DNA Repair Mechanisms

This study examines how the K845N mutation in LIG1 affects nick binding and sealing using biochemical, structural, and single-molecule approaches, revealing reduced ligation efficiency and altered binding dynamics.

This study shows that BIR is activated to repair R-loop-associated double-strand breaks in SETX-deficient cells, leading to hyper-recombination and synthetic lethality with PIF1, RAD52, and XPF.

This study investigates the polarity of RNA Polymerase II as a roadblock to DNA replication forks, showing that head-on collisions lead to stable binding and R-loop formation, with TFIIS facilitating hybrid removal.

This study shows DNA damage checkpoints balance a tradeoff between reducing arrest failure in diploid and polyploid cells, with optimal checkpoint strengths being suboptimal for each failure type.

This study identifies the role of the SLX4-XPF-ERCC1 nuclease complex in repairing alcohol-induced DNA crosslinks by making two precise incisions around the lesion.

This paper identifies synthetic genetic interactions of the hrq1{Delta}N allele in yeast, revealing distinct roles of the Hrq1 NTD in genome integrity pathways.

This study shows that synaptonemal complex components protect double-Holliday junctions to ensure crossover recombination during meiosis.

This study investigates the phenotypic outcomes of reduced MRE11-ATLD1 expression in mice, revealing anemia, bone marrow failure, and impaired lymphocyte development.

This study shows that KAT5 acetylates PC4 at K80 to enhance DNA repair, and this acetylation is critical for DNA damage response.

This study identifies phosphorylation of BRC-1/BRD-1 in response to DNA damage as critical for maintaining germline integrity in C. elegans by preventing meiotic DSB accumulation and chromosome breakage.

This study identifies SYCP1 as a chromatin-bound regulator in breast cancer that promotes DNA repair, transcription, and drug resistance, with clinical implications for prognosis and therapy.

This study reconstitutes sister chromatid cohesion in budding yeast, revealing two pathways for cohesin recruitment and a two-step mechanism for co-entrapment.

This study directly visualizes replisome-cohesin interactions and shows that replisomes pass through cohesin rings, establishing cohesion between sister chromatids.

This study tests the effects of nucleoside analogues on fission yeast with DNA replication checkpoint mutations, revealing sensitivity patterns and potential clinical implications.

The paper investigates how abasic sites on leading and lagging strands affect replisome progression and bypass mechanisms in Xenopus egg extracts, showing distinct stalling effects but similar reliance on translesion synthesis.

The paper identifies Rad51 interaction motifs in Rad54 and Hed1, and solves their CryoEM structures to explain their roles in regulating Rad51 activity during mitosis and meiosis.

The paper shows that the RecBC complex protects nascent DNA from degradation by ExoI during lesion bypass, ensuring efficient repair of single-stranded DNA gaps.

This study shows DNA polymerase α-primase can bypass oxidized or hydrolyzed template nucleotides, explaining why it lacks a proofreading exonuclease.

This study investigates the role of CSB protein in genome maintenance under oxidative stress, finding that CSB-deficient cells show resistance to acute stress but accelerated senescence under chronic conditions.

The paper presents the structure of Sld3CBD-Cdc45 complex and reveals interactions and conformational changes involved in Cdc45 recruitment for CMG complex formation.

This study identifies a novel role for Gen nuclease in promoting polyploid cell proliferation unrelated to DNA repair, by influencing DNA replication rates in non-physiological polyploidy.

This study identifies DNA binding preferences of BRCA1 domains, showing DBD1 binds dsDNA and DBD2/BRCT bind ssDNA and G4, linking them to HR and telomere regulation.

This study shows WRN and WRNIP1 ATPases enhance the fidelity of Y-family DNA polymerase translesion synthesis by preventing nucleotide misincorporations.

The paper tracks DNA repair dynamics in yeast using high-throughput methods, revealing chromatin reconfiguration linked to single- and double-strand breaks.

This study identifies condensin as a regulator of meiotic chromosome axis remodeling near crossover sites in yeast, showing it promotes cohesin removal and affects chromosome segregation.

This study shows that APE1's Asn174 residue stabilizes the AP-site through hydrogen bonding and electrostatic interactions, which are essential for catalytic activity.

This study shows that SETD6 methylates RAD18, affecting its localization and reducing DNA damage.

The paper shows donor transcription suppresses D-loops in cis, promoting genome stability in S. cerevisiae.

This study shows NSUN2 facilitates DICER cleavage of DARTs-associated R-loops to promote DNA repair after damage.

The paper presents the cryoEM structure of a RAD51 displacement loop, revealing the mechanism of strand exchange during homologous recombination.