Barrier Structure and Function Studies

This study shows that disrupting glycogen utilization in mice causes microvascular constrictions and worsens ischemia outcomes.

The paper demonstrates that oligodendrocytes mediate axo-vascular coupling in the mouse optic nerve by regulating vascular dilation in response to axonal activity.

The paper identifies Wnt-β-catenin signaling repression as key to arachnoid barrier cell specification in mouse meninges and shows its role in preventing pathogen entry during development.

This study shows that pericyte loss in aged brains leads to capillary dilation, flow heterogeneity, and structural disruption, highlighting pericyte remodeling as a therapeutic target.

This study uses a SARS-CoV-2 biomimetic to investigate how BBB dysregulation may lead to neuroinflammation without direct viral infection.

The paper uses two-photon microscopy to show that S1P signaling impairment causes glycocalyx loss in arterioles but not other vessel types in Apom-/- mice.

This study shows that isoflurane induces initial calcium increases then suppression in cerebrovascular endothelial cells, disrupting vascular tone during anesthesia.

This study identifies capillary pericytes as metabolic sentinels that increase blood flow in response to glucose depletion through KATP channel activation.

This study shows that tunneling nanotubes (TNTs) enable mitochondrial transfer between BBB cells, rescuing astrocytes from ischemia/reperfusion-induced apoptosis and highlighting pericytes' role in BBB preservation.

This study shows that Norrin/β-catenin signaling in mural cells increases Lama2 expression, which promotes neurovascular unit assembly and barrier maturation.

The paper identifies cell-type-specific molecular changes in cerebrovascular cells across brain regions in Alzheimer's Disease, linking them to genetic variants and potential therapeutic targets.

The paper uses single nucleus RNA sequencing to analyze brain vascular cells in Alzheimer's disease, identifying molecular changes and interactions between vascular and astrocytic cells at the blood-brain barrier.

This study identifies transcriptional and epigenetic mechanisms driving endothelial diversity across organs in mice, revealing conserved gene regulatory networks in the brain vasculature.

This study shows adropin protects against ischemic stroke by maintaining BBB integrity and reducing neuroinflammation, improving functional recovery in mice.

This study identifies age-related impairments in brain microvascular dynamics, particularly in precapillary sphincters, leading to reduced neurovascular function in aging.

The paper identifies Atp13a5 as a specific marker for CNS pericytes in mice and develops a transgenic model to study their biology.

The paper uses intravital imaging in mice to study spinal cord vascular permeability and function in real time.

The paper compares molecular and functional maturation of brain vascular cells in mice and humans during postnatal development, identifying key markers and processes in endothelial and vascular smooth muscle cells.

This study shows astrocyte plasticity maintains vascular coverage and BBB integrity by replacing ablated astrocytes, but this plasticity declines with aging.

This study shows SARS-CoV-2 spike protein induces immunoreactivity in brain pericytes without viral infection, contributing to microvasculature injury in COVID-19.

VINE-seq yields 143,793 single-nucleus transcriptomes of human brain vascular and perivascular cells; 30 of the top 45 Alzheimer's GWAS genes are expressed there, many microglial in mice.

This study characterizes human cerebrovascular cells in health and disease, identifying cell type-specific markers and revealing immune activation and BBB integrity loss in Huntington's disease.

The paper profiles vascular and perivascular cells in different brain regions to reveal molecular and cellular bases of blood-brain barrier heterogeneity.

The paper describes a high-throughput method using E. coli to identify paracellular permeability modulators by expressing TJ proteins and quantifying multicellular aggregation.

This study shows that TNAP activity in brain endothelial cells maintains barrier integrity via the ROCK pathway, and its inhibition leads to barrier dysfunction.

The paper analyzes insulin-responsive gene expression dynamics in BBB cells to identify molecular mechanisms linking insulin resistance to AD pathology.

This study shows that inhibiting VEGF signaling in AD mice reduces leucocyte adhesion in brain capillaries, increases blood flow, and improves BBB integrity.

This paper describes a protocol for isolating and culturing pia mater cells from mice and identifies novel markers for these cells.

This paper investigates how endothelial iron homeostasis affects BBB integrity through the HIF2α-Ve-cadherin pathway, identifying DFO and JP4-039 as potential therapeutic agents.