ATP Synthase and ATPases Research

Evolution of membrane rotary ATP synthases: A trade-off between the net productivity and mechanistic stability?

This study investigates the evolution of rotary ATP synthases, finding that efficiency gains in F-ATPases came at the cost of mechanistic stability, while lateral gene transfers contributed to the diversification of A-ATPases.

Cryo-EM structure of the Arabidopsis thaliana V-type ATPase

The paper determines the Cryo-EM structure of the Arabidopsis thaliana V-type ATPase, revealing its catalytic cycle and organelle-specific interactions with TLDc proteins.

A 13-subunit c-ring in the Chlamydomonas chloroplast ATP synthase lowers the H⁺/ATP cost of carbon fixation

This paper reports a 13-subunit c-ring in the Chlamydomonas chloroplast ATP synthase, which lowers the H+/ATP cost of carbon fixation compared to the 14-subunit structure in spinach.

Nanoscale spatial confinement of proton flux by cardiolipin drives high-speed lateral proton transport in mitochondria

This paper models proton transport in mitochondria, showing cardiolipin creates a nanoscale barrier that confines protons, enabling efficient lateral transport for ATP synthesis.

Engineering a highly active thermophilic F1-ATPase by homolog-guided exploration and machine-learning-assisted prioritization

This paper engineers a thermophilic F1-ATPase with higher catalytic activity using homolog-guided mutation identification and machine learning, finding a 1.8-fold increase in rotation rate.

Comparative electrostatic mapping of ATP synthase reveals species-dependent axial asymmetry

The paper compares electrostatic properties of ATP synthase across species using crystallographic and cryo-EM structures, revealing species-dependent axial asymmetry in electrostatic potential.

Evolutionary divergence V-ATPase function in macropinocytic cup remodeling

This study identifies a novel role of V-ATPase in macropinocytic cup remodeling in Entamoeba histolytica by interacting with actin and phosphatidic acid.

Many-body quantum percolation sustains ohmic proton flux through the nanoconfined Fo motor

The paper resolves a biophysical paradox by showing that quantum percolation, not classical physics, enables efficient proton transport through the Fo motor under extreme nanoconfinement.

Na+-translocating oxaloacetate decarboxylase from Vibrio cholerae: the functional tautomeric form of the substrate and the proton pathways in catalysis

This study identifies the keto form of oxaloacetate as the substrate for Vibrio cholerae decarboxylase and elucidates the proton transport mechanism during catalysis.

Characterisation of the new microalgal protein xATPA related to the F-type ATP synthase α subunit, from the ecosystem to the molecule

This study characterizes a novel microalgal protein xATPA, showing its environmental prevalence and functional role in plastidial physiology without ATP synthase activity.

A planar dimer of bovine ATP synthase

The paper identifies a planar dimeric form of bovine ATP synthase with minimal membrane bending, contrasting with canonical dimers that induce significant curvature.

Structure Bioinformatics of Eight Human ATP Synthase Fo Subunits and Their AlphaFold3-Predicted Water-Soluble QTY Analogs

This paper applies the QTY code to eight human ATP synthase Fo subunits to reduce hydrophobicity while preserving structure, showing reduced hydrophobic patches and similar overall folds.

A Minimal Chemo-mechanical Markov Model for Rotary Catalysis of F1-ATPase

The paper develops a minimal chemo-mechanical Markov model for F1-ATPase rotary catalysis, showing that four beta-subunit conformations are required and reconciling the bi-site versus tri-site controversy.

Bedaquiline inhibits the ATP synthase leak channel and prevents glutamate-induced neuronal death

This paper shows that bedaquiline inhibits the ATP synthase c-subunit leak channel and prevents glutamate-induced neuronal death in primary hippocampal neurons.

The Lower γ Region Ensures Unidirectional Rotation and Torque Generation in the Latter Half of the 80° Substep of F1-ATPase

This paper investigates the role of the lower γ region in F1-ATPase rotation, finding it contributes to unidirectional rotation and torque generation specifically in the latter half of the 0-80° step.

