Barrier Structure and Function Studies

Atomistic Simulation of Blood Brain Barrier Permeability of Propolis Derived Natural Compounds

This study uses atomistic simulations to analyze BBB permeability of two propolis compounds, showing CAPE has favorable permeability while ARC faces significant resistance.

Barrier Function of the Vero E6 Cell Monolayer

This study demonstrates that Vero E6 cells form functional barrier layers with measurable TEER and permeability, comparable to endothelial models, and are sensitive to calcium chelation.

Paraspeckles translate microbial insult-induced inflammation into neurovascular remodeling by enhancing CYR61-FGF2 signaling via RBM14 sequestration

The paper identifies paraspeckles as mediators of microbial insult-induced neurovascular remodeling through CYR61-FGF2 signaling via RBM14 sequestration, showing therapeutic potential.

Brain defence by the extracellular matrix protein Cochlin

This study identifies Cochlin as an extracellular matrix protein produced in brain barriers that enhances immune defence against bacterial infection in zebrafish.

APOE-ϵ4-induced Fibronectin at the blood-brain barrier is a conserved pathological mediator of disrupted astrocyte-endothelia interaction in Alzheimer's disease

This paper identifies APOE-ϵ4-induced fibronectin accumulation at the blood-brain barrier as a key mediator of astrocyte-endothelial dysfunction in Alzheimer's disease, highlighting it as a potential therapeutic target.

Modulating the Novel miR-23b-Wnt/β-catenin Axis Strengthens Endothelial Barrier Properties

This study identifies miR-23b as a negative regulator of BBB integrity and shows that anti-miR-23b enhances barrier function through multiple signaling pathways and improves outcomes in a stroke model.

The Calcium Pump ATP2B1/PMCA1 Regulates CNS Vascular Development by Facilitating Norrin- and WNT7A/B-induced Frizzled4 signaling

This study identifies ATP2B1 as a regulator of Norrin- and WNT7A/B-induced Frizzled4 signaling in endothelial cells, showing its role in CNS vascular development.

Cryo-electron tomography reveals paracellular claudin-15 pores at the tight junction

The paper uses cryo-electron tomography to directly visualize paracellular claudin-15 pores in tight junctions, confirming their structure and spacing.

Mural cells protect the adult brain from hemorrhage but do not control the blood-brain barrier in developing zebrafish

This study shows that mural cells are not required for BBB function in developing zebrafish but protect against hemorrhage-induced BBB breakdown in adults.

ECgo: All-Optical Induction of Single Endothelial Cell Injury and Capillary Occlusion in the Brain

The paper introduces ECgo, an optical method to induce single endothelial cell injury and capillary occlusion in the mouse brain with high spatial precision, enabling studies of capillary repair and dysfunction.

Gαq/11 signalling counteracts endothelial dysfunction in the brain and protects cognition in aged mice

This study shows that Gαq/11 signaling in brain endothelial cells prevents endothelial dysfunction and cognitive decline in aged mice by maintaining vascular reactivity and preventing blood-brain barrier disruption.

Cerebrovascular Claudin-5 Isoform Expression Correlates with Worsened Stroke Outcomes Following Thromboembolic Stroke

This study shows that ischemic stroke causes changes in claudin-5 isoform expression in rats, which correlate with worse neurological outcomes and BBB dysfunction.

Endothelial tPA-dependent recruitment of microglia to vessels protects the blood-brain barrier after stroke in mice

This study shows that endothelial tPA helps microglia protect the blood-brain barrier after stroke in mice by reducing vascular inflammation and maintaining BBB integrity.

Endothelial Adgrl2 expression and alternative splicing controls the cerebrovasculature

The paper shows that Adgrl2 alternative splicing generates distinct isoforms in neurons and endothelial cells, with neuronal isoform expression in endothelial cells altering cerebrovascular properties and blood-brain barrier integrity.

A physiological microfluidic blood-brain-barrier model for in vitro study of nanoparticle trafficking and accumulation

The paper develops a microfluidic BBB model to study nanoparticle trafficking and accumulation, finding that ligand presentation and membrane composition, not size or stiffness, govern BBB penetration.

