M. F. Krummel

Critical role of CD206+ macrophages in promoting a cDC1-NK-CD8 T cell anti-tumor immune axis

This study shows that CD206+ macrophages support an anti-tumor network involving cDC1, NK cells, and CD8 T cells, and their depletion impairs tumor control in mice and correlates with poor survival in humans.

Multimodal delineation of a layer of effector function among exhausted CD8 T cells in tumors

The paper identifies a potent effector state among exhausted CD8 T cells in tumors using multimodal CITE-Seq, showing enhanced cytokine production and tumor-killing ability.

Tumor cell heterogeneity drives spatial organization of the intratumoral immune response in squamous cell skin carcinoma

This study shows that tumor cell heterogeneity influences the spatial organization of immune responses in squamous cell skin carcinoma, affecting immunotherapy outcomes.

Cyclone: an accessible pipeline to analyze, evaluate and optimize multiparametric cytometry data

Cyclone is a pipeline for analyzing, evaluating, and optimizing multiparametric cytometry data, demonstrating effectiveness across multiple cytometry technologies and biological contexts.

Uptake of tumor-derived microparticles induces metabolic reprogramming of macrophages in the early metastatic lung.

This study shows that uptake of tumor-derived microparticles by macrophages in the early metastatic lung leads to metabolic reprogramming and altered function.

Dynamic CD8+ T cell responses to cancer immunotherapy in human regional lymph nodes are disrupted by metastasis

The paper examines CD8+ T cell responses in human lymph nodes during cancer immunotherapy and finds that metastasis disrupts these responses.

Immediate myeloid depot for SARS-CoV-2 in the human lung

The paper investigates early SARS-CoV-2 infection in human lung myeloid cells, finding rapid viral tropism, ACE2 dependence, and poor interferon induction, suggesting a myeloid depot for viral persistence.

A conserved immune trajectory of recovery in hospitalized COVID-19 patients

The paper identifies a conserved immune recovery trajectory in hospitalized COVID-19 patients through longitudinal mass cytometry analysis, linking immune processes to clinical outcomes.

T cells use distinct topological and membrane receptor scanning strategies that individually coalesce during receptor recognition

This study shows T cells use independent scanning strategies for microvilli and receptor patches, which coalesce during receptor recognition.

Impact of Protein Identity on Tumor-Associated Antigen Uptake into Infiltrating Immune Cells: A Comparison of Different Fluorescent Proteins as Model Antigens

This study compares six fluorescent proteins as model antigens to assess their uptake and distribution in tumor-infiltrating immune cells and draining lymph nodes, finding variability in detection and transport based on fluorophore properties.

Spatiotemporal co-dependency between macrophages and exhausted CD8+ T cells in cancer

This study investigates the interaction between macrophages and exhausted CD8+ T cells in the tumor microenvironment, showing they form a feedback loop that promotes tumor survival.

Visualizing and manipulating hyperstabilization of T cell microvilli contacts by engineered chimeric antigen receptors

This study uses advanced imaging to show that engineered CARs with high affinity hyperstabilize T cell microvilli contacts, impairing function, while reducing affinity restores normal dynamics and improves effector function.

Holistic Characterization of Tumor Monocyte-to-Macrophage Differentiation Integrates Distinct Immune Phenotypes in Kidney Cancer

This study characterizes monocyte-to-macrophage differentiation in kidney cancer using scRNA-seq, linking immune phenotypes to T cell function and patient outcomes.

Multiomic Characterization of Stage I Lung Adenocarcinoma Reveals Distinct Genetic and Immunologic Features of Recurrent Disease

This study identifies distinct genetic and immunologic features of recurrent stage I lung adenocarcinoma using multiomic profiling and single-cell RNA-seq.

Active Surveillance Characterizes Human Intratumoral T Cell Exhaustion

The paper studies T cell exhaustion in human tumors using live-biopsy and two-photon microscopy, finding variable T cell speed and a link between motility and exhaustion state.

A Pan-Cancer Census of Dominant Tumor Immune Archetypes

The paper identifies dominant immune system archetypes across 364 tumors from 12 cancer types using flow cytometry and transcriptomic data, linking them to tumor biology classifications.

Longitudinal single-cell epitope and RNA-sequencing reveals the immunological impact of type 1 interferon autoantibodies in critical COVID-19

The paper examines IFN-I autoantibodies in critical COVID-19 patients through longitudinal single-cell analysis, revealing suppressed ISG-I responses in myeloid cells.

Activating Immune Recognition in Pancreatic Ductal Adenocarcinoma Using Autophagy Inhibition, MEK blockade and CD40 Agonism

This study shows that combining MEK inhibition, autophagy inhibition, and CD40 agonism activates the Type I Interferon/STING pathway in PDA tumors, reprograms TAMs, and enhances anti-tumor immunity in a mouse model.

Global Absence and Targeting of Protective Immune States in Severe COVID-19.

The paper identifies that severe COVID-19 patients lack ISG-expressing cells and have antibodies that block their production, contrasting with mild cases.

Learned adaptive multiphoton illumination microscopy

This paper develops a machine learning model to adaptively adjust multiphoton microscopy illumination power based on in situ cell imaging, enabling improved in vivo immune response visualization in mouse lymph nodes.

SCENITH: A flow cytometry based method to functionally profile energy metabolism with single cell resolution

SCENITH functionally profiles energy metabolism at single-cell resolution using flow cytometry, revealing variable metabolic profiles in tumor myeloid cells not linked to lineage or activation phenotype.

WAVE complex self-organization templates lamellipodial formation

The paper investigates how the WAVE complex self-organizes into linear structures to form lamellipodia, contrasting with N-WASP's spiky actin networks, and shows how curvature sensing enables cell shape regulation.

Tumors Exploit Dedicated Intracellular Vesicles to Program T cell Responses

The paper shows myeloid cells transfer tumor antigen-laden vesicles to APCs, directing T cell responses away from memory phenotypes.

Pulmonary natural killer cells control neutrophil intravascular motility and response to acute inflammation

This study shows pulmonary natural killer cells reduce neutrophil accumulation during inflammation by altering their motility.