Antifungal resistance and susceptibility

SQ109 is a Novel Multitarget Antifungal in Pathogenic Yeasts: Membrane-Active Δ8,14 Sterol Remodeling, Bioenergetic Stress, and Vacuolar Dysfunction

This paper shows SQ109 targets multiple pathways in pathogenic yeasts, causing sterol accumulation, proton gradient collapse, and vacuolar dysfunction, leading to antifungal synergy and low resistance.

Nationwide Spread of Fluconazole-Resistant Candida parapsilosis Clones: Insights from the Antifungal Resistance Surveillance Program

The paper tracks the spread of fluconazole-resistant Candida parapsilosis clones in Spain, identifying multiple resistance mechanisms and clonal lineages across 31 hospitals.

CRISPR-activation reveals key resistance genes and vulnerabilities of copy number variants in Candida albicans

This study uses CRISPR-activation to identify genes with fitness effects in Candida albicans isolates, revealing resistance genes and vulnerabilities associated with copy number variants.

Repurposing the Antiviral Agent Pibrentasvir: In Vitro Synergistic Effects in Combination with Different Azole Antifungal Agents

This study evaluates the in vitro synergistic effects of pibrentasvir combined with azole antifungal agents against several fungal pathogens.

Systematic Screening of a Glycolysis-targeting Small-molecule Library Identifies Novel Inhibitors of Fungal Morphogenesis in Candida albicans

This study identifies two novel glycolysis inhibitors that block fungal morphogenesis in C. albicans by targeting pyruvate kinase, enhancing antifungal susceptibility and reducing virulence.

The Aspergillus fumigatus C2-Domain Protein SppA is required for septal integrity and alters susceptibility to echinocandins and neutrophil killing during infection

This paper identifies SppA as a critical protein for septal integrity in A. fumigatus, showing its role in antifungal tolerance and virulence.

Opportunistic pathogenicity in fungi can transcend species boundaries

This study compares phenotypic, metabolomic, and genomic variation between two Aspergillus species to determine factors distinguishing opportunistic pathogens from non-pathogens.

The Unique Efg1 Fungal Virulence Regulon in the Catheterized Bladder Environment

This study identifies the Efg1 regulon active in C. albicans during growth in human urine, validates its clinical relevance in catheter samples, and characterizes key downstream factors involved in fungal CAUTI.

Broad remodeling of the pulmonary immune landscape occurs during IAPA with specific functional deficits of neutrophil subsets

This study identifies broad immune remodeling and neutrophil subset dysfunction in IAPA using single-cell transcriptomics and functional assays.

IL-27 Signaling Protects Against Influenza-Associated Pulmonary Aspergillosis Through Inhibition of Type 2 Immunity and Enhanced Antifungal Immunity

This study shows IL-27 signaling protects against influenza-associated pulmonary aspergillosis by reducing type 2 immunity and enhancing antifungal responses in mice.

Role of zinc in growth, stress response and virulence gene expression of pathogenic Mucorale Rhizopus arrhizus

This study shows zinc supplementation promotes growth and virulence gene expression in Rhizopus arrhizus, suggesting a link between zinc overuse and increased mucormycosis risk during the COVID-19 pandemic.

Candida glabrata YPK2 is a multidrug susceptibility locus

This study identifies Ypk2 as a multidrug susceptibility factor in C. glabrata by showing its role in modulating gene expression and drug resistance across antifungal classes.

M-CSF stimulated alveolar macrophages safeguard from invasive aspergillosis

This study shows that M-CSF enhances alveolar macrophage function to protect against invasive aspergillosis in immunocompromised mice.

Epistasis limits but does not prevent the transfer of mutation-drug resistance mapping across 600 million years of fungal evolution

The paper shows that resistance mutations to antifungal drugs are conserved across fungal species but epistasis limits their transferability.

Micafungin exposure drives multidrug resistance in Clavispora lusitaniae

This study shows that micafungin exposure selects for multidrug resistance in C. lusitaniae through mutations in ERG3, leading to sterol reprogramming and cell wall remodeling.

