Microtubule and mitosis dynamics

A role for tubulin in cellular quality control and proteostasis

This study shows that tubulin overexpression causes proteostasis defects and replication stress by disrupting mitochondrial translation and cell cycle regulation.

Loss of Tubulin Tyrosination in Purkinje Neurons Does Not Cause Their Degeneration

This study shows that loss of tubulin tyrosination in Purkinje neurons does not lead to their degeneration, unlike polyglutamylation dysregulation.

Tubulin autoregulation tunes microtubule dynamics to support multicellular architecture and viability

This study shows that TTC5-dependent tubulin autoregulation maintains microtubule dynamics, cytoskeletal organization, and tissue viability in human 3D models.

Spatiotemporal control of cortical centrin patterning by regionalized Sfi1 family scaffolding proteins in Stentor coeruleus

This study identifies Sfi1 family proteins as regulators of anterior-posterior centrin patterning in Stentor coeruleus, showing their role in regeneration and contraction through sequential recruitment during development.

A systems-level proteomic analysis identifies kinesin targets of KIFBP during neuronal development

This study identifies KIFBP-regulated kinesins during neuronal development using proteomic and imaging approaches, revealing KIF5A and KIF18B as novel targets.

DNA tensiometer reveals catch-bond detachment kinetics of kinesin-1, -2 and -3

This study measures detachment kinetics of kinesin-1, -2, and -3 under parallel forces using a DNA tensiometer, revealing load-dependent behavior and competition with dynein.

BubR1 TPR domain supports mitotic checkpoint by promoting MCC formation and MCC-APC/C interaction

This study identifies the BubR1 TPR domain's role in SAC signaling by promoting MCC formation and MCC-APC/C interaction through structural and functional analyses.

DNA catenation is essential for Sister Chromatid Cohesion

This study shows DNA catenation is essential for sister chromatid cohesion in budding yeast and metazoan chromosomes, as it provides force resistance necessary for accurate chromosome segregation.

Kinetochore clustering is mediated by Mps1 phosphorylation of conserved MELT motifs in Stu1

The paper shows Mps1 phosphorylates MELT motifs in Stu1 to recruit Slk19 and mediate kinetochore clustering in budding yeast.

ALMS1 contributes to centriole proximal architecture and stability

This study shows ALMS1 is essential for centriole proximal architecture and stability, with loss of ALMS1 causing structural defects in RPE1 cells.

ER shaping proteins guide spindle elongation and division during rapid cleavage mitoses

This study shows that Reep proteins regulate ER morphology during Drosophila cleavage mitoses, with ReepB playing a critical role in spindle elongation and division.

Feedback between filament spacing, crosslinker binding, and self-organization in cytoskeletal bundles

The paper develops a biophysical model showing how crosslinker binding and filament spacing feedback to self-organize cytoskeletal bundles into specific geometries.

Mutating the interprotofilament interface allows microtubules to assemble in GDP

The paper shows that microtubules can assemble in GDP by mutating the interprotofilament interface, suggesting that stable interprotofilament bonds, not GTP bias, drive dynamic instability.

Structural Basis of Kinesin-1 Autoinhibition and Its Control of Microtubule-Based Motility

This study determines the cryo-EM structure of autoinhibited kinesin-1 and identifies mechanisms of autoinhibition and activation.

Kinetochore-microtubule attachments are strengthened by Cnn1 stabilization of Stu2

This study shows that Cnn1 stabilizes Stu2 to strengthen kinetochore-microtubule attachments, improving attachment strength and cellular viability.

Dendritic varicosities revealed as important micro-tubule organisers in neurons

The paper identifies dendritic varicosities as a new type of MTOC in Drosophila neurons that organize minus-end-out microtubules.

Inhibition of microtubule polymerization and dynein impairs the nuclear localization of the ependymoma-associated ZFTA-RELA fusion protein and NF-κB activation

This study identifies microtubule and dynein inhibitors that block ZFTA-RELA nuclear localization and NF-κB activation in ependymoma cells.

Aurora kinase A is a synthetic lethal target in FANCA-deficient cancers

The paper identifies Aurora kinase A as a synthetic lethal target in FANCA-deficient cancers and shows AURKA inhibition induces chromosomal instability and G2/M arrest in these cells.

Coupling of tubulin acetylation to microtubule stabilization through molecular mimicry

This study identifies MTCL1 as a factor linking microtubule stabilization and acetylation, showing its role in neuronal function through molecular mimicry.

Emergence of large-scale polar microtubule swarms for dense molecular transport

This study demonstrates the formation of large-scale polar microtubule swarms that enable dense molecular transport through motor-driven activity and filament regeneration.

The N-terminus of SAS-1 promotes microtubule-dependent centriolar satellite formation

This study identifies SAS-1 and SSNA-1 as core components of centriolar satellite-like structures in C. elegans and shows the N-terminus of SAS-1 is essential for satellite formation and microtubule binding.

Lateral interactions override nucleotide state in determining FtsZ filament curvature

This study shows that lateral interactions between FtsZ protofilaments override nucleotide state in determining filament curvature, with implications for Z-ring assembly and constriction.

NuSAP1 promotes bipolar spindle assembly in Trypanosoma brucei by bundling spindle microtubules

This study identifies NuSAP1's role in promoting bipolar spindle assembly in Trypanosoma brucei by bundling microtubules and facilitating spindle pole complex formation.

Functions of TIAM1 at the interface of centriole assembly and autolysosome cycling

The paper investigates TIAM1's role in linking centriole assembly with autolysosome cycling, finding that TIAM1 depletion disrupts PLK4 distribution and alters autophagy-lysosome homeostasis in mouse cells.

Replication stress links Geminin depletion to centrosome amplification

This study shows that Geminin depletion causes replication stress, leading to centrosome amplification and impaired primary cilium assembly in mouse cells.

Age-associated changes to mouse oocyte meiotic spindle properties revealed through in situ measurements

This study quantifies age-related changes in the mechanical properties of mouse oocyte spindles using a combination of coarse-grained modeling and in situ experiments.

Remote homology and functional genetics unmask deeply preserved Scm3/HJURP orthologs in metazoans

The paper identifies previously unknown Scm3/HJURP orthologs in metazoans using remote homology, structural modeling, and functional genetics, revealing their conserved role in centromere function despite sequence divergence.

A Cell Size-Dependent Competition Between Geometry and Polarity Governs Nuclear and Spindle positioning in Early Embryos

The paper investigates how nuclear and spindle positioning in early sea urchin embryos shifts from geometry-based to polarity-based control as cells shrink during development.

RhoGAP54D promotes cell size asymmetry and inhibits pulsatile Myosin activity in Drosophila neural stem cells

This study identifies RhoGAP54D as a regulator of Myosin dynamics in Drosophila neuroblasts, showing it suppresses Myosin activity during interphase and metaphase and initiates directional Myosin flow during anaphase to promote cell size asymmetry.

Adaptable centriole biogenesis via the intrinsically disordered protein ALMS1

This study identifies ALMS1 as an intrinsically disordered protein that mediates cartwheel dynamics during centriole biogenesis, showing its role in forming and disassembling cartwheel seeds and enabling adaptable centriole formation.