K. Chandran

Distinct pathway for evolution of enhanced receptor binding and cell entry in SARS-like bat coronaviruses

The paper investigates how SARS-like bat coronaviruses evolve enhanced receptor binding and cell entry through specific genetic substitutions.

A viral vaccine design harnessing prior BCG immunization confers protection against Ebola virus

This paper demonstrates a viral vaccine strategy using BCG-induced Th cells to enhance antibody responses against Ebola virus, providing protection in a mouse model.

Antibodies targeting Crimean-Congo hemorrhagic fever virus GP38 limit vascular leak and viral spread

This study identifies GP38 as a viral toxin causing vascular leak and shows that specific GP38-targeting antibodies block this effect in vitro and in vivo.

Engineering, structure, and immunogenicity of a Crimean-Congo hemorrhagic fever virus pre-fusion heterotrimeric glycoprotein complex

The paper describes the structure and immunogenicity of a stabilized CCHFV heterotrimeric glycoprotein complex and shows it provides 40% protection against viral challenge in mice.

Crimean-Congo Hemorrhagic Fever Survivors Elicit Protective Non-Neutralizing Antibodies that Target 11 Overlapping Regions on Viral Glycoprotein GP38

The paper identifies non-neutralizing antibodies from CCHFV survivors that target multiple regions on GP38, showing protective efficacy in rodent models.

Simulation-Driven Design of Stabilized SARS-CoV-2 Spike S2 Immunogens

This study designs stabilized SARS-CoV-2 S2 immunogens using simulation-driven approaches, showing improved stability and immunogenicity.

Fine-tuning spatial-temporal dynamics and surface receptor expression support plasma cell-intrinsic longevity

This study identifies unique features of long-lived plasma cells (LLPCs), including reduced motility, clustering, and specific surface markers, and shows that CXCR4 is critical for their survival and antibody production.

Divergent microbial structure still results in convergent microbial function during arrested anaerobic digestion of food waste at different hydraulic retention times

This study shows that different microbial structures in anaerobic digesters with varying hydraulic retention times result in similar VFA production due to functional redundancy.

Two point mutations in protocadherin-1 disrupt Andes hantavirus recognition and afford protection against lethal infection

The paper identifies two point mutations in PCDH1 that disrupt ANDV recognition and protect against lethal infection in Syrian hamsters.

Reovirus infection is regulated by NPC1 and endosomal cholesterol homeostasis

This study identifies NPC1 and endosomal cholesterol homeostasis as critical for reovirus penetration into the cytoplasm, but not for attachment, internalization, or disassembly.

Glycerol-driven Denitratation: Process Kinetics, Microbial Ecology, and Operational Controls

This study optimizes glycerol-driven denitratation for nitrogen removal, showing higher nitrate reduction rates and identifying operational controls and microbial ecology factors.

Genotype-specific Features Reduce the Susceptibility of South American Yellow Fever Virus Strains to Vaccine-Induced Antibodies

This study shows that South American yellow fever virus strains are less susceptible to vaccine-induced antibodies due to genetic changes in the glycoprotein E.

A combination of RBD and NTD neutralizing antibodies limits the generation of SARS-CoV-2 spike neutralization-escape mutants

This paper identifies NTD-targeting neutralizing antibodies and shows that combining RBD and NTD antibodies reduces the emergence of neutralization-escape mutants.

A glycoprotein mutation that emerged during the 2013-2016 Ebola virus epidemic alters proteolysis and accelerates membrane fusion

This paper investigates how the A82V mutation in the Ebola virus glycoprotein enhances infectivity by altering proteolysis and accelerating membrane fusion.

MAVERICC: Efficient marker-free rescue of vaccinia virus recombinants by in vitro CRISPR-Cas9 engineering

MAVERICC enables marker-free, efficient engineering of vaccinia virus recombinants using in vitro CRISPR-Cas9 cleavage, producing pure recombinants in a single step.

