Zebrafish Biomedical Research Applications

PyZebrascope: an open-source platform for brain-wide neural activity imaging in zebrafish

PyZebrascope provides an open-source platform for whole-brain neural activity imaging in zebrafish using light-sheet microscopy, enabling high-throughput and stable data acquisition.

Long-Term Imaging of Living Adult Zebrafish

This paper introduces a method for long-term imaging of live adult zebrafish using a 3D printed chamber, enabling nearly day-long observation of neutrophil recruitment in transgenic fish.

Anatomy and Development of the Pectoral Fin Vascular Network in the Zebrafish

The paper documents the stepwise development of the zebrafish pectoral fin vascular network using high-resolution imaging, revealing a stereotyped process involving initial vascular loops and three distinct plexuses.

The neuropeptide Pth2 modulates social behavior and anxiety in zebrafish

This study shows that the neuropeptide Pth2 in zebrafish affects anxiety and social behavior, with altered responses to stimuli and changes in social preference during development.

Osteoblasts pattern endothelium and somatosensory axons during zebrafish caudal fin organogenesis

Voltage imaging identifies spinal circuits that modulate locomotor adaptation in zebrafish

The paper uses voltage imaging to identify spinal circuits in zebrafish that modulate locomotor adaptation by characterizing a novel sub-population of V3 neurons.

The transcriptome of regenerating zebrafish scales identifies genes involved in human bone disease

The paper identifies genes involved in human bone disease by analyzing transcriptomic changes during zebrafish scale regeneration.

Single-cell resolution of MET and EMT programs during zebrafish fin regeneration

This study uses scRNA-seq to analyze MET and EMT programs in zebrafish fin regeneration, showing co-expression of EMT markers in dedifferentiating and differentiating osteoblastic cells.

FRaeppli, a multispectral imaging toolbox for cell tracing and dense tissue analysis in zebrafish

FRaeppli enables multispectral imaging for cell tracing and tissue analysis in zebrafish, revealing new canalicular topologies in hepatocytes.

Contingent-behavior assay to study the neurogenetics of addiction shows zebrafish preference for alcohol is biphasic

The paper develops a zebrafish assay to study alcohol preference, finding a biphasic response where low alcohol concentrations are attractive and high concentrations are avoided.

3D quantification of zebrafish cerebrovascular architecture by automated image analysis of light sheet fluorescence microscopy datasets

This paper presents a 3D image analysis pipeline for quantifying zebrafish brain vasculature, showing it is robust and provides novel insights into vascular biology.

Colony stimulating factor 1 signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands

This study investigates the roles of Csf1rs and their ligands in zebrafish myelopoiesis, revealing distinct functions in macrophage and granulocyte differentiation.

Heterogeneity and genomic loci of ubiquitous Cre reporter transgenes in zebrafish

The paper compares zebrafish Switch reporter transgenes to identify those with optimal recombination efficiency and expression patterns for lineage labeling.

Wasp controls oriented migration of endothelial cells to achieve functional vascular patterning

The paper shows that WASp regulates endothelial cell migration directionality in zebrafish, leading to proper vascular patterning and preventing vascular malformations.

A chemical screen based on an interruption of zebrafish gastrulation identifies the HTR2C inhibitor Pizotifen as a suppressor of EMT-mediated metastasis

The paper identifies Pizotifen as an anti-metastasis drug by screening chemicals that disrupt zebrafish gastrulation and show it suppresses EMT-mediated metastasis through HTR2C inhibition.

Novel pre-clinical model for CDKL5 Deficiency Disorder

This study develops a zebrafish model for CDKL5 Deficiency Disorder to investigate disease mechanisms and screen for therapies.

ZAF -- The First Open Source Fully Automated Feeder for Aquatic Facilities

The paper introduces ZAF, an open-source automated feeding system for aquatic facilities, which provides standardized food distribution and is adaptable to various laboratory environments.

AZBA: A 3D Adult Zebrafish Brain Atlas for the Digital Age

The paper introduces AZBA, a 3D adult zebrafish brain atlas created using tissue clearing and imaging techniques, providing a detailed segmentation of over 200 neuroanatomical regions.

Precise visuomotor transformations underlying collective behavior in larval zebrafish

The paper studies how larval zebrafish transform visual inputs from neighbors into movement decisions during schooling behavior, finding that young larvae use averaging and winner-take-all strategies, while mature fish incorporate attraction to low retinal clutter.

Q-STARZ: Quantitative Spatial and Temporal Assessment of Regulatory element activity in Zebrafish

Q-STARZ assesses spatial and temporal activity of wild-type and mutant human CRE alleles in zebrafish using live imaging and transgenic reporters.

Functional characterisation of single nucleotide variants of the psychiatric risk gene cacna1c in the zebrafish

This study investigates the functional effects of two CACNA1C mutations in zebrafish, showing altered mRNA levels, neurotransmitter profiles, and behavioral and electrophysiological changes linked to psychiatric disorders.

Central nervous system hypomyelination disrupts axonal conduction and behaviour in larval zebrafish

This study shows that hypomyelination in zebrafish larvae disrupts axonal conduction and behavior, linking myelination to neural circuit function.

5-HT2C agonists and antagonists block different components of behavioral responses to potential, distal, and proximal threat in zebrafish

The paper investigates the role of 5-HT2C receptor agonists and antagonists in modulating zebrafish behavioral responses to different types of threat.

How do zebrafish respond to MK-801 and amphetamine? Relevance for assessing schizophrenia-relevant endophenotypes in alternative model organisms

This study examines the effects of MK-801 and amphetamine on zebrafish behavior and oxidative stress, finding MK-801 disrupts social interaction and locomotion, while amphetamine increases lipid peroxidation.

Knock-in tagging in zebrafish facilitated by insertion into non-coding regions

The paper describes a method to generate endogenously tagged zebrafish knock-in lines by inserting fluorescent markers into non-coding regions of genes.

Modelling spinal locomotor circuits for movements in developing zebrafish

This study builds and simulates spinal circuit models in developing zebrafish to understand how locomotor movements transition from coiling to swimming.

Identification of Maternal-Effect Genes in Zebrafish using Maternal Crispants

This study identifies and characterizes maternal-effect genes in zebrafish using CRISPR-Cas9 to generate maternal crispants, revealing two new genes and validating known ones.

zmiz1a zebrafish mutants have defective erythropoiesis, altered expression of autophagy genes, and a deficient response to vitamin D

Affordable and Effective Optokinetic Response Methods to Assess Visual Acuity and Contrast Sensitivity in Larval to Juvenile Zebrafish

This paper introduces 2D- and 3D-printed striped drums for measuring optokinetic response, contrast sensitivity, and visual acuity in larval zebrafish, showing equivalent responses to standard methods.

Identification of Subpallial Neuronal Populations Across Zebrafish Larval Stages that Express Molecular Markers for the Striatum

The paper identifies two subpallial neuronal populations in larval zebrafish that express molecular markers associated with mammalian striatal pathways and show developmental increases.