Microtubule and mitosis dynamics

CLASP2 promotes repair of kinesin-1 damage to the microtubule lattice

This study identifies CLASP2 as a factor that facilitates microtubule repair in response to damage caused by kinesin-1 motor proteins.

The kinetochore proteins Ndc80 and Dsn1 are required for survival of postmitotic neurons

This study shows that Dsn1 and Ndc80 are essential for postmitotic neuron survival, distinct from their role in synaptogenesis, and identifies domain-specific functions in neuronal viability.

MAP1A cleavage regulates TRIM46 transport to the axon in mature neurons

The paper identifies MAP1A cleavage as essential for TRIM46 transport to the axon in mature neurons, supporting axon morphology through a newly defined transport complex.

The TIRR-53BP1 axis controls PLK1 spatiotemporal regulation during mitosis

This study shows that the TIRR-53BP1 axis regulates PLK1 activity during mitosis, affecting chromosome segregation and kinetochore assembly.

Chromosome condensation mechanically primes the nucleus for mitosis

The paper shows chromosome condensation increases nuclear envelope tension, which is necessary for cyclin B1 translocation and dynein loading, enabling timely mitotic entry.

Microtubule stiffening by doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

This study shows that ZYG-8 increases microtubule rigidity, which is essential for proper spindle orientation during C. elegans zygote division, and links microtubule mechanics to spindle positioning.

The kinesin-4 family member KIF27 regulates mitotic progression, cytokinesis and genome stability

This study identifies KIF27 as a regulator of mitotic progression, cytokinesis, and genome stability through its dynamic localization and functional roles in cell division.

Low-affinity binding motif in microtubule plus-end condensates specializes microtubule function

The paper identifies a low-affinity KDK motif in Kar9 that specializes +TIP body function by modulating microtubule dynamics through multivalent interactions with Bik1.

Rho1 and Rgf3 regulate the expansion of the nuclear envelope during fission yeast mitosis/cytokinesis

This study identifies Rho1 and Rgf3's role in nuclear envelope expansion during fission yeast mitosis by modulating phospholipid synthesis via the Kennedy pathway.

SETDB1 promotes tubulin deacetylation and Golgi fragmentation by HDAC6

This paper identifies SETDB1 as a regulator of microtubule dynamics and Golgi organization through its interaction with HDAC6, promoting tubulin deacetylation and Golgi fragmentation.

Structural basis of microtubule destabilization by GTP hydrolysis

This study uses cryo-EM to resolve microtubule structures in different nucleotide states, revealing how GTP hydrolysis affects lattice stability and microtubule dynamics.

Mechanical tension expands the microtubule lattice stepwise and modulates kinesin-1 binding in an isoform-dependent manner

This paper measures how mechanical tension affects microtubule lattice expansion and kinesin-1 binding, showing isoform-dependent responses and a critical switching force.

Mechanical contributions of the Myp2 tail revealed by coiled-coil force sensors

This study measures forces on Myp2 in fission yeast using force sensors, showing the C-terminal tail significantly contributes to force transmission during cytokinesis.

Capillary bundling of microtubules by condensates

This study demonstrates that microtubule bundling can occur through condensate-mediated capillary forces, independent of bulk protein concentration.

A Modular Chromosomal Passenger Complex Rewires Chromosome Segregation in Plasmodium berghei

This study identifies ARK1 as a key regulator of chromosome segregation in Plasmodium berghei, showing stage-specific rewiring of the Chromosomal Passenger Complex during mitosis and meiosis.

A combination of EWSR1-FLI1 with loss of one EWSR1 allele promotes damage in the mitotic spindle and sensitization to microtubule-destabilizing agent

The paper shows that combining EWSR1-FLI1 expression with EWSR1 knockdown increases mitotic spindle damage and sensitivity to microtubule-destabilizing agents in Ewing sarcoma cells.

Microtubule Acetylation Regulates the Malignant Phenotype of Glioblastoma and is a Promising Therapeutic Target

This study identifies ATAT1 as a therapeutic target in glioblastoma by showing its role in promoting tumor invasion, proliferation, and resistance, with potential for combination therapies.

Architecture and Function of Holocentric CENP-A-Independent Kinetochores

The paper determines the cryo-EM structure of the holocentric CCAN from Bombyx mori, revealing a self-contained dimer that forms a point-centromere-like architecture and incorporates novel Centromeric Subunit proteins.

Mutating different α-tubulin acetylation sites has distinct effects on axon terminal morphogenesis in Drosophila melanogaster

SPACA9 Acts as a Molecular Staple Modulating Microtubule Dynamic Instability

This study identifies SPACA9 as a molecular staple that stabilizes microtubule ends and inhibits dynamic instability without affecting mechanical resilience.

Non-cell-autonomous mechanisms of tumor initiation and relapse by chromosomal instability

This study shows that chromosomal instability (CIN) can both promote and suppress tumor formation depending on its level, with high CIN acting non-cell-autonomously to drive tumor relapse.

Novel KIF22 Variants Disrupt Mitosis in Human Chondrocytes and Expand SEMDJL2 Mechanisms

This study examines how different KIF22 variants affect mitosis in human chondrocytes, identifying two mechanistic classes of dysregulation associated with SEMDJL2.

The consequences of array size for human centromere performance in mitosis

This study investigates how variations in centromeric alpha-satellite array size affect centromere function during mitosis, finding that smaller arrays are more prone to missegregation and cohesion fatigue.

An atlas of microtubule lattice parameters regulated through ligand binding to the microtubule stabilizing sites.

This paper investigates how microtubule-stabilizing ligands alter lattice conformation and function, showing they stabilize distinct longitudinal states that affect nucleotide hydrolysis and motor protein interactions.

Multipolar spindle assembly and mitotic slippage underlie symbiont-mediated asexual reproduction

The paper investigates how Wolbachia induces asexual reproduction in parasitoid wasps by causing mitotic failure and slippage through altered microtubule organizing center biology.

The centriculum, a membrane reticulum that surrounds C. elegans centrosomes, may serve as a microtubule filter

This study identifies the centriculum as a microtubule-filtering membrane reticulum around C. elegans centrosomes, showing its size depends on microtubules and its porous structure allows selective microtubule passage.

Contractile perinuclear actomyosin network promotes peripheral and polar chromosome interaction with the mitotic spindle

The paper shows that PANEM contraction repositions peripheral and polar chromosomes to enhance their interaction with the mitotic spindle, ensuring correct segregation and reducing chromosomal instability in cancer cells.

A dynein-driven nucleokinesis program enables neural crest migration through confined tissues in vivo

This study identifies a dynein-driven microtubule-based mechanism for nuclear translocation in zebrafish neural crest cells during confined migration.

Tagging C. elegans septins disrupts cytoskeletal scaffolding but not post-embryonic roles

This study shows fluorescent tagging of C. elegans septins disrupts cytokinesis but not post-embryonic development, indicating distinct functional roles in different developmental stages.

Reconstruction of septin higher-order nano-size structures in ovarian cancer cells uncover susceptibility to the septin-targeting small molecule UR214-9

This paper demonstrates that UR214-9 disrupts septin hetero-octamerization, leading to aberrant structures and impairing cancer cell functions.