondrial ATP Synthase Trafficking along Microtubules to Cell Surface Depends on KIF5B and DRP1

This study identifies KIF5B and DRP1 as key factors in mitochondrial ATP synthase trafficking to the cell surface via microtubules in cancer cells.