Genetic depletion studies define receptor usage by virulent hantaviruses in human endothelial cells

This study uses CRISPR/Cas9 to knock out potential hantavirus receptors in human endothelial cells, finding that PCDH1 is critical for some hantaviruses while other receptors like integrins and DAF are not essential.

The evolutionary history of ACE2 usage within the coronavirus subgenus Sarbecovirus

This study investigates the evolutionary history of ACE2 usage in sarbecoviruses, showing that ACE2 usage in SARS-CoV-1 and SARS-CoV-2 likely arose through recombination with SARS-CoV-1 relatives, not SARS-CoV-2.

Characterization of the SARS-CoV-2 S Protein: Biophysical, Biochemical, Structural, and Antigenic Analysis

This study evaluates the production, characterization, and antigenicity of SARS-CoV-2 S protein constructs in two cell lines, showing high-quality yields and consistent behavior across assays.

A replication-competent vesicular stomatitis virus for studies of SARS-CoV-2 spike-mediated cell entry and its inhibition

This paper develops a recombinant vesicular stomatitis virus expressing SARS-CoV-2 spike protein to assess neutralizing antibodies in convalescent sera and validate its utility for vaccine and therapeutic development.

Broad sarbecovirus neutralizing antibodies define a key site of vulnerability on the SARS-CoV-2 spike protein

The paper identifies broadly neutralizing antibodies against SARS-CoV-2 and related sarbecoviruses, highlighting a conserved epitope on the spike protein as a potential vaccine target.

Direct intracellular visualization of Ebola virus-receptor interaction by in situ proximity ligation

This paper introduces an in situ proximity ligation assay to visualize Ebola virus glycoprotein (GPCL) interaction with the receptor NPC1 in infected cells, revealing insights into viral entry mechanisms and drug inhibition.

Phenotypic heterogeneity in particle size is a viral mechanism of persistence

This study shows that filamentous viral particles have a functional advantage over spherical ones in resisting neutralizing antibodies and fusion inhibitors.

A virion-based assay for glycoprotein thermostability reveals key determinants of filovirus entry and its inhibition

This paper introduces an ELISA-based assay to measure the thermostability of filovirus glycoproteins, revealing how proteolytic cleavage, acid pH, and small-molecule inhibitors affect GP stability and viral entry.

Accelerated viral dynamics in bat cell lines, with implications for zoonotic emergence

The paper models viral dynamics in bat cell lines with constitutive immune responses, finding that robust antiviral defenses correlate with higher viral propagation rates and suggest implications for zoonotic emergence.

The generation of plasma cells and CD27-IgD- B cells during Hantavirus infection are associated with distinct pathological findings

This study links plasma cell and CD27-IgD- B cell generation during Hantavirus infection to distinct pathological features in HFRS.

Human, Nonhuman Primate, and Bat Cells Are Broadly Susceptible to Tibrovirus Particle Cell Entry

The paper shows that tibrovirus glycoproteins can enter human, bat, and nonhuman primate cells, but replication resistance varies among cell types.

Human antibody cocktail deploys multiple functions to confer pan-ebolavirus protection

This paper develops a human antibody cocktail, MBP134, that provides broad protection against multiple ebolaviruses, showing enhanced efficacy in guinea pigs compared to individual antibodies.

Two-antibody pan-ebolavirus cocktail confers broad therapeutic protection in ferrets and nonhuman primates

This paper demonstrates that a two-antibody cocktail (MBP134AF) provides broad therapeutic protection against multiple ebolaviruses in ferrets and nonhuman primates.

Structural Basis of Broad Ebolavirus Neutralization by a Human Survivor Antibody

The paper determines the structure of a broadly neutralizing antibody against ebolavirus, identifies a conserved epitope, and explains factors affecting its activity against related viruses.

Two point mutations in the Hantaan virus glycoproteins afford the generation of a highly infectious recombinant vesicular stomatitis virus vector

This paper identifies two point mutations in Hantaan virus glycoproteins that enhance their incorporation into recombinant VSV particles, enabling the generation of a highly infectious rVSV vector for hantavirus research.