Microtubule and mitosis dynamics

Spatiotemporal regulation of LGN/NuMA and astral microtubules generate mirror symmetric spindle rotations

The paper investigates spindle positioning in ascidian embryos, showing that cortical pulling and cytoplasmic dynein forces act sequentially to generate mirror symmetric and unequal divisions.

KIFC1 overexpression induces monopolar spindles by preventing centrosome separation during rapid cleavage divisions

This study shows that KIFC1 overexpression in medaka embryos causes monopolar spindles by preventing centrosome separation, leading to chromosome mis-segregation and embryonic lethality.

Spindle scaling promotes adaptation to polyploidy

This study shows that spindle scaling enables adaptation to polyploidy by modulating microtubule dynamics and architecture across different ploidy levels.

Dissection of centrosomal γ-TuRC activation pathways controlling microtubule density in interphase cells

The paper investigates pathways controlling microtubule density in interphase cells by disrupting CAMSAP and γ-TuRC pathways, revealing redundant activation mechanisms.

Kinetochore diversification in Naegleria reveals plasticity in the kinetochore-microtubule interface

This study investigates kinetochore-microtubule interface plasticity in Naegleria by analyzing its kinetochore structure and function, revealing charge-based binding mechanisms that allow sequence divergence.

The centromere localization domain of kinetoplastid kinetochore protein KKT2 recognizes the free N-terminus of histone H3

This study shows that the KKT2 CL domain binds histone H3 N-terminus and that methylation of this region disrupts binding, suggesting a mechanism for kinetochore assembly in kinetoplastids.

Lamin B1-dependent regulation of human separase in mitosis

This study identifies lamin B1 as a regulator of separase activity during mitosis, showing that lamin B1 depletion or overexpression disrupts chromosome separation and centrosome stability.

Local fluidization of an active cytoplasmic gel partitions large cells

The paper shows that cytoplasm in early embryos behaves as a locally switchable active material, enabling long-range transport of components during cleavage.

A Phosphorylation Switch Governs KIF11's Mechanical Output During Mitosis

This study investigates how phosphorylation at Y211 regulates KIF11's mechanical properties during mitosis, showing it modulates motor velocity, force generation, and spindle dynamics.

Microtubule occupancy at kinetochores links checkpoint silencing with mitotic memory

The paper links SAC silencing with mitotic memory through microtubule occupancy at kinetochores, showing that high occupancy enables timely silencing and prevents mitotic stopwatch-induced proliferation block.

NET1 mRNA localization to the midbody is required for ARP2/3-dependent initiation of mitotic abscission

This study identifies NET1 mRNA localization to the midbody as essential for ARP2/3-dependent mitotic abscission by regulating protein expression and actin dynamics.

Mitotic adaptations shape acquired resistance and vulnerabilities to KIF18A inhibition in cancer

This study identifies resistance mechanisms and vulnerabilities to KIF18A inhibition in cancer cells using CRISPR screening and drug adaptation.

Synthetic cargo adaptors reveal molecular features that can enhance dynein activation

This study engineers synthetic cargo adaptors to investigate how they modulate dynein activation and transport properties in vitro and in cells.

Chemoproteomics identifies a pyrimidopyrimidine analogue as a tubulin-tyrosine ligase binder

The paper identifies CEM198 as a TTL binder through chemoproteomics, showing it disrupts microtubule dynamics via dual mechanisms in different cell lines.

Native yeast kinetochore structures identify an essential inner kinetochore interaction

The paper identifies an essential Ndc10 trimerization interaction in yeast kinetochores that stabilizes centromeric DNA and facilitates kinetochore assembly.

Curved microtubule regions mark sites of lattice compaction in cells and neurons

The paper identifies curved microtubule regions as markers of lattice compaction and shows how lattice states regulate protein recruitment and signaling in cells and neurons.

An interdependent Cbf1-CCAN interaction stabilizes the budding yeast kinetochore

This study identifies an interdependent Cbf1-CCAN interaction that stabilizes the budding yeast kinetochore by coupling transcriptional roadblocking to inner kinetochore assembly.

Microtubule Lattice Spacing Governs MAP-Motor Regulation

The paper investigates how microtubule lattice spacing affects MAP binding and kinesin-1 motility, revealing that lattice spacing, not tubulin PTMs, primarily regulates these processes.

An architectural switch in the evolution of the γ-tubulin ring complex

This study presents the cryo-EM structure of the C. elegans γ-TuRC, revealing how it templates 11-protofilament microtubules through a divergent 4-spoked assembly.

An in vivo examination of Dynein-Cargo complex formation.

This study identifies Bicaudal-D and Centrocortin as dynein adaptors in Drosophila egg chambers, showing distinct mechanisms for their interaction with dynein and implications for cargo transport.

Elevated Levels of Mcm7 Disrupt Microtubule and Nucleolar Homeostasis to Drive Genome Instability and Cell Death

This study shows that elevated Mcm7 levels disrupt microtubule and nucleolar homeostasis, leading to genome instability and cell death in yeast, with implications for human cancer.

ZNF512B associates with mitotic spindles, regulates metaphase exit and is crucial for stem cell differentiation

This study identifies ZNF512B as a spindle-associated protein that regulates metaphase exit and stem cell differentiation, independent of its zinc finger domains and NuRD complex interaction.

Smc5/6 - microtubules binding shapes pericentromeric chromatin

This study shows that Smc5/6 microtubule binding constrains chromatin dynamics and promotes pericentromeric folding in yeast, safeguarding genome stability during mitosis.

Identification and functional analysis of αKNL2 genes in cowpea

This study identifies two KNL2 genes in cowpea, characterizes their expression and subcellular localization, and examines their functional roles in centromere assembly and seed development.

Dissecting structural and functional determinants of microtubule stabilization through guided chemical modulation

The paper designs and characterizes PTX analogues to separate MT stabilization from structural changes, showing that compound 1b preserves native MT structure and physiological Tau dynamics.

The extraordinary robustness of mitotic spindle assembly to microtubule dynamics plasticity revealed by end-binding proteins and tubulin tagging

The paper compares microtubule dynamics in C. elegans embryos using fluorescent labels and finds high variability, with spindle assembly robustness despite dynamic changes and potential issues with tagged end-binding proteins.

Microtubule Engagement by the WD40-Containing Tail of Kinesin-4 KIF21B

This study uses cryo-EM and cryo-ET to reveal how the WD40 domain of KIF21B interacts with microtubules, identifying two binding states and suggesting its role in microtubule regulation.

Calmodulin requires Ca2+ to integrate into the spindle pole body and modulate the cytokinetic ring constriction in fission yeast cells

Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function

This study shows mammalian metaphase kinetochores are elastic and require condensin for structural stability and microtubule attachment under force.

Klp61f and ncd function as an accelerator and brake to regulate myonuclear spacing

This study identifies Klp61f and ncd as kinesins that regulate myonuclear spacing through opposing forces during development and in differentiated myofibers.