Zebrafish Biomedical Research Applications

Decynium-22 affects behavior in the zebrafish light/dark test

This study shows that Decynium-22 increases anxiety-like behavior in zebrafish by affecting monoamine levels and the uptake2 system, suggesting a role for this system in defensive behavior.

The effect of absent blood flow on the zebrafish cerebral and trunk vasculature

This study quantifies how absent blood flow affects vascular development in zebrafish cerebral and trunk vasculature, finding more severe effects in the brain.

Conserved and context-dependent roles for Pdgfrb signaling during zebrafish vascular mural cell development

The paper shows that Pdgfrb signaling is essential for mural cell development in zebrafish, similar to mice, and identifies context-dependent roles in vascular structures.

The formin Fmn2 is required for the development of an excitatory interneuron module in the zebrafish acoustic startle circuit

This study identifies Fmn2's role in zebrafish neural circuit development, specifically in the formation of spiral fiber neurons critical for the acoustic startle response.

Using zebrafish to elucidate glial-vascular interactions during CNS development

This study uses zebrafish to investigate glial-vascular interactions during CNS development, showing conserved lineage-specific roles in vessel maintenance and formation.

The inducible lac operator-repressor system is functional in zebrafish cells

The paper demonstrates that the lac operator-repressor system functions in zebrafish cells to modulate gene expression.

A Transgenic System for Targeted Ablation of Reproductive and Maternal-Effect genes

The paper develops reverse genetic systems to identify essential maternal-effect genes and tests them by disrupting kif5Ba and bucky ball.

Rapid generation of pigment free, immobile zebrafish embryos and larvae in any genetic background using CRISPR-Cas9 dgRNPs

This paper demonstrates the use of CRISPR-Cas9 dgRNPs to generate pigment-free, immobile zebrafish embryos and larvae across genetic backgrounds, enhancing live imaging capabilities.

A forward genetic screen identifies Dolk as a regulator of startle magnitude through the potassium channel subunit Kv1.1

This study identifies eight genes, including Dolk and Kv1.1, that regulate zebrafish startle behavior through a common genetic pathway, with Dolk affecting Kv1.1 protein localization.

Understanding the complexity of Epimorphic Regeneration in zebrafish: A Transcriptomic and Proteomic approach.

This study identifies 1408 genes and 661 proteins differentially regulated during zebrafish caudal fin regeneration using transcriptomics and proteomics.

Minimally invasive brain injections for viral-mediated transgenesis: New tools for behavioral genetics in sticklebacks

This paper introduces a minimally invasive brain injection technique for viral-mediated transgenesis in sticklebacks to study genetic effects on behavior.

Phenotypic screening using synthetic CRISPR gRNAs reveals pro-regenerative genes in spinal cord injury

The paper uses synthetic CRISPR gRNAs and in vivo pre-screening to identify pro-regenerative genes in spinal cord injury, finding that tgfb1a promotes regeneration by controlling inflammation.

Algorithms underlying flexible phototaxis in larval zebrafish

This study shows larval zebrafish use a homeostatic algorithm to adjust their phototactic behavior based on luminance history and environmental changes.

BonZeb: Open-source, modular software tools for high-resolution zebrafish tracking and analysis

BonZeb provides modular tools for high-resolution zebrafish tracking with support for various experimental configurations and analysis workflows.

Endothelial struts, a mechanism to generate large lumenized blood vessels de novo

This paper describes a new mechanism for forming large diameter blood vessels through endothelial struts that act as scaffolds during vasculogenesis.

In vivo dissection of Rhoa function in vascular development using zebrafish

This study investigates the in vivo roles of RHOA in vascular development and integrity using zebrafish models, finding that both gain- and loss-of-function lead to vascular dysfunction.

Efficient CRISPR/Cas9 mutagenesis for neurobehavioral screening in adult zebrafish

This study develops an efficient CRISPR/Cas9 pipeline for identifying genes involved in spinal cord repair in adult zebrafish through mutagenesis and behavioral screening.

A CRISPR/Cas9 vector system for neutrophil-specific gene disruption in zebrafish

This paper develops a CRISPR/Cas9 system for neutrophil-specific gene disruption in zebrafish and demonstrates its utility in studying gene function.

Behavioral effects of developmental exposure to JWH-018 in wild type and disrupted in schizophrenia 1 (disc1) mutant zebrafish

This study examines the behavioral effects of developmental JWH-018 exposure in zebrafish and finds no moderation by disc1 mutations in anxiety-like behaviors.

Dlc1 controls cardio-vascular development downstream of Vegfa/Kdrl/Nrp1 signaling in the zebrafish embryo.

This study shows that dlc1 is essential for vascular and cardiac development in zebrafish embryos, functioning downstream of Vegfa/Kdrl/Nrp1 signaling and linked to congenital heart defects in humans.

TAEL 2.0: An Improved Optogenetic Expression System for Zebrafish

The paper improves the TAEL optogenetic system for zebrafish, enhancing gene expression induction while maintaining safety, and creates stable transgenic lines for broader use.

Endogenous zebrafish neural Cre drivers generated by CRISPR/Cas9 short homology directed targeted integration

This paper generates zebrafish Cre drivers by integrating a 2A-Cre transgene into proneural genes, resulting in functional Cre expression in specific cell populations.

A Customizable Low-Cost System for Massively Parallel Zebrafish Behavior Phenotyping

The paper describes a low-cost, modular system for high-throughput zebrafish behavior phenotyping, validated with mutant analysis and best practices for larval growth.

A simple and effective F0 knockout method for rapid screening of behaviour and other complex phenotypes

This paper introduces a CRISPR-Cas9 method for rapid generation of F0 biallelic knockouts in zebrafish, enabling quick screening of complex phenotypes.

Cohesin components Stag1 and Stag2 differentially influence haematopoietic mesoderm development in zebrafish embryos

This study shows that Stag1 and Stag2 differentially affect haematopoietic mesoderm development in zebrafish embryos, with stag1a and stag2b mutants showing distinct hematopoietic defects.

Postembryonic screen for mutations affecting spine development in zebrafish

This study identifies mutations affecting spine development in zebrafish, revealing new candidate genes for human spine disorders like scoliosis.

Zebrafish Kit ligands cooperate with erythropoietin to promote erythroid cell expansion

The paper shows that Kit ligands cooperate with erythropoietin to promote erythroid cell expansion in zebrafish and reports ex vivo suspension cultures of zebrafish hematopoietic progenitor cells.

Adaptive cell invasion maintains organ homeostasis

The paper identifies invasive skin-derived cells in zebrafish lateral line that differentiate into ionocytes, adapting to environmental ionic changes and regulating hair cell function.

Assessment of autism zebrafish mutant models using a high-throughput larval phenotyping platform

The paper develops a high-throughput phenotyping protocol for zebrafish larvae to assess autism-related mutant models, identifying morphological and behavioral defects in syngap1b and slc7a5 mutants.

A zebrafish functional genomics model to investigate the role of human A20 variants in vivo

This paper uses a zebrafish model to study the in vivo effects of human A20 variants, showing that specific mutations impact A20 function and disease relevance.