Discover, rate, and verify biology preprints.

From lab to ocean: bridging swimming energetics and wild movements to understand red drum (Sciaenops ocellatus) behavior in a tidal estuary

Red drum lowered oxygen consumption behind mangrove- and oyster-mimicking bluff bodies at flows to 100 cm/s, yet telemetered fish were detected mostly in low-velocity nearshore habitat.

Short RNA chaperones promote aggregation-resistant TDP-43 conformers to mitigate neurodegeneration

Short UG-rich RNAs bind and stabilize TDP-43 RNA-recognition motifs to allosterically destabilize its prion-like domain helical region, preventing aggregation and mitigating neurodegeneration in cells and mice.

Biodistribution of mRNA vaccines in rats: Enrichment in injection site and lymph tissues and rapid clearance without tissue persistence

Three mRNA-LNP products sharing one lipid matrix show rat intramuscular biodistribution (injection site, lymph nodes, spleen) and rapid clearance, with no Spike accumulation after repeat dosing.

Interleukin-1β Drives Disease Progression in Arrhythmogenic Cardiomyopathy

Human arrhythmogenic cardiomyopathy hearts harbor a lesion niche of POSTN fibroblasts and NLRP3+ inflammatory macrophages; IL-1β neutralization in Dsg2-mutant mice reduced fibrosis, dysfunction, and ventricular ectopy.

Visual - Acoustic Thigmotaxis in Zebrafish Larvae: A High throughput NAM for Neurotoxicity Assessment

A 96-well square plate zebrafish larva thigmotaxis assay with visual and acoustic stimuli matched 24-well round plates and detected neuroactive chemicals more sensitively than locomotion.

A cross-center comparison of the relationship between matriline fragmentation, grooming cohesion, and agonistic behavior in captive rhesus macaque (Macaca mulatta) social groups

Across 105 rhesus macaque matrilines at two primate centers, family grooming cohesion drops below a mean kinship threshold of 0.16, while lower kinship continuously increases severe within-family aggression.

Effects of muscle mass on muscle force predictions in human movement

Adding distributed muscle mass to 1D Hill-type models alters predicted human leg-muscle forces by under 1% at natural size; differences grow with scale and cadence.

Predator-prey scaling laws support a suspension-feeding lifestyle in Cambrian luolishaniid lobopodians

Cambrian luolishaniid lobopodian appendage mesh spacing scales sublinearly with body length, and their predator-prey size ratios match modern suspension feeders rather than raptorial chaetognaths.

Rapid and robust sex determination from ancient enamel proteomes using protSexInferer

Using the share of AMELY-specific among all sex-specific amelogenin peptides (RAMELY), enamel proteomes are sexed consistently across four search engines, tolerating false-positive AMELY hits without manual checks.

Ancient regulatory evolution shapes individual language abilities in present-day humans

Polygenic score variants in HAQERs (Human Ancestor Quickly Evolved Regions, <0.1% of the genome) predict spoken language but not nonverbal IQ; language associations replicate in SPARK, ABCD and UK Biobank.

Senolytic treatment with fisetin reverses age-related endothelial dysfunction partially mediated by SASP factor CXCL12

Single-cell aortic transcriptomics and plasma bioassays in aged mice show that intermittent fisetin treatment clears senescent endothelial cells and reverses endothelial dysfunction partially driven by circulating CXCL12.

An integrated single cell and spatial omics atlas of human prenatal development

Human Developmental Cell Atlas integrates ~4.6 million cells/nuclei from 4-22 post-conception weeks into 454 refined cell types and maps 114 spatial niches across 6PCW whole-embryo sections.

Hormonally responsive bovine oviductal organoids recapitulate native oviductal secretions and enhance sperm capacitation

Bovine oviductal organoids treated with estradiol or a progesterone analog reproduce follicular and luteal transcriptomes and secretomes; estradiol-stimulated secretions boost bull sperm capacitation and acrosome reaction.

A panoramic view of the expression and function of the Doublesex/DMRT gene family in C. elegans

Reporter alleles of all ten C. elegans DMRT genes show six are sexually dimorphic across tissues; mab-3, dmd-3 and mab-23 nulls alter male neuronal identities.

A spatial transcriptomics atlas of live donors reveals unique zonation patterns in the healthy human liver

Spatial transcriptomics of live healthy donor livers shows most hepatocyte genes are zonated, with key functions pericentrally shifted versus mouse, pig, boar and cow.

Peaceful Queen Succession in the Naked Mole Rat

Relocating a captive naked mole rat colony paused the queen's breeding; two daughters then sequentially became breeders without aggression, ending in peaceful succession.

Oncogenic and tumor-suppressive forces converge on a progenitor niche at the benign-to-malignant transition

In mouse pancreatic cancer models, p53, CDKN2A and SMAD4 programs converge on a rare Kras-mutant progenitor-like state whose niche KRAS inhibition collapses and p53 loss expands.

The Portal Project: a long-term study of a Chihuahuan desert ecosystem

The Portal Project archives over 40 years of continuous monthly rodent, biannual plant, multi-decade ant, and automated weather data from 24 experimentally manipulated Chihuahuan Desert plots with automated quality control.

{micro}CT Scanning Effects on DNA and a Multi-Step Workflow for Archaeological Petrous Bones

Fifty micro-CT-scanned archaeological petrous bones from Argentina did not differ significantly from 43 unscanned ones on six ancient DNA metrics, motivating a multi-step workflow that scans bones before destructive sampling.

PARAS: high-accuracy machine-learning of substrate specificities in nonribosomal peptide synthetases

Random forest predictors PARAS and PARASECT, trained on 3,653 curated adenylation domains, outperform earlier NRPS substrate predictors and correctly assigned L-Trp to an unusual tryptopeptin synthetase domain.