Trypanosoma species research and implications

Trypanosoma cruzi Has 32 Chromosomes: A Telomere-to-Telomere Assembly Defines Its Karyotype.

The paper presents a telomere-to-telomere assembly of the Trypanosoma cruzi genome, revealing a 32-chromosome karyotype with conserved structures and identifying a tetraploid chromosome 16.

Flagellar pocket collar biogenesis: Cytoskeletal organization and novel structures in a unicellular parasite

This study uses ultrastructure expansion microscopy to investigate FPC biogenesis in Trypanosoma brucei, identifying novel structures and tracking key proteins during cell division.

Cas10 based 7SL-sRNA diagnostic for the detection of active trypanosomiasis

This paper develops a Cas10-based lateral flow assay for detecting 7SL-sRNA in cattle with active trypanosomiasis, showing high sensitivity and specificity during chronic infection phases.

A SHERLOCK toolbox for eco-epidemiological surveillance of African trypanosomes in domestic pigs from Western Africa.

The paper adapts the SHERLOCK CRISPR-based detection tool for trypanosome species in pigs to improve AAT diagnostics and identify human-infectious trypanosome reservoirs.

Elucidating the Transport Mechanisms and Metabolic Roles of Serine, Threonine, and Glycine in Trypanosoma cruzi

This study investigates the transport and metabolic roles of serine, threonine, and glycine in Trypanosoma cruzi, showing their importance in energy metabolism and stress response.

A Ras-like GTPase in African trypanosomes

This study investigates TbRlp, a Ras-like GTPase in Trypanosoma brucei, showing its role in proliferation and localization to the late endosomal system.

Trypanosoma brucei cattle infections contain cryptic transmission-adapted bloodstream forms at low parasitaemia

This study identifies cryptic stumpy-like T. brucei forms in cattle with low parasitaemia, challenging rodent-based assumptions about stumpy form differentiation.

Functional diversification of the BBSome: Insights from honey bee trypanosomatid parasite, Lotmaria passim

This study investigates the BBSome's role in Lotmaria passim, showing its importance for lipid raft integrity and infection efficiency, with functional differences compared to Leishmania mexicana.

Extrafollicular activation of B cells contributes to the long-term survival of CD28KO mice infected with Sylvio X10/4Trypanosoma cruzi parasites

This study shows that extrafollicular B cell responses in CD28KO mice infected with Sylvio X10/4 Trypanosoma cruzi contribute to long-term survival despite higher parasitemia and inflammation.

Predicting mammal species suitable for Chagas disease spread

The paper predicts mammal species that could act as reservoirs for Chagas disease by analyzing trait data and environmental factors.

Serial "deep-sampling" PCR of fragmented DNA reveals the wide range of Trypanosoma cruzi burden among chronically infected hosts and allows accurate monitoring of parasite load following treatment

This study introduces deep-sampling PCR to detect and quantify T. cruzi parasite load in chronically infected hosts, revealing large variations in parasite levels and enabling treatment monitoring.

Ultra-high throughput in vitro translation assay for identification of Trypanosoma cruzi-specific protein synthesis inhibitors

This paper develops an ultra-high throughput in vitro translation assay for identifying T. cruzi-specific protein synthesis inhibitors and identifies quinazoline compounds that inhibit translation in both the assay and live parasites.

A high-throughput protein tagging toolkit that retains endogenous UTRs for studying gene regulation in Kinetoplastids.

The paper introduces a CRISPR/Cas9-based protein tagging system for Kinetoplastids that preserves endogenous regulatory elements and demonstrates its utility in studying gene regulation.

Title Longitudinal Observational (single cohort) Study on the Causes of Trypanocide Failure in cases of African Animal Trypanosomosis in Cattle Near Wildlife Protected Areas of Northern Tanzania

This study identifies factors contributing to trypanocide failure in cattle with African animal trypanosomosis in Tanzania, finding drug quality, improper use, and resistance as key issues.

Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability in Trypanosoma brucei

This study uses Nanopore sequencing to show that DNA replication compartmentalization in Trypanosoma brucei affects genome stability and instability by influencing VSG gene recombination and chromosome stability.

