J. Chen

Inflammasome targeting with an NLRP3 agonist therapy is feasible but ineffective in murine hepatocellular carcinoma models with liver damage

This study tests an NLRP3 agonist added to PD-1/VEGFR inhibitors in murine HCC models with liver damage, finding it feasible but ineffective in improving tumor outcomes.

The Optimal Combination of Scanning Speed and Fiber-to-Stone Distance for Effective and Efficient Dusting during Holmium: YAG Laser Lithotripsy

This study identifies the optimal scanning speed and fiber-to-stone distance for maximizing dusting efficiency during Holmium: YAG laser lithotripsy by comparing photothermal ablation and cavitation effects.

The cohesin modifier ESCO2 is stable during DNA replication

This study shows ESCO2 remains stable during DNA replication in Xenopus egg extracts and transgenic somatic cells, even under DNA damage or increased replication conditions.

Combined transcriptome and metabolome analysis of a new species of microalgae from Tibetan plateau and its response to sewage treatment

This study combines transcriptome and metabolome analysis to identify molecular responses of Desmodesmus sp. to sewage treatment, revealing key pathways and metabolite changes.

Sex-specific niche signaling contributes to sexual dimorphism following stem cell transplantation

This paper identifies sex-specific niche signaling differences in hematopoietic regeneration following stem cell transplantation and shows exogenous sex hormones regulate hematopoietic stem and progenitor cells.

Contrasting autoimmune and treatment effects reveals baseline set points of immune toxicity following checkpoint inhibitor treatment

The paper identifies pre-treatment immune signatures associated with immune-related adverse events (irAEs) in thymic cancer patients receiving checkpoint inhibitors using CITE-seq.

Pathogen-Host Adhesion between SARS-CoV-2 S Proteins from Different Variants and Human ACE2 Probed at Single-Molecule and Single-Cell Levels

This study investigates the adhesion of SARS-CoV-2 S proteins from different variants to human ACE2 using single-molecule and single-cell force spectroscopy, finding Delta variant has the highest binding affinity.

Structural basis for substrate selection by the SARS-CoV-2 replicase

This study uses cryo-EM to reveal how the SARS-CoV-2 RTC discriminates between natural NTPs and the antiviral remdesivir triphosphate, providing insights for antiviral design.

An atlas of epithelial cell states and plasticity in lung adenocarcinoma

This study maps epithelial cell states and plasticity in lung adenocarcinoma using single-cell data, identifying KRT8+ alveolar intermediate cells linked to poor survival and tumorigenesis.

Multi-color live-cell STED nanoscopy of mitochondria with a gentle inner membrane stain

This paper introduces a new fluorescent marker, PKMO, enabling 3D live-cell STED nanoscopy of mitochondria with improved photostability and reduced phototoxicity, allowing multiplexed imaging of mitochondrial dynamics and interactions.

Dynamic HIV-1 spike motion creates vulnerability for its membrane-bound tripod to antibody attack

The paper investigates HIV-1 spike protein dynamics using cryo-EM and simulations, showing how tilting exposes the MPER to antibody attack and alters membrane fusion.

Mapping Strigolactone Hydrolysis in DWARF14 via QM/MM String Method

This study uses QM/MM simulations to investigate the mechanism of strigolactone hydrolysis by DWARF14, finding the acyl substitution pathway is favored and covalent modifications are dynamic.

The senescence regulator S40 family members from Caragana intermedia and Arabidopsis thaliana inhibit leaf senescence via promoting cytokinins synthesis

This study identifies CiS40-11 and its orthologs AtS40-5 and AtS40-6 as regulators of leaf senescence that promote cytokinin synthesis by inhibiting MYB2 expression.

Climate and competition combine to set elevational distributions of tropical rainforest Drosophila

This study examines how climate and competition influence elevational distributions of tropical Drosophila species in the Australian Wet Tropics, finding that thermal tolerance and interspecific competition drive community composition at different elevations.

The Structural and Biochemical Basis for FER Receptor Kinase Early Signaling Initiation in Arabidopsis

This study investigates the structural and biochemical basis of early FER receptor kinase signaling in Arabidopsis, revealing an active conformation and the role of Tyr phosphorylation in substrate activation.