Short chain fatty acids potentiate azoles by reprogramming fungal acetyl-CoA metabolism

This study shows that short chain fatty acids (SCFAs) increase susceptibility of Candida albicans to fluconazole by reprogramming acetyl-CoA metabolism and reducing ergosterol biosynthesis.

HAC1 contributes to stress adaptation and virulence in the emerging fungal pathogen Candida auris

This study identifies HAC1 as a virulence-associated gene in Candida auris, showing its role in ER stress adaptation and host survival through unconventional splicing and functional analyses.

Antifungal resistance mechanisms and nosocomial transmission of Nakaseomyces glabratus: genomic investigation and observational study in Melbourne, Australia

This study identifies a nosocomial transmission cluster of fluconazole-resistant Nakaseomyces glabratus and novel resistance-associated variants in a Melbourne hospital.

Body temperature drives azole tolerance in Candida albicans by hindering the autophagic degradation of Erg11

This study shows that human body temperature increases azole tolerance in Candida albicans by reducing Erg11 degradation via autophagy inhibition.

Inferring antifungal drug synergy from Candidozyma auris optical density data using Bayesian mechanistic modelling

This paper develops a Bayesian mechanistic model to infer antifungal drug synergy and drug-action kinetics from optical density data of Candidozyma auris.

Skin commensal Micrococcus luteus Induces β-defensin-14 in keratinocytes and Enhances IL-17F Responses to Reduce Candida auris Colonization

This study shows that the skin bacterium Micrococcus luteus reduces Candida auris colonization by inducing β-defensin-14 and enhancing IL-17F responses.

A strain-specific metabolic role for the UDP-glucose 4-epimerase Uge3 in Aspergillus fumigatus virulence

The paper identifies a strain-specific metabolic role for Uge3 in H-MORPH A. fumigatus strains, linking it to virulence and central carbon metabolism.

Human metabolites modulate antifungal efficacy and reveal creatinine-mediated antagonism of flucytosine

The paper identifies human metabolites, particularly creatinine, that reduce the efficacy of the antifungal drug flucytosine by interfering with its uptake.

A long non-coding RNA regulates in vitro and in vivo triazole antifungal susceptibility in Aspergillus fumigatus

The paper identifies a long non-coding RNA, afu-182, that regulates azole antifungal susceptibility in Aspergillus fumigatus both in vitro and in vivo.

An epigenetic mechanism of azole tolerance facilitates acquired antifungal resistance in Aspergillus fumigatus

This study identifies IngB as an epigenetic regulator of azole tolerance in Aspergillus fumigatus, showing that its loss enables rapid acquired resistance under azole stress.

A targeted drug-repurposing strategy identifies Tavaborole (Kerydin) as a potent fungistatic agent against Candida auris

This study identifies Tavaborole as a potent fungistatic agent against Candida auris using a targeted drug-repurposing strategy and reveals resistance mechanisms through proteomics and electron microscopy.

Candidozyma auris utilizes transferrin, but not heme-bound iron for in vivo virulence

This study shows Candidozyma auris uses transferrin-bound iron for growth and virulence, not heme, and identifies transferrin saturation as a host susceptibility factor.

Antifungal tolerance and resistance dynamics depend on infection load, drug treatment, and host innate immunity

The paper models how antifungal tolerance and resistance in Candidozyma auris depend on infection load, drug treatment, and host immunity.

Establishing thresholds for azole tolerance and persistence in Aspergillus fumigatus to study their impact on voriconazole treatment in vivo

This study establishes thresholds for azole tolerance and persistence in Aspergillus fumigatus and shows tolerance negatively affects voriconazole treatment efficacy in a Galleria mellonella model.

Non-triazole agricultural fungicides indirectly select for triazole resistance in the human pathogen Aspergillus fumigatus

This study shows non-triazole fungicides can indirectly select for triazole resistance in Aspergillus fumigatus through genomic hitch-hiking.