Reconstructing Sordida subcomplex (Hemiptera, Reduviidae, Triatominae) phylogeny across species distribution range

This study uses molecular and chromosomal analyses to reveal genetic diversity in the Sordida subcomplex, suggesting six putative species instead of three.

TelAP2 links TelAP1 to the telomere complex in Trypanosoma brucei

The paper identifies TelAP2 as an interacting partner of TelAP1 in the telomere complex of Trypanosoma brucei and shows that TelAP1 requires this interaction to associate with telomeric proteins.

Mechanisms of life cycle simplification in African trypanosomes

This study identifies molecular changes and reversible steps in the loss of stumpy transmission in monomorphic trypanosomes using genomic analysis and laboratory selection.

Molecular characterization of the permanent outer-inner membrane contact site of the mitochondrial genome segregation complex in trypanosomes

This study identifies the molecular architecture of the TAC60-p166 contact site in Trypanosoma brucei, showing hydrophobic interactions drive its formation rather than electrostatic forces.

Rab2B promotes chaperonin-mediated actin folding and prevents developmental transcription reprogramming and quiescence in trypanosomes

This study shows Rab2B and actin folding via CCT/TRiC prevent developmental differentiation and quiescence in trypanosomes.

Transcriptomic analysis of benznidazole-resistant Trypanosoma cruzi clone reveals nitroreductase I-independent resistance mechanisms

This study identifies nitroreductase I-independent resistance mechanisms in benznidazole-resistant Trypanosoma cruzi clones through transcriptomic analysis.

Somy evolution in the honey bee infecting trypanosomatid parasite, Lotmaria passim

This paper updates the genome assembly and annotation of Lotmaria passim, revealing chromosomal duplications and aneuploidy, and provides a reference for studying its adaptation to the honey bee gut and potential treatments for pollinator infections.

TropD-Detector: A CRISPR/LbCas12a-Based system for rapid and sensitive screening of Trypanosoma cruzi in Chagas vectors and reservoirs

The paper develops a CRISPR/LbCas12a-based system for detecting Trypanosoma cruzi in vectors and reservoirs, achieving high sensitivity and introducing a fluorescent detection prototype.

Genome report: First whole genome sequence of Triatoma sanguisuga (Le Conte, 1855), vector of Chagas disease

The paper reports the first whole genome sequence of Triatoma sanguisuga, a Chagas disease vector, using long-read sequencing and provides genomic insights for public health research.

GENETIC DIVERSITY OF T. B. RHODESIENSE SERUM RESISTANCE ASSOCIATED GENE IN MALAWIAN ISOLATES

The paper investigates the genetic diversity of the SRA gene in T. b. rhodesiense isolates and APOL-1 genotypes in Malawian r-HAT patients, finding heterozygosity in SRA and severe r-HAT cases in APOL-1 G2 individuals.

Allosteric inhibition of trypanosomatid pyruvate kinases by a camelid single-domain antibody

This study identifies a camelid single-domain antibody that allosterically inhibits trypanosomatid pyruvate kinases, reducing parasite growth.

KREH2 helicase represses ND7 mRNA editing in procyclic-stage Trypanosoma brucei by opposite modulation of canonical and moonlighting gRNA utilization creating a proposed mRNA structure

The paper identifies KREH2 helicase as a repressor of ND7 mRNA editing in T. brucei by modulating canonical and moonlighting gRNA utilization, revealing its dual role in developmental editing control.

Trypanosome infection rate and blood-feeding patterns of tsetse fly in an area with a recent human African trypanosomiasis outbreak in Southwest Ethiopia

The study assesses trypanosome infection rates and tsetse fly feeding patterns in Ethiopia, finding 17.1% infection rate with T. congolense dominant, and higher infection rates with combined cow urine and acetone baits.

Generation of a bloodstream form Trypanosoma brucei double glycosyltransferase null mutant competent in receptor-mediated endocytosis of transferrin

This paper creates double null mutants of TbGT8 and TbGT10 in Trypanosoma brucei, showing reduced pNAL synthesis but normal TfR function and parasite viability.

Mono-allelic epigenetic regulation of bi-directional silencing of RNA Polymerase II polycistronic transcription initiation in Trypanosoma brucei.

The paper shows that epigenetic mechanisms, including base J modification, regulate mono-allelic transcriptional silencing of adjacent polycistronic gene clusters in Trypanosoma brucei.