PPAR-γ activation promotes xenogenic bioroot regeneration by attenuating the xenograft induced-oxidative stress

This study shows that PPAR-γ activation with rosiglitazone reduces oxidative stress and promotes xenograft regeneration in a dental follicle cell model.

Dual-Resolving of Lipid Positional and Geometric Isomers of C=C Bonds via Bifunctional Photocycloaddition-Photoisomerization Reaction System and Mass Spectrometry

This paper develops a bifunctional reaction system to simultaneously resolve lipid positional and geometric isomers using mass spectrometry, revealing structural patterns in bacteria and tracking changes in mouse brain ischemia.

Genetic engineering of Acidithiobacillus ferridurans with CRISPR-Cas9/dCas9 systems

This paper develops two CRISPR-based systems for genetic manipulation in Acidithiobacillus ferridurans, achieving efficient gene knockdown and genome editing with significant effects on sulfur and iron metabolism.

De-heterogeneity of the eukaryotic viral reference database (EVRD) improves the accuracy and efficiency of viromic analysis

The paper describes the purification of a viral reference database (EVRD) by removing contaminating sequences, improving accuracy and efficiency in viromic analysis.

Balancing activation and costimulation of CAR tunes signaling dynamics and enhances therapeutic potency

This study shows that modifying CAR activation motifs improves anti-tumor efficacy in solid tumors despite reduced in vitro cytotoxicity.

Loss of E-cadherin Induces IGF1R Activation Revealing a Targetable Pathway in Invasive Lobular Breast Carcinoma

This study shows that loss of E-cadherin in ILC leads to increased IGF1R activation and sensitivity to inhibitors, suggesting a therapeutic target and biomarker for ILC.

Activation Mechanism of Strigolactone Receptors And Its Impact On Ligand Selectivity Between Host And Parasitic Plants

This study uses molecular dynamics simulations to show that ShHTL7 and AtD14 adopt active conformations without ligand, but ShHTL7 has a higher population in the inactive state due to sequence differences affecting binding pocket closure.

Lyophilized mRNA-lipid nanoparticle vaccines with long-term stability and high antigenicity against SARS-CoV-2

This paper develops lyophilized mRNA-lipid nanoparticle vaccines that maintain stability at room temperature and show high antigenicity against SARS-CoV-2 variants in preclinical and human trials.

Plasticity in structure and assembly of SARS-CoV-2 nucleocapsid protein

The paper analyzes sequence and structural variability in the SARS-CoV-2 nucleocapsid protein, finding high mutability and structural plasticity linked to enhanced viral assembly efficiency.

Superparamagnetic iron oxide enclosed hollow gold nanostructure with tunable surface plasmon resonances to promote near-infrared photothermal conversion

This paper designs and synthesizes SPIO-HGNS nanoparticles with tunable SPR in the NIR range to enhance photothermal conversion for potential photothermal therapies.

Structural features of mTORC2 that control substrate-specific activities.

The paper determines the structure of mTORC2 and shows how substrate-specific interactions and conformational changes regulate its activity.

PDX models of relapsed pediatric AML preserve global gene expression patterns and reveal therapeutic targets

This study shows that PDX models of pediatric AML retain patient-like gene expression patterns and identify therapeutic targets.

An allosteric agonist activates BK channels by perturbing coupling between Ca2+ binding and pore opening

The paper identifies BC5, an allosteric agonist that modulates BK channel activation by disrupting the coupling between Ca2+ binding and pore opening.

Heterotrimeric G protein α subunit (GPA1) regulates the response to low-nitrogen stress in Arabidopsis by interacting with AtNRT1.4 and AtATG8a

AtGPA1 regulates low-nitrogen stress response in Arabidopsis by interacting with AtNRT1.4 and AtATG8a, affecting nitrate uptake and autophagy.

Single-Cell Transcriptomic Analyses of Tumor Ecosystems and Spatial Architectures in Human Small Cell Lung Cancers

This study uses single-cell and spatial transcriptomics to analyze heterogeneity and immunosuppressive microenvironment in human SCLC, identifying hybrid tumor cell states and their origins.