H. Gong

Human Amniotic Epithelial Cells Promote Chx10-/Pax6+ Muller Glia Subpopulation Reprogramming into Photoreceptor-like Cells

This study identifies a specific Muller glia subpopulation that can be reprogrammed into photoreceptor-like cells through transplantation of human amniotic epithelial cells.

De novo multi-mechanism antimicrobial peptide design via multimodal deep learning

This paper introduces M3-CAD, a deep learning pipeline for designing multi-mechanism antimicrobial peptides to combat multidrug-resistant organisms, showing effectiveness in a skin wound infection model.

Macrophage mediated mesoscale brain mechanical homeostasis mechanically imaged via optical tweezers and Brillouin microscopy in vivo

The paper uses optical tweezers and Brillouin microscopy to study brain mechanical properties in larval zebrafish, showing macrophages influence tissue mechanics during development.

TopoDiff: Improving Protein Backbone Generationwith Topology-aware Latent Encoding

TopoDiff improves protein backbone generation by using a topology-aware latent encoding to enhance controllability and coverage of generated structures.

YAP localization mediates mechanical adaptation of human cancer cells during extravasation in vivo

This study investigates how YAP-mediated mechanogenotypes influence mechanical adaptation of cancer cells during extravasation and organ colonization in vivo.

The structural OFF and ON states of myosin can be decoupled from the biochemical super-relaxed and disordered-relaxed states.

This study decouples structural OFF/ON states from biochemical SRX/DRX states in myosin using X-ray diffraction and ATP assays in porcine myocardium.

Myosin in autoinhibited off state(s), stabilized by mavacamten, can be recruited via inotropic effectors

This paper shows that mavacamten stabilizes myosin in off states, which can still be recruited by physiological inotropic effectors to maintain cardiac function.

Widespread PRC barrel proteins play critical roles in archaeal cell division

This study identifies three PRC barrel proteins in Haloferax volcanii essential for archaeal cell division, showing their conservation and role in division across haloarchaea.

Right Ventricular Sarcomere Contractile Depression and the Role of Thick Filament Activation in Human Heart Failure with Pulmonary Hypertension

This study identifies contractile deficits in right ventricular myocytes of HFrEF-PH patients, showing reduced calcium-activated tension and altered myosin ATP turnover, with implications for targeted therapies.

Development of an affinity-enhanced clinical candidate TCR targeting NY-ESO-1 with optimal potency and high specificity

This study develops a high-affinity, specific TCR targeting NY-ESO-1 through engineering and screening, showing efficacy in tumor models and approval for clinical trials.

Origins of monkeypox viruses and their strains circulating in 2022

This paper uses phylogenetic analysis to trace the origins of monkeypox viruses and their 2022 circulating strains, identifying possible origins from cowpox viruses and two lineages linked to 2021 exported strains.

Thick Filament Activation Is Different in Fast and Slow-Twitch Skeletal Muscle

This study uses X-ray diffraction to compare thick filament activation dynamics in fast and slow-twitch rat muscles, finding distinct structural transitions that may explain their differing contraction properties.

Insulin sensitivity is preserved in mice made obese by feeding a high starch diet

This study shows that mice fed a high starch diet become obese but remain insulin sensitive, unlike those on a high fat diet, with differences in ceramide levels and lipogenesis contributing to this effect.

Integrated optical device for Structured Illumination Microscopy

The paper presents an integrated optical chip for structured illumination microscopy, enabling high-resolution imaging by generating a hexagonal illumination pattern without alignment.

Isometric Spiracular Scaling in Scarab Beetles: Implications for Diffusive and Advective Oxygen Transport

This study examines the scaling of spiracles in scarab beetles, finding that diffusive capacities scale isometrically but require higher oxygen gradients in larger species, while advective capacities match respiratory demands during activity.

Cardiac myosin filaments are directly regulated by calcium

This study shows that cardiac myosin filaments are directly regulated by calcium, transitioning from inactive to active states through structural changes.

Myofibril orientation as a metric for characterizing heart disease

This paper introduces a method to quantify myofibrillar orientation in cardiac muscle and shows it detects greater disorientation in diseased tissue compared to healthy controls.

Regional Cell Atlas of Human Intestine Shapes Distinct Immune Surveillance

The paper creates a regional cell atlas of human intestine using single-cell RNA sequencing and spatial transcriptomics, revealing how microenvironment shapes immune surveillance and disease susceptibility.

Surface Engineering of FLT4-Targeted Nanocarriers Enhances Cell-Softening Glaucoma Therapy

This paper develops FLT4-targeted nanocarriers for glaucoma therapy, showing improved IOP reduction and targeted delivery to Schlemms canal cells.

Structure of mycobacterial cytochrome bcc in complex with Q203 and TB47, two anti-TB drug candidates

The paper reports the structures of M. tuberculosis cytochrome bcc complexed with two anti-TB drugs, revealing binding sites and interactions that inform inhibitor design.

A spatial and cellular distribution of neurotropic virus infection in the mouse brain revealed by fMOST and single cell RNA-seq

This study uses fMOST and scRNA-seq to map the spatial and cellular distribution of rabies virus in the mouse brain, revealing infection in multiple nuclei and non-neuronal cells.

Genetic analysis of the septal peptidoglycan synthase FtsWI complex supports a conserved activation mechanism for SEDS-bPBP complexes

The paper identifies mutations in FtsW and FtsI that reveal the activation mechanism of the SEDS-bPBP complex FtsWI, showing it relies on an interaction between FtsW's ECL4 and FtsI's pedestal domain.

The Ca2+-activated cation channel TRPM4 is a positive regulator of pressure overload-induced cardiac hypertrophy

This study identifies TRPM4 as a key regulator of pressure overload-induced cardiac hypertrophy by modulating CaMKII{delta}-HDAC4-MEF2A and calcineurin-NFAT-GATA4 pathways in mice.

Oxidized LDL but not angiotensin II induces the interaction between LOX-1 and AT1 receptors

This study shows Ox-LDL, not AngII, induces LOX-1 and AT1-R interaction, leading to cardiomyocyte hypertrophy via Gq protein activation.

The therapeutic effects of intratumoral injection of IL12IL2GMCSF fusion protein on canine tumors

This study evaluates the therapeutic effects of IL12IL2GMCSF fusion protein in canine tumors, showing significant response rates with minimal adverse effects.

Dietary supplementation with products of Citrus reticulata "Chachi" for improving the fecal microbiome of weaned piglets

This study examines the effects of dietary Citrus reticulata 'Chachi' products on the fecal microbiome of weaned piglets using multi-omics analyses, showing changes in microbial diversity, SCFAs, and proteomic profiles.

The insert sequence in SARS-CoV-2 enhances spike protein cleavage by TMPRSS

This study identifies an insertion sequence in SARS-CoV-2 spike protein that enhances cleavage efficiency and expands tissue tropism beyond the lungs.

The digestive system is a potential route of 2019-nCov infection: a bioinformatics analysis based on single-cell transcriptomes

This study identifies ACE2-expressing cells in the digestive system using single-cell transcriptomes, suggesting the digestive tract as a potential route for 2019-nCov infection.

MACS: Rapid aqueous clearing system for three-dimensional mapping of intact organs

MACS is a rapid aqueous clearing method that enables 3D imaging of intact organs with high transparency and compatibility with lipophilic dyes.

TLR3 Deficiency Leads to Altered Immune Responses to Chlamydia trachomatis Infection in Human Oviduct Epithelial Cells

This study shows that TLR3 deficiency in human oviduct epithelial cells alters immune responses to Chlamydia trachomatis infection, increasing inflammation and pathogenesis markers.