Proteolytic Processing of the Unique F1-ATPase Subunit α in Procyclic Trypanosoma brucei

The paper identifies proteolytic processing of the F1-ATPase subunit α in Trypanosoma brucei, showing that cleavage occurs in the cytosol before mitochondrial import.

Coupling of ATP and PMF in Escherichia coli is determined by growth conditions

This study shows that ATP and PMF coupling in E. coli depends on growth conditions, with PMF being essential for growth regardless of ATP synthesis function.

Lipids are essential for potassium transport by KdpFABC from E. coli

This study uses cryo-EM to capture high-resolution structures of the KdpFABC potassium pump in E. coli during active transport, revealing lipid interactions and functional roles in potassium movement.

F1F0-ATP-synthase subunit b head-to-head interactions shape intracellular membranes in Escherichia coli

The paper investigates how overproduction of F1F0-ATP synthase subunit b in E. coli shapes intracellular membranes through its C-terminal domain, revealing a novel mechanism for membrane structuring and adaptation to membrane stress.

Computational Synthetic Inner Membrane Reveals Cardiolipin-Leak Control of ATP Output

This paper introduces a computational model of the inner mitochondrial membrane that identifies cardiolipin and leak as key regulators of ATP output through parameter sweeps and sensitivity analysis.

Supercharging the calcium pump: Identification of an activation hotspot on SERCA by cryo-EM.

This paper identifies an activation hotspot on SERCA using cryo-EM structures of SERCA bound to two activators, revealing structural insights for rational drug design.

RNAseK is vital for epithelial proton pump activity in Drosophila melanogaster

This study identifies RNAseK as essential for epithelial proton pump activity in Drosophila melanogaster, showing its role in tissue morphology, transport, and survival.

Long-term single-molecule Ca2+ flux recordings reveal mode-switching regulation of Ca2+-ATPases

This study uses single-molecule recordings to show Ca2+-ATPases switch between active and inactive modes, revealing a new regulatory mechanism for Ca2+ homeostasis.

Altered dynamics of ATP synthase cause functional irregularities in senescent hiPS-cardiomyocytes

The paper investigates altered ATP synthase dynamics in senescent hiPS-cardiomyocytes, linking structural changes in mitochondria to reduced enzyme activity and impaired cardiac function.

The In Situ Structure and Distribution of V-ATPase in C. elegans Apical Membrane Stacks

The paper examines the structure and distribution of V-ATPase in C. elegans apical membrane stacks using cryo-ET, showing they form clusters and acidify the cuticle.

Uncovering the link between ATP synthase and the TCA cycle by crosslinking mass spectrometry

This study identifies a novel link between ATP synthase and the TCA cycle using crosslinking mass spectrometry, showing how ATP synthase interacts with TCA cycle enzymes and adapts under mitochondrial dysfunction.

Mammalian RAVE couples V-ATPase assembly to organelle acidification and function

This study identifies mRAVE as a mammalian V-ATPase assembly factor that links organelle acidification to function and nutrient signaling.

Conformational dynamics and asymmetry in multimodal inhibition of membrane-bound pyrophosphatases

This study investigates the inhibitory effects of bisphosphonates on Thermotoga maritima mPPase, revealing conformational asymmetry and symmetry-breaking during catalysis.

Functional and Structural Characterization of F1-ATPase with common ancestral core domains in stator ring

The paper characterizes the functional and structural properties of an ancestral F1-ATPase, revealing it was originally a 6-stepper that diversified into 3-, 6-, and 9-step forms.

Structural Basis for Efficient Fo Motor Rotation Revealed by MCMD simulation and Structural Analysis

This study uses hybrid simulations to show that side chain flexibility and angular mismatch enhance Fo motor rotation efficiency, with conserved structural features across species.