Angiogenic mechanisms governing the segregation of blood-brain barrier and fenestrated capillaries derived from a multipotent cerebrovascular niche

The paper identifies a multipotent vascular niche in zebrafish that generates BBB and fenestrated endothelial cells, revealing Flt4's role in their segregation through signaling with Vegfr2.

Progressive mural cell deficiencies across the lifespan in a foxf2 model of Cerebral Small Vessel Disease

This study uses zebrafish foxf2 mutants to model Cerebral Small Vessel Disease, showing progressive pericyte deficiencies and vascular abnormalities across the lifespan.

NimA promotes cell adhesion at the blood brain barrier of Drosophila nervous system

The paper identifies NimA as a glial-specific protein in Drosophila that regulates cell adhesion at the blood-brain barrier, essential for BBB integrity and neural development.

LACK OF OXYGEN AND/OR GLUCOSE DIFFERENTIALLY POTENTIATES Aβ40E22Q- AND Aβ42-INDUCED CEREBRAL ENDOTHELIAL CELL DEATH, BARRIER DYSFUNCTION AND ANGIOGENESIS IMPAIRMENT

This study shows that hypoxia and glucose deprivation enhance Aβ40E22Q- and Aβ42-induced cerebral endothelial cell death, barrier dysfunction, and angiogenesis impairment.

Panax notoginseng saponins protect the blood brain barrier against oxidative stress by activating the Akap12/PI3K/AKT/AQP1 signaling axis

This study shows that Panax notoginseng saponins protect the blood-brain barrier from oxidative stress by activating the Akap12/PI3K/AKT/AQP1 signaling pathway and modulating tight junction proteins.

Vascular Basement Membrane Laminins Modulate Functional Zonation of Cerebral Microvessels

This study shows that vascular basement membrane laminins regulate cerebral microvessel functional zonation by modulating endothelial and mural cell identity and vascular permeability.

Pericytes and Wnt signaling induce functional blood-brain barrier phenotype in human iPSC-based model

This paper develops a human iPSC-based model of the blood-brain barrier by co-culturing endothelial cells with pericytes and activating Wnt/β-catenin signaling, resulting in cells with BBB-like properties and gene expression patterns.

Building the blood-brain barrier: a scalable self-assembling 3D model of the brain microvasculature under unidirectional flow

This paper presents a scalable 3D BBB model using human cells in a microfluidic platform, showing improved barrier function with co-culture of endothelial cells, astrocytes, and pericytes.

Lipid-laden endothelial cells exhibit a transcriptomic signature linked to blood-brain barrier dysfunction, metabolic reprogramming and increased inflammation in the aging brain

This study identifies a transcriptomic signature in aged brain endothelial cells linked to lipid accumulation, BBB dysfunction, metabolic changes, and inflammation.

Glial betaPix is essential for blood vessel development in the zebrafish brain

This study identifies betaPix's role in glial cells for vascular development and blood-brain barrier formation in zebrafish embryos.

Bacterial extracellular vesicles indirectly destabilize a human stem cell-derived blood-brain barrier on-chip through pro-inflammatory stimulation of immune cells

The paper shows that bacterial extracellular vesicles disrupt a BBB on-chip model indirectly via immune cell activation, not directly through BMEC damage.

ENDOTHELIAL PROX1 INDUCES BLOOD-BRAIN BARRIER DISRUPTION IN THE CENTRAL NERVOUS SYSTEM

This study shows that endothelial Prox1 overexpression disrupts the blood-brain barrier by downregulating BBB-associated genes and Wnt/β-catenin signaling during both embryonic and postnatal stages.

The effects of Alcohol Dependence on the CSF Proteome in Mice: Evidence for Blood-Brain Barrier Dysfunction and Neuroinflammation

This study uses proteomics to compare CSF from alcohol-dependent and non-dependent mice, identifying molecular changes linked to BBB dysfunction and neuroinflammation.

Proteomic Profiling Reveals Age-Related Changes in Transporter Proteins in the Human Blood-Brain Barrier

This study uses proteomic profiling to identify age-related changes in transporter proteins in human brain microvessels, revealing alterations in BBB permeability and drug disposition.

Occludin Acts as a Dynein Adaptor Regulating Permeability and Collateral Angiogenesis

This study identifies occludin's role as a dynein adaptor regulating vascular permeability and collateral angiogenesis through phosphorylation-dependent trafficking.