CRISPR screen

This study reprograms genetic circuits in bacteria by spatially positioning circuit components to optimize performance and enable new functions without altering DNA sequences.

This paper describes a CRISPR-dCas13 system to induce stop codon readthrough in specific transcripts, showing enhanced readthrough in AGO1, VEGFA, and HBB mRNAs.

CROPseq-multi enables multiplexed CRISPR perturbations across various screening methods with improved barcode detection and compatibility.

This study uses CRISPR-A to enhance expression of CHD8 and SCN2A in organoids, rescuing haploinsufficiency and associated phenotypes in ASD models.

This paper introduces a trisomy 21-linked CSF2RB mutation in microglia that reduces neuroinflammation and enhances phagocytic functions, offering potential for AD therapy.

The paper develops a Sparse supervised autoencoder to detect subtle transcriptomic changes from CRISPRi screens of lncRNAs in lung cancer cells under hypoxia.

The paper introduces a modular CRISPR vector assembly system called Fragmid, enabling rapid creation and testing of CRISPR technologies through combinatorial design and pooled screening.

The paper develops CRISPRtOE, a genome-scale overexpression platform for Proteobacteria, demonstrating its use in Escherichia coli and Zymomonas mobilis to identify antibiotic and engineering targets.

The paper develops a machine learning model to identify functional lncRNAs and validates SNHG6's role in hematopoietic differentiation and leukemia.

The paper develops a CRISPRi-based screening platform to identify genes affecting neuronal excitability in human iPSC-derived neurons, revealing new links to neurodegenerative and neurodevelopmental disorders.

This paper introduces CRISTAL, a platform for programmable RNA regulation using inducible Cas13 circuits, enabling precise temporal control and multiplexed gene regulation in mammalian cells.

The paper demonstrates that CRISPR-Cas9 nickase can contract CAG/CTG repeats in patient-derived cells and improve molecular and behavioral phenotypes in Huntington's disease.

The paper develops a high-activity CRISPR-Cas9 library for Komagataella phaffii and uses it to identify essential genes related to protein production, secretion, and glycosylation.

This paper develops a method to efficiently reprogram T cells at multiple loci using SEED-Selection, achieving high purity for single and combined gene edits.

This study repurposes CRISPR-Cas13 systems to enable trans-splicing of exogenous RNA fragments into endogenous pre-mRNA, improving editing of large mRNA segments in mammalian cells.

This paper demonstrates efficient in vivo CRISPR gene editing using a compact Cas12f system delivered via AAV, showing safety and targeting multiple cell types.

This study develops personalized CRISPR-Cas9 strategies to specifically inactivate the mutant BAG3 allele in MFM6 patients, demonstrating their feasibility for regenerative medicine.

This study identifies TET2 and INO80 as key genes in kidney disease pathogenesis through GWAS, single-cell analysis, and mouse models, showing their role in DNA damage response and disease progression.

The paper demonstrates that chemically modified ssDNA (eDNA) significantly improves gene insertion efficiency in primary cells compared to unmodified ssDNA, achieving therapeutic levels in clinically relevant loci.

This study investigates how RNA structure affects Cas13 activity, revealing two mechanisms by which secondary structure modulates Cas13 and improving RNA detection through strand displacement.

This paper introduces NSC-seq, a direct sgRNA capture method for single-cell CRISPR screens using standard knockout libraries, and characterizes sgRNA expression in three whole-genome libraries.

This paper introduces Crackling++, a faster and scalable method for off-target assessment of CRISPR guide RNAs by using non-contiguous exact matches, outperforming existing tools.

Chromatin regulators including EZH2, EHMT1/2, and DOT1L establish an epigenetic barrier in human neural progenitors that poises maturation genes and sets the protracted pace of cortical neuron maturation.

The paper presents a scalable CRISPR-Cas9 platform for chemical-genetic screens of DNA damage-inducing compounds, identifying enriched modes-of-action and revealing late ICL repair pathway responses.

This study shows BCOR is essential for syncytiotrophoblast differentiation in mice and humans by repressing stem cell genes and alternate lineage factors.

This study improves CRISPR editing in Daphnia pulex, reveals mutation mosaicism, and identifies gene expression changes linked to neurodegenerative disease genes in scarlet mutants.

The paper introduces graded-CRISPRi, a tool for tuning CRISPRi-mediated knockdown strengths in Vibrio natriegens using gRNA libraries to study gene expression effects.

This study develops hyperactive base editors by exploring Cas9 and AID cooperation, revealing mechanisms that expand mutational diversity and efficiency in base editing.

This paper demonstrates the delivery of functional Cas:DNA nucleoprotein complexes into recipient bacteria via a Type IV Secretion System, enabling targeted genetic modification without requiring nuclease expression